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AB248762

Anti-ZPR1 antibody [EPR7595] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ZPR1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ZNF259, ZPR1, Zinc finger protein ZPR1, Zinc finger protein 259

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPR1 antibody [EPR7595] - BSA and Azide free (AB248762)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPR1 antibody [EPR7595] - BSA and Azide free (AB248762)

This data was developed using ab134970, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling ZPR1 with ab134970 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-ZPR1 antibody [EPR7595] - BSA and Azide free (AB248762)
  • WB

Unknown

Western blot - Anti-ZPR1 antibody [EPR7595] - BSA and Azide free (AB248762)

This data was developed using ab134970, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ZPR1 antibody [EPR7595] (<a href='/en-us/products/primary-antibodies/zpr1-antibody-epr7595-ab134970'>ab134970</a>) at 1/1000 dilution

Lane 1:

Human stomach lysate at 10 µg

Lane 2:

Human fetal liver lysate at 10 µg

Lane 3:

Human colon cancer lysate at 10 µg

Lane 4:

BxPC3 lysate at 10 µg

Lane 5:

HepG2 lysate at 10 µg

Lane 6:

Caco2 lysate at 10 µg

Lane 7:

MCF7 lysate at 10 µg

Lane 8:

Jurkat lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 51 kDa

Observed band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7595

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab248762 is the carrier-free version of ab134970.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZPR1 also known as Zinc finger protein 1 is a protein weighing approximately 54 kDa. This protein mainly exists in the cytoplasm and nucleus of cells. ZPR1 interacts with the survival motor neuron (SMN) protein and cellular growth factors playing key roles in signal transduction and cellular proliferation processes. Its expression is highly regulated in a variety of tissues including brain liver and muscle.
Biological function summary

The actions of ZPR1 extend beyond simple binding interactions. ZPR1 assists in the formation and stability of SMN protein complexes critical for the assembly of small nuclear ribonucleoproteins (snRNPs). The protein is essential for proper localization of SMN in the nucleus a function that supports RNA splicing machinery maintenance. ZPR1 acts together with other constituents ensuring cellular communication and growth.

Pathways

ZPR1 operates mainly in pathways related to signal transduction and RNA processing. ZPR1 interacts in the PI3K/AKT pathway which is significant for cell growth and survival. Additionally the protein is linked to the TGF-beta signaling pathway impacting cell differentiation and tissue homeostasis. These interactions involve related proteins such as GRB2 and SMAD3 which further integrate ZPR1's role in these pathways.

The impairment of ZPR1 function shows relevance to neurodegenerative diseases specifically spinal muscular atrophy (SMA). This connection arises from its interaction with the SMN protein which is deficient in SMA patients. Additionally alterations in ZPR1 activity may contribute to certain cancer types due to its influence on cell proliferation pathways. Its pathway associations make ZPR1 a subject of interest in disease research linking to proteins involved in oncogenic processes like PI3K.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a signaling molecule that communicates proliferative growth signals from the cytoplasm to the nucleus. It is involved in the positive regulation of cell cycle progression (PubMed : 29851065). Plays a role for the localization and accumulation of the survival motor neuron protein SMN1 in sub-nuclear bodies, including gems and Cajal bodies. Induces neuron differentiation and stimulates axonal growth and formation of growth cone in spinal cord motor neurons. Plays a role in the splicing of cellular pre-mRNAs. May be involved in H(2)O(2)-induced neuronal cell death.
See full target information ZPR1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 7:95 PubMed37723227

2023

Targeted single-cell proteomic analysis identifies new liquid biopsy biomarkers associated with multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia M Setayesh,Libere J Ndacayisaba,Kate E Rappard,Valerie Hennes,Luz Yurany Moreno Rueda,Guilin Tang,Pei Lin,Robert Z Orlowski,David E Symer,Elisabet E Manasanch,Stephanie N Shishido,Peter Kuhn
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com