AB273627

Anti-ZPI antibody [EPR23683-118]

20ul selling size
BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal ZPI antibody. Suitable for IP, WB, IHC-P and reacts with Human, Rat, Mouse samples.

View Alternative Names

ZPI, UNQ707/PRO1358, SERPINA10, Protein Z-dependent protease inhibitor, PZ-dependent protease inhibitor, PZI, Serpin A10

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (AB273627)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (AB273627)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling ZPI with ab273627 at 1/1000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human liver (PMID : 20024489). The section was incubated with ab273627 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (AB273627)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling ZPI with ab273627 at 1/1000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on pancreas islets (PMID : 16728581). The section was incubated with ab273627 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (AB273627)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling ZPI with ab273627 at 1/1000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab273627 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Negative control : No staining on human cererbum (PMID : 20024489).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (AB273627)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling ZPI with ab273627 at 1/1000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on plasma in human placenta. The section was incubated with ab273627 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Unknown

Immunoprecipitation - Anti-ZPI antibody [EPR23683-118] (AB273627)

ZPI was immunoprecipitated from 0.35 mg human plasma with ab273627 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273627 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Human plasma lysate 10μg

Lane 2 : ab273627 IP in human plasma

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab273627 in human plasma

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

The expression profile observed is consistent with what has been described in the literature (PMID : 10460162).

All lanes:

Immunoprecipitation - Anti-ZPI antibody [EPR23683-118] (ab273627)

Predicted band size: 50 kDa

Observed band size: 75 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (AB273627)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling ZPI with ab273627 at 1/1000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat liver (PMID : 20024489). The section was incubated with ab273627 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Lab

Western blot - Anti-ZPI antibody [EPR23683-118] (AB273627)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 10460162, 9689066).

Negative control : Human brain (PMID : 10460162).

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 37 seconds.

All lanes:

Western blot - Anti-ZPI antibody [EPR23683-118] (ab273627) at 1/1000 dilution

Lane 1:

Human liver cancer tissue lysate at 20 µg

Lane 2:

Human brain tissue lysate at 20 µg

Secondary

All lanes:

VeriBlot for IP secondary antibody(HRP)(<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 75 kDa

false

Lab

Western blot - Anti-ZPI antibody [EPR23683-118] (AB273627)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. The expression profile observed is consistent with what has been described in the literature (PMID : 10460162, 9689066). Exposure time : 3 minutes.

All lanes:

Western blot - Anti-ZPI antibody [EPR23683-118] (ab273627) at 1/1000 dilution

Lane 1:

Human serum at 20 µL

Lane 2:

Human plasma at 20 µL

Secondary

All lanes:

VeriBlot for IP secondary antibody(HRP)(<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 75 kDa

false

Lab

Western blot - Anti-ZPI antibody [EPR23683-118] (AB273627)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 10460162, 9689066).

Exposure times : Lane 1 : 26 seconds; Lane 2-3 : 5.5 seconds.

All lanes:

Western blot - Anti-ZPI antibody [EPR23683-118] (ab273627) at 1/1000 dilution

Lane 1:

Mouse plasma at 20 µL

Lane 2:

Rat serum at 20 µL

Lane 3:

Rat plasma at 20 µL

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 50 kDa

Observed band size: 75 kDa

false

Carrier free

Anti-ZPI antibody [EPR23683-118] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23683-118

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZPI also known as Protein Z-dependent protease inhibitor is a serine protease inhibitor with a mass of approximately 72 kDa. It inhibits factor Xa in a process that requires Protein Z and calcium ions. ZPI is mainly expressed in the liver and its presence has been noted in plasma. Structurally ZPI acts by forming a complex with factor Xa in the presence of protein Z leading to the inactivation of the coagulation pathway's enzymatic activity effectively reducing clot formation under physiological conditions.

Biological function summary

ZPI plays a significant role in the regulation of hemostasis controlling blood clot formation by inhibiting excess factor Xa activity. This inhibition prevents unnecessary clotting maintaining fluidity and preventing vascular occlusion. ZPI is particularly important in the context of the coagulation cascade where it forms part of a complex with Protein Z and calcium ions offering a fine balance to the procoagulant processes mediated by thrombin and factor Xa.

Pathways

The actions of ZPI largely influence the intrinsic and extrinsic coagulation pathways. Its primary function is to regulate the activity of factor Xa significantly impacting the common pathway of coagulation. Through these pathways ZPI is closely related to proteins like factor VIIa and factor IXa participating in a delicate interplay necessary for maintaining hemostatic balance and preventing thrombosis or hemorrhage.

ZPI's functional integrity is linked to conditions such as thrombophilia where an inability to effectively inhibit factor Xa can lead to increased thrombotic events. Furthermore deficiencies or malfunctions of ZPI have been associated with a heightened risk of venous thromboembolism (VTE). Altered interactions between ZPI and protein Z or factor Xa can contribute to these conditions highlighting the importance of balanced protease inhibitor activity in maintaining vascular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibits activity of the coagulation protease factor Xa in the presence of PROZ, calcium and phospholipids. Also inhibits factor XIa in the absence of cofactors.

See full target information SERPINA10

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZPI antibody [EPR23683-118]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (AB273627)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (AB273627)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (AB273627)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (AB273627)

Immunoprecipitation - Anti-ZPI antibody [EPR23683-118] (AB273627)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPI antibody [EPR23683-118] (AB273627)

Western blot - Anti-ZPI antibody [EPR23683-118] (AB273627)

Western blot - Anti-ZPI antibody [EPR23683-118] (AB273627)

Western blot - Anti-ZPI antibody [EPR23683-118] (AB273627)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise