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AB171954

Anti-ZP1 antibody [EPR10827]

0

(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal ZP1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Zona pellucida sperm-binding protein 1, Zona pellucida glycoprotein 1, Zp-1, ZP1

1 Images
Western blot - Anti-ZP1 antibody [EPR10827] (AB171954)
  • WB

Supplier Data

Western blot - Anti-ZP1 antibody [EPR10827] (AB171954)

All lanes:

Western blot - Anti-ZP1 antibody [EPR10827] (ab171954) at 1/1000 dilution

Lane 1:

NIH: OVCAR-3 cell lysate at 10 µg

Lane 2:

SK-OV-3 cell lysate at 10 µg

Lane 3:

LOVO cell lysate at 10 µg

Lane 4:

Human ovary cancer tissue lysate at 10 µg

Predicted band size: 70 kDa

false

  • Carrier free

    Anti-ZP1 antibody [EPR10827] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10827

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZP1 also known as Zona Pellucida Glycoprotein 1 is a protein that plays a mechanical role in the structure of the zona pellucida which is the extracellular matrix surrounding mammalian oocytes. This protein has a molecular mass of approximately 66 kDa. ZP1 is expressed in the ovary particularly by ovarian oocytes. It is one of the critical components that provides structural integrity to the zona pellucida assisting in species-specific sperm binding and initial protection of the oocyte.
Biological function summary

The protein ZP1 facilitates oocyte-sperm interactions during fertilization. It works as part of a complex with other zona pellucida glycoproteins like ZP2 and ZP3. This complex is essential for the cohesion of the zona pellucida enabling its protective and interactive roles. ZP1 helps maintain the mechanical properties of the zona pellucida essential for successful fertilization and eventual embryo development.

Pathways

The protein ZP1 is involved in reproductive pathways particularly those regulating fertilization and early embryonic development. ZP1 interacts closely with ZP2 and ZP3 to form the zona pellucida matrix. This matrix is critical for the sperm binding and zona reaction pathways ensuring only one sperm fertilizes the oocyte. Without the proper function of ZP1 these pathways can be disrupted leading to fertility issues.

Abnormalities in ZP1 can lead to fertilization problems such as infertility or early embryonic loss. Mutations or deficiencies in ZP1 have been associated with conditions like oocyte maturation defects. These defects may connect with alterations in other zona pellucida proteins including ZP2 and ZP3 which can likewise affect fertility. Addressing these disorders often involves understanding the specific roles and interactions of ZP1 with these proteins aiming for potential therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP1 ensures the structural integrity of the zona pellucida.
See full target information ZP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 35:1219-29 PubMed25760093

2015

Stem cells derived from human first-trimester umbilical cord have the potential to differentiate into oocyte-like cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Hu,Hua Lu,Sheng Cao,Yan-Li Deng,Qi-Jia Li,Qian Wan,Shang-Mian Yie
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com