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AB97691

Anti-ZPBP1 antibody

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(2 Publications)

Rabbit Polyclonal ZPBP1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZPBP aa 1-300.

View Alternative Names

ZPBP1, ZPBP, Zona pellucida-binding protein 1, Inner acrosomal membrane IAM38, Sp38

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPBP1 antibody (AB97691)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPBP1 antibody (AB97691)

ab97691 at 1/100 dilution staining ZPBP1 in paraffin-embedded SCC25 xenograft tissue by immunohistochemistry.

Western blot - Anti-ZPBP1 antibody (AB97691)
  • WB

Unknown

Western blot - Anti-ZPBP1 antibody (AB97691)

12% SDS-PAGE.

All lanes:

Western blot - Anti-ZPBP1 antibody (ab97691) at 1/500 dilution

Lane 1:

A431 whole cell lysate at 30 µg

Lane 2:

HeLa S3 whole cell lysate at 30 µg

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ZPBP aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9BS86

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZPBP1 also known as Zona Pellucida Binding Protein 1 functions mechanically by interacting with the zona pellucida an important glycoprotein layer surrounding the oocyte. This protein has a molecular mass of approximately 67 kDa. It is expressed in the testis especially in developing sperm cells. ZPBP1 facilitates sperm binding to the oocyte's protective layer an important process for successful fertilization. Through this interaction it influences the acrosome reaction essential for penetrative capabilities of sperm cells.
Biological function summary

ZPBP1 plays a significant role in sperm-egg recognition and fusion. This protein is part of a complex involving other zona pellucida binding proteins including acrosomal proteins. It contributes to the structural integrity and function of the acrosome cap in spermatozoa. Proper functioning of ZPBP1 ensures sperm can traverse their way to the oocyte which is critical for normal reproductive processes. In mammals this binding action initiates biochemical events within sperm that are indispensable for crossing the zona pellucida.

Pathways

ZPBP1 participates in the reproductive signaling pathway. This protein engages with pathways that regulate egg-sperm interactions and fertilization. It connects with other proteins such as IZUMO1 and ADAM3 which are essential in the sperm-egg adhesion process. By acting within these pathways ZPBP1 ensures effective communication and molecular interactions between sperm and egg during fertilization. It also plays a part in regulating the acrosome reaction which is necessary for the sperm to penetrate the oocyte.

ZPBP1 mutations or dysregulations contribute to male infertility. Such conditions often involve impaired sperm binding compromising the fertilization process. Defects in this protein also associate with certain cases of azoospermia a condition characterized by absence of sperm in the ejaculate. The protein ZPBP1 is connected to other sperm-related proteins such as SPAM1 due to their role in sperm motility and fusion and defects may lead to reproductive challenges. Understanding ZPBP1 can help in developing potential diagnostic or therapeutic approaches for related reproductive disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in acrosome compaction and sperm morphogenesis (PubMed : 21911476). Is implicated in sperm-oocyte interaction during fertilization (By similarity).
See full target information ZPBP
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35563519

2022

New Insight on the In Vitro Effects of Melatonin in Preserving Human Sperm Quality.

Applications

Unspecified application

Species

Unspecified reactive species

Sergio Minucci,Massimo Venditti

Clinical proteomics 15:27 PubMed30166971

2018

iTRAQ-based analysis of sperm proteome from normozoospermic men achieving the rescue-ICSI pregnancy after the IVF failure.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Liu,Gensheng Liu,Juan Liu,Peng Zhu,Jiahui Wang,Yanwei Wang,Wenting Wang,Ning Li,Xuebo Wang,Chenglin Zhang,Xiaofang Shen,Fujun Liu
View all publications
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Product promise

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