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AB250370

Anti-ZPI antibody [EPR12072] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ZPI antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ZPI, UNQ707/PRO1358, SERPINA10, Protein Z-dependent protease inhibitor, PZ-dependent protease inhibitor, PZI, Serpin A10

2 Images
Immunoprecipitation - Anti-ZPI antibody [EPR12072] - BSA and Azide free (AB250370)
  • IP

Supplier Data

Immunoprecipitation - Anti-ZPI antibody [EPR12072] - BSA and Azide free (AB250370)

This data was developed using ab181132, the same antibody clone in a different buffer formulation.

Western blot analysis of Human plasma lysate immunoprecipitated with ab181132 at 1/60 dilution (Lane 1). Lane 2 : Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG was used at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-ZPI antibody [EPR12072] (<a href='/en-us/products/primary-antibodies/zpi-antibody-epr12072-ab181132'>ab181132</a>)

Predicted band size: 50 kDa

false

Western blot - Anti-ZPI antibody [EPR12072] - BSA and Azide free (AB250370)
  • WB

Supplier Data

Western blot - Anti-ZPI antibody [EPR12072] - BSA and Azide free (AB250370)

This data was developed using ab181132, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ZPI antibody [EPR12072] (<a href='/en-us/products/primary-antibodies/zpi-antibody-epr12072-ab181132'>ab181132</a>) at 1/1000 dilution

Lane 1:

Human plasma lysate at 10 µg

Lane 2:

Human serum lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12072

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250370 is the carrier-free version of ab181132.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZPI also known as Protein Z-dependent protease inhibitor is a serine protease inhibitor with a mass of approximately 72 kDa. It inhibits factor Xa in a process that requires Protein Z and calcium ions. ZPI is mainly expressed in the liver and its presence has been noted in plasma. Structurally ZPI acts by forming a complex with factor Xa in the presence of protein Z leading to the inactivation of the coagulation pathway's enzymatic activity effectively reducing clot formation under physiological conditions.
Biological function summary

ZPI plays a significant role in the regulation of hemostasis controlling blood clot formation by inhibiting excess factor Xa activity. This inhibition prevents unnecessary clotting maintaining fluidity and preventing vascular occlusion. ZPI is particularly important in the context of the coagulation cascade where it forms part of a complex with Protein Z and calcium ions offering a fine balance to the procoagulant processes mediated by thrombin and factor Xa.

Pathways

The actions of ZPI largely influence the intrinsic and extrinsic coagulation pathways. Its primary function is to regulate the activity of factor Xa significantly impacting the common pathway of coagulation. Through these pathways ZPI is closely related to proteins like factor VIIa and factor IXa participating in a delicate interplay necessary for maintaining hemostatic balance and preventing thrombosis or hemorrhage.

ZPI's functional integrity is linked to conditions such as thrombophilia where an inability to effectively inhibit factor Xa can lead to increased thrombotic events. Furthermore deficiencies or malfunctions of ZPI have been associated with a heightened risk of venous thromboembolism (VTE). Altered interactions between ZPI and protein Z or factor Xa can contribute to these conditions highlighting the importance of balanced protease inhibitor activity in maintaining vascular health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibits activity of the coagulation protease factor Xa in the presence of PROZ, calcium and phospholipids. Also inhibits factor XIa in the absence of cofactors.
See full target information SERPINA10
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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