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AB174657

Anti-ZPR9 antibody

5

(1 Review)

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(1 Publication)

Rabbit Polyclonal ZPR9 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ZNF622 aa 100-200.

View Alternative Names

ZPR9, ZNF622, Cytoplasmic 60S subunit biogenesis factor ZNF622, Zinc finger protein 622, Zinc finger-like protein 9

2 Images
Immunoprecipitation - Anti-ZPR9 antibody (AB174657)
  • IP

Supplier Data

Immunoprecipitation - Anti-ZPR9 antibody (AB174657)

Detection of ZPR9 by Western Blot of Immunoprecipitate.<br> Immunoprecipitation of HeLa whole cell lysate using ab174657 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane) followed by detection of ZPR9 with ab174657 at 0.1μg/ml (lane 2). The lysate in lanes 1 and 3 was precipiated with alternative anti-ZPR9 antibodies. The lysate in lane 4 is precipitated with an IgG control antibody.

All lanes:

Immunoprecipitation - Anti-ZPR9 antibody (ab174657)

Predicted band size: 54 kDa

true

Exposure time: 10s

Western blot - Anti-ZPR9 antibody (AB174657)
  • WB

Supplier Data

Western blot - Anti-ZPR9 antibody (AB174657)

All lanes:

Western blot - Anti-ZPR9 antibody (ab174657) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 54 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ZNF622 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q969S3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab174657 was affinity purified using an epitope specific to ZPR9 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZPR9 also known as Zinc Finger Protein of Unknown Function 9 is a nuclear protein with a molecular mass of approximately 60 kDa. It contains zinc finger domains which serve as key structural motifs for binding to DNA. ZPR9 is expressed in various tissues including liver and brain but shows a higher expression in immune cells. It functions through specific DNA interactions regulating the transcriptional activity of several genes involved in cell growth and immune response.
Biological function summary

ZPR9 plays a role in managing cellular processes that include transcriptional regulation and cellular signaling. It often associates with other proteins to form complexes that modulate gene expression in response to external stimuli. Research has shown that ZPR9 interacts with components of the transcription machinery influencing numerous biological functions that encompass immune system modulation and cell cycle progression.

Pathways

ZPR9 contributes significantly to the MAPK signaling pathway and NF-kB pathway. In the MAPK pathway it connects with proteins such as ERK1/2 impacting cellular responses like proliferation and differentiation. In the NF-kB pathway ZPR9 has links with protein p65 contributing to the regulation of immune responses and inflammation. These connections indicate its integration in key signaling networks vital for cellular homeostasis and immune regulation.

ZPR9 correlates with autoimmune diseases and cancer. Its abnormally high expression in conditions like rheumatoid arthritis suggests roles in inflammatory processes. Moreover alterations in ZPR9 expression have association with cancer development where it may impact tumor growth and metastasis. In autoimmune conditions it interacts with other proteins including NF-kB showing its influence on inflammatory pathways and potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pre-60S-associated cytoplasmic factor involved in the cytoplasmic maturation of the 60S subunit.
See full target information ZNF622
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:14975 PubMed36056100

2022

Retro-2 alters Golgi structure.

Applications

Unspecified application

Species

Unspecified reactive species

Xihua Yue,Bopil Gim,Lianhui Zhu,Chuanting Tan,Yi Qian,Morven Graham,Xinran Liu,Intaek Lee
View all publications
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Product promise

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