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AB82132

Anti-ZRANB1 antibody

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(3 Publications)

Goat Polyclonal ZRANB1 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ZRANB1 aa 650-700.

View Alternative Names

TRABID, ZRANB1, Ubiquitin thioesterase ZRANB1, TRAF-binding domain-containing protein, Zinc finger Ran-binding domain-containing protein 1, hTrabid

1 Images
Western blot - Anti-ZRANB1 antibody (AB82132)
  • WB

Unknown

Western blot - Anti-ZRANB1 antibody (AB82132)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-ZRANB1 antibody (ab82132) at 0.1 µg/mL

All lanes:

Human Cerebellum Lysate (35ug in RIPA buffer)

Predicted band size: 81 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ZRANB1 aa 650-700. The exact immunogen used to generate this antibody is proprietary information.

Q9UGI0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZRANB1 also known as TRABID is a zinc finger RANBP2-type containing 1 protein with a mass of approximately 89 kDa. This protein functions majorly as a deubiquitinating enzyme specializing in cleaving Lys29- and Lys33-linked polyubiquitin chains. It is expressed across various tissues with notable expression in the human brain indicating its importance in neural functions. Mechanically ZRANB1 contains several zinc finger motifs which facilitate its interaction with ubiquitin substrates playing a critical role in protein ubiquitination homeostasis.
Biological function summary

ZRANB1 acts in the regulation of protein degradation and turnover impacting various cellular processes like signal transduction and cell cycle control. It functions as part of the ubiquitin-proteasome system which is a large multi-protein complex responsible for maintaining cellular protein homeostasis. Through its activity ZRANB1 influences not only regular cellular operations but also responds to stress signals by adjusting the degradation of proteins therefore impacting numerous physiological processes.

Pathways

ZRANB1 participates in the Wnt signaling pathway and the NF-kB pathway both of which are important for cell proliferation and immune response regulation. In the Wnt pathway it regulates the stability and signaling activity of key proteins like beta-catenin. Meanwhile ZRANB1's action in the NF-kB pathway involves modulating the degradation of ubiquitinated proteins with relations to proteins like NEMO. These pathways highlight the specific regulatory roles played by ZRANB1 within signaling networks.

ZRANB1 has been implicated in cancer and neurological disorders. In cancer aberrant regulation or mutations in ZRANB1 can lead to uncontrolled cell growth due to misregulation of the Wnt signaling pathway. In neurological disorders potential links to proteins involved in neural development highlight its role in maintaining neural homeostasis. By influencing pathways connected to these diseases ZRANB1 interacts with other proteins such as APC in cancer and possibly tau proteins in neurodegenerative disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ubiquitin thioesterase, which specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin (PubMed : 22157957, PubMed : 23827681, PubMed : 25752573, PubMed : 25752577). Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones (PubMed : 18281465). Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription (PubMed : 18281465). Acts as a regulator of autophagy by mediating deubiquitination of PIK3C3/VPS34, thereby promoting autophagosome maturation (PubMed : 33637724). Plays a role in the regulation of cell morphology and cytoskeletal organization (PubMed : 21834987). Required in the stress fiber dynamics and cell migration (PubMed : 21834987).
See full target information ZRANB1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of oncology 2022:7130634 PubMed35602293

2022

HLA-DQB1-AS1 Promotes Cell Proliferation, Inhibits Apoptosis, and Binds with ZRANB2 Protein in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwu Long,Longfei Liu,Xiaoyun Zhou,Xianzhou Lu,Lei Qin

American journal of cancer research 11:4807-4825 PubMed34765294

2021

Deubiquitinase ZRANB1 drives hepatocellular carcinoma progression through SP1-LOXL2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Li,Qing Chao,Yue Liu,Jiayu Fang,Jing Xie,Jing Zhen,Yongqi Ding,Bidong Fu,Yun Ke,Fan Xiao,Honghu Wu,Zhaoxia Huang,Haibin Hao,Da Huang

Journal of neurochemistry 115:1077-89 PubMed20854368

2010

Low-density lipoprotein receptor-related protein-1: a serial clearance homeostatic mechanism controlling Alzheimer's amyloid β-peptide elimination from the brain.

Applications

Unspecified application

Species

Unspecified reactive species

Berislav V Zlokovic,Rashid Deane,Abhay P Sagare,Robert D Bell,Ethan A Winkler
View all publications
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