JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB223062

Anti-ZRSR2 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal ZRSR2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZRSR2 aa 150-400.

View Alternative Names

U2AF1-RS2, U2AF1L2, U2AF1RS2, URP, ZRSR2, U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2, Renal carcinoma antigen NY-REN-20, U2(RNU2) small nuclear RNA auxiliary factor 1-like 2, U2AF35-related protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZRSR2 antibody (AB223062)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZRSR2 antibody (AB223062)

Paraffin embedded human testis tissue stained for ZRSR2 with ab223062 (1/100) in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ZRSR2 antibody (AB223062)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZRSR2 antibody (AB223062)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ZRSR2 (green) using ab223062 (1/100) in ICC/IF. Secondary : Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

Western blot - Anti-ZRSR2 antibody (AB223062)
  • WB

Supplier Data

Western blot - Anti-ZRSR2 antibody (AB223062)

All lanes:

Western blot - Anti-ZRSR2 antibody (ab223062) at 1/500 dilution

Lane 1:

K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate

Lane 2:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Lane 3:

mouse heart tissue lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG at 1/50000 dilution

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ZRSR2 aa 150-400. The exact immunogen used to generate this antibody is proprietary information.

Q15696

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZRSR2 (Zinc finger CCCH-type RNA-binding motif and serine/arginine-rich 2) plays a role in splicing influencing the splicing process of pre-mRNA by recognizing the 3' splice site. This protein weighs around 53 kDa and can be found in both the nucleus and cytoplasm of cells. Its expression occurs ubiquitously across various human tissues highlighting its foundational roles in cellular function.
Biological function summary

ZRSR2 is involved in regulating normal splicing via the recognition of U12-type introns. The protein is a part of the spliceosomal complex working closely with other spliceosomal components. This ensures accurate removal of these minor introns during the RNA processing phase. Mutations in ZRSR2 can lead to splicing errors which suggests its importance in gene expression.

Pathways

Splicing and gene expression are significant pathways involving ZRSR2. It is functionally connected to the spliceosomal complex and interacts with snRNP particles like U2AF2 and U2AF1. These interactions highlight ZRSR2's role as a critical player in post-transcriptional modification processes ensuring that genes are correctly expressed.

Malfunctions in ZRSR2 are linked to myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML). ZRSR2 mutations can result in an improper splicing of RNA contributing to these hematologic conditions. In the context of these diseases ZRSR2's interaction with related splicing factors such as SF3B1 further emphasizes the impact of defective splicing in the development and progression of these disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Pre-mRNA-binding protein required for splicing of both U2- and U12-type introns. Selectively interacts with the 3'-splice site of U2- and U12-type pre-mRNAs and promotes different steps in U2 and U12 intron splicing. Recruited to U12 pre-mRNAs in an ATP-dependent manner and is required for assembly of the prespliceosome, a precursor to other spliceosomal complexes. For U2-type introns, it is selectively and specifically required for the second step of splicing.
See full target information ZRSR2
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:5015-5033 PubMed40860180

2025

Citrus Pectin Supplementation Alleviated Hepatic Lipid Accumulation through Gut Microbiota Indole Lactic Acid Promoting Hepatic Bile Acid Synthesis and Excretion.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijun Pan,Xinyuan Jin,Qing Li,Yuqing Zhou,Yupeng Zeng,Xin Wang,Yufeng Jin,Yu Chen,Dan Li,Wenhua Ling
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB172634

Anti-SF3B1 antibody [EPR11986]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SF3B1 antibody [EPR11986] (AB172634)

  • 1
  • 1
Primary Antibodies

AB172614

Anti-U2AF35/U2AF1 antibody [EPR12649(B)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (AB172614)

  • 5
  • 1
Primary Antibodies

AB170105

Anti-RALY antibody [EPR10121]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RALY antibody [EPR10121] (AB170105)

  • 0
  • 0
Primary Antibodies

AB37530

Anti-U2AF65 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF65 antibody (AB37530)

  • 5
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com