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AB262879

Anti-ZRANB1 antibody

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(1 Publication)

Rabbit Polyclonal ZRANB1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZRANB1 aa 550-700.

View Alternative Names

TRABID, ZRANB1, Ubiquitin thioesterase ZRANB1, TRAF-binding domain-containing protein, Zinc finger Ran-binding domain-containing protein 1, hTrabid

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ZRANB1 antibody (AB262879)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ZRANB1 antibody (AB262879)

PFA-fixed, Triton X-100 permeabilized human U-2OS cells stained for ZRANB1 (green) using ab262879 at 4 µg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZRANB1 antibody (AB262879)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZRANB1 antibody (AB262879)

Paraffin-embedded human ovary tissue stained for ZRANB1 using ab262879 at 1/200 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ZRANB1 aa 550-700. The exact immunogen used to generate this antibody is proprietary information.

Q9UGI0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZRANB1 also known as TRABID is a zinc finger RANBP2-type containing 1 protein with a mass of approximately 89 kDa. This protein functions majorly as a deubiquitinating enzyme specializing in cleaving Lys29- and Lys33-linked polyubiquitin chains. It is expressed across various tissues with notable expression in the human brain indicating its importance in neural functions. Mechanically ZRANB1 contains several zinc finger motifs which facilitate its interaction with ubiquitin substrates playing a critical role in protein ubiquitination homeostasis.
Biological function summary

ZRANB1 acts in the regulation of protein degradation and turnover impacting various cellular processes like signal transduction and cell cycle control. It functions as part of the ubiquitin-proteasome system which is a large multi-protein complex responsible for maintaining cellular protein homeostasis. Through its activity ZRANB1 influences not only regular cellular operations but also responds to stress signals by adjusting the degradation of proteins therefore impacting numerous physiological processes.

Pathways

ZRANB1 participates in the Wnt signaling pathway and the NF-kB pathway both of which are important for cell proliferation and immune response regulation. In the Wnt pathway it regulates the stability and signaling activity of key proteins like beta-catenin. Meanwhile ZRANB1's action in the NF-kB pathway involves modulating the degradation of ubiquitinated proteins with relations to proteins like NEMO. These pathways highlight the specific regulatory roles played by ZRANB1 within signaling networks.

ZRANB1 has been implicated in cancer and neurological disorders. In cancer aberrant regulation or mutations in ZRANB1 can lead to uncontrolled cell growth due to misregulation of the Wnt signaling pathway. In neurological disorders potential links to proteins involved in neural development highlight its role in maintaining neural homeostasis. By influencing pathways connected to these diseases ZRANB1 interacts with other proteins such as APC in cancer and possibly tau proteins in neurodegenerative disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ubiquitin thioesterase, which specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin (PubMed : 22157957, PubMed : 23827681, PubMed : 25752573, PubMed : 25752577). Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones (PubMed : 18281465). Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription (PubMed : 18281465). Acts as a regulator of autophagy by mediating deubiquitination of PIK3C3/VPS34, thereby promoting autophagosome maturation (PubMed : 33637724). Plays a role in the regulation of cell morphology and cytoskeletal organization (PubMed : 21834987). Required in the stress fiber dynamics and cell migration (PubMed : 21834987).
See full target information ZRANB1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:1810 PubMed37002234

2023

TRABID overexpression enables synthetic lethality to PARP inhibitor via prolonging 53BP1 retention at double-strand breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Ma,Yingke Zhou,Penglin Pan,Haixin Yu,Zixi Wang,Lei Lily Li,Bing Wang,Yuqian Yan,Yunqian Pan,Qi Ye,Tianjie Liu,Xiaoyu Feng,Shan Xu,Ke Wang,Xinyang Wang,Yanlin Jian,Bohan Ma,Yizeng Fan,Yang Gao,Haojie Huang,Lei Li
View all publications
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Product promise

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