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AB181132

Anti-ZPI antibody [EPR12072]

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(2 Publications)

Rabbit Recombinant Monoclonal ZPI antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

ZPI, UNQ707/PRO1358, SERPINA10, Protein Z-dependent protease inhibitor, PZ-dependent protease inhibitor, PZI, Serpin A10

2 Images
Immunoprecipitation - Anti-ZPI antibody [EPR12072] (AB181132)
  • IP

Supplier Data

Immunoprecipitation - Anti-ZPI antibody [EPR12072] (AB181132)

Western blot analysis of Human plasma lysate immunoprecipitated with ab181132 at 1/60 dilution (Lane 1). Lane 2 : Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG was used at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-ZPI antibody [EPR12072] (ab181132)

Predicted band size: 50 kDa

false

Western blot - Anti-ZPI antibody [EPR12072] (AB181132)
  • WB

Supplier Data

Western blot - Anti-ZPI antibody [EPR12072] (AB181132)

All lanes:

Western blot - Anti-ZPI antibody [EPR12072] (ab181132) at 1/1000 dilution

Lane 1:

Human plasma lysate at 10 µg

Lane 2:

Human serum lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12072

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZPI also known as Protein Z-dependent protease inhibitor is a serine protease inhibitor with a mass of approximately 72 kDa. It inhibits factor Xa in a process that requires Protein Z and calcium ions. ZPI is mainly expressed in the liver and its presence has been noted in plasma. Structurally ZPI acts by forming a complex with factor Xa in the presence of protein Z leading to the inactivation of the coagulation pathway's enzymatic activity effectively reducing clot formation under physiological conditions.
Biological function summary

ZPI plays a significant role in the regulation of hemostasis controlling blood clot formation by inhibiting excess factor Xa activity. This inhibition prevents unnecessary clotting maintaining fluidity and preventing vascular occlusion. ZPI is particularly important in the context of the coagulation cascade where it forms part of a complex with Protein Z and calcium ions offering a fine balance to the procoagulant processes mediated by thrombin and factor Xa.

Pathways

The actions of ZPI largely influence the intrinsic and extrinsic coagulation pathways. Its primary function is to regulate the activity of factor Xa significantly impacting the common pathway of coagulation. Through these pathways ZPI is closely related to proteins like factor VIIa and factor IXa participating in a delicate interplay necessary for maintaining hemostatic balance and preventing thrombosis or hemorrhage.

ZPI's functional integrity is linked to conditions such as thrombophilia where an inability to effectively inhibit factor Xa can lead to increased thrombotic events. Furthermore deficiencies or malfunctions of ZPI have been associated with a heightened risk of venous thromboembolism (VTE). Altered interactions between ZPI and protein Z or factor Xa can contribute to these conditions highlighting the importance of balanced protease inhibitor activity in maintaining vascular health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibits activity of the coagulation protease factor Xa in the presence of PROZ, calcium and phospholipids. Also inhibits factor XIa in the absence of cofactors.
See full target information SERPINA10
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:27448 PubMed39523403

2024

Assessing expression patterns of PTGR1, a potential biomarker for acylfulven sensitivity in urothelial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dag Rune Stormoen,Signe Lehn,Kent W Mouw,Zoltan Szallasi,Linea Cecilie Melchior,Line Hammer Dohn,Judit Börcsok,Maria Rossing,Birgitte Grønkaer Toft,Helle Pappot

Ginekologia polska 93:987-992 PubMed35072242

2022

Expression and diagnostic value of plasma protein Z and protein Z-dependent protease inhibitor in fetal growth restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Ci Pan,Yulian Huang,Xiaopei Guo,Cunhua Gu,Jing Guo,Xiaohua Luo
View all publications
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Product promise

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