Rabbit Polyclonal ZSWIM8 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZSWIM8 aa 1-50.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ZSWIM8 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link A7E2V4
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Cow | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow | Dilution info - | Notes - |
Target data
Function
Substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex that promotes target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs) (PubMed:33184234, PubMed:33184237). The SCF-like E3 ubiquitin-protein ligase complex acts by catalyzing ubiquitination and subsequent degradation of AGO proteins (AGO1, AGO2, AGO3 and/or AGO4), thereby exposing miRNAs for degradation (PubMed:33184234, PubMed:33184237). Specifically recognizes and binds AGO proteins when they are engaged with a TDMD target (PubMed:33184234). May also act as a regulator of axon guidance: specifically recognizes misfolded ROBO3 and promotes its ubiquitination and subsequent degradation (PubMed:24012004). Plays an essential role for proper embryonic development of heart and lung (By similarity). Controls protein quality of DAB1, a key signal molecule for brain development, thus protecting its signaling strength. Mechanistically, recognizes intrinsically disordered regions of DAB1 and eliminates misfolded DAB1 that cannot be properly phosphorylated (By similarity). (Microbial infection) Participates in Zika virus inhibition of IFN signaling by acting as a scaffold protein to connect ZSWIM8/CUL3 ligase complex and STAT2, leading to STAT2 degradation.
Alternative names
KIAA0913, ZSWIM8, Zinc finger SWIM domain-containing protein 8
Recommended products
Rabbit Polyclonal ZSWIM8 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZSWIM8 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ZSWIM8 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link A7E2V4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZSWIM8 also known as Zinc Finger SWIM-Type Containing 8 is an E3 ubiquitin ligase complex with an approximate mass of 180 kDa. This protein plays a role in the ubiquitination process which systemically adds ubiquitin to substrate proteins to mark them for degradation. ZSWIM8 is expressed in various tissues including the heart liver and skeletal muscle indicating its broad functional involvement within the human body.
- Biological function summary
ZSWIM8 participates in the selective degradation of mRNA acting as a modifier within the mRNA decay pathway. It forms a complex with other proteins including ZNF598 and TRIP12 to mediate this process efficiently. By tagging specific RNA for degradation ZSWIM8 helps regulate gene expression and maintain cellular homeostasis. This activity implies its importance in cellular differentiation and response mechanisms under various physiological conditions.
- Pathways
ZSWIM8 is significant in the nonsense-mediated decay (NMD) pathway which monitors mRNA for translation errors and prevents the accumulation of potentially harmful or non-functional proteins. This pathway involves cooperation with factors such as UPF1 and SMG5 that enhance the recognition and subsequent decay of flawed RNA transcripts. Through these interactions ZSWIM8 aids in preserving the integrity and accuracy of protein synthesis a critical task in cellular quality control mechanisms.
- Associated diseases and disorders
Defects in ZSWIM8 activity have associations with neurodevelopmental and muscular disorders. Disruption in the mRNA decay process can contribute to conditions such as intellectual disabilities and dystrophic pathologies. Its interaction with proteins like UPF1 and other decay-related factors highlights its potential involvement in these disorders. Studying the function and regulation of ZSWIM8 could offer insights into novel therapeutic strategies for managing these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-ZSWIM8 antibody (ab243442)
PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling NOL5A using Anti-NOL5A antibody [CL2603] ab242201 at 4 μg/ml (green) in ICC/IF. Nuclear probe is visualized in blue, microtubules in red.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZSWIM8 antibody (ab243442)
Formalin-fixed, paraffin-embedded human rectum tissue stained for ZSWIM8 with ab243442 at 1/50 dilution in immunohistochemical analysis.
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