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AB243442

Anti-ZSWIM8 antibody

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(1 Publication)

Rabbit Polyclonal ZSWIM8 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZSWIM8 aa 1-50.

View Alternative Names

KIAA0913, ZSWIM8, Zinc finger SWIM domain-containing protein 8

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ZSWIM8 antibody (AB243442)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZSWIM8 antibody (AB243442)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling NOL5A using ab242201 at 4 μg/ml (green) in ICC/IF. Nuclear probe is visualized in blue, microtubules in red.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZSWIM8 antibody (AB243442)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZSWIM8 antibody (AB243442)

Formalin-fixed, paraffin-embedded human rectum tissue stained for ZSWIM8 with ab243442 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ZSWIM8 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

A7E2V4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZSWIM8 also known as Zinc Finger SWIM-Type Containing 8 is an E3 ubiquitin ligase complex with an approximate mass of 180 kDa. This protein plays a role in the ubiquitination process which systemically adds ubiquitin to substrate proteins to mark them for degradation. ZSWIM8 is expressed in various tissues including the heart liver and skeletal muscle indicating its broad functional involvement within the human body.
Biological function summary

ZSWIM8 participates in the selective degradation of mRNA acting as a modifier within the mRNA decay pathway. It forms a complex with other proteins including ZNF598 and TRIP12 to mediate this process efficiently. By tagging specific RNA for degradation ZSWIM8 helps regulate gene expression and maintain cellular homeostasis. This activity implies its importance in cellular differentiation and response mechanisms under various physiological conditions.

Pathways

ZSWIM8 is significant in the nonsense-mediated decay (NMD) pathway which monitors mRNA for translation errors and prevents the accumulation of potentially harmful or non-functional proteins. This pathway involves cooperation with factors such as UPF1 and SMG5 that enhance the recognition and subsequent decay of flawed RNA transcripts. Through these interactions ZSWIM8 aids in preserving the integrity and accuracy of protein synthesis a critical task in cellular quality control mechanisms.

Defects in ZSWIM8 activity have associations with neurodevelopmental and muscular disorders. Disruption in the mRNA decay process can contribute to conditions such as intellectual disabilities and dystrophic pathologies. Its interaction with proteins like UPF1 and other decay-related factors highlights its potential involvement in these disorders. Studying the function and regulation of ZSWIM8 could offer insights into novel therapeutic strategies for managing these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex that promotes target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs) (PubMed : 33184234, PubMed : 33184237). The SCF-like E3 ubiquitin-protein ligase complex acts by catalyzing ubiquitination and subsequent degradation of AGO proteins (AGO1, AGO2, AGO3 and/or AGO4), thereby exposing miRNAs for degradation (PubMed : 33184234, PubMed : 33184237). Specifically recognizes and binds AGO proteins when they are engaged with a TDMD target (PubMed : 33184234). May also act as a regulator of axon guidance : specifically recognizes misfolded ROBO3 and promotes its ubiquitination and subsequent degradation (PubMed : 24012004). Plays an essential role for proper embryonic development of heart and lung (By similarity). Controls protein quality of DAB1, a key signal molecule for brain development, thus protecting its signaling strength. Mechanistically, recognizes intrinsically disordered regions of DAB1 and eliminates misfolded DAB1 that cannot be properly phosphorylated (By similarity).. (Microbial infection) Participates in Zika virus inhibition of IFN signaling by acting as a scaffold protein to connect ZSWIM8/CUL3 ligase complex and STAT2, leading to STAT2 degradation.
See full target information ZSWIM8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of cardiology 17:104832 PubMed40575431

2025

Hydrogen alleviates right ventricular hypertrophy by inhibiting ferroptosis restoration of the Nrf2/HO-1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Cai Bai,Hong-Xiao Yang,Cheng-Chuang Zhan,Lu-Qi Zhao,Jia-Ren Liu,Wei Yang
View all publications
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Product promise

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