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AB202898

Anti-ZWILCH antibody [EPR18035-28]

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(1 Publication)

Rabbit Recombinant Monoclonal ZWILCH antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Protein zwilch homolog, hZwilch, ZWILCH

4 Images
Immunoprecipitation - Anti-ZWILCH antibody [EPR18035-28] (AB202898)
  • IP

Supplier Data

Immunoprecipitation - Anti-ZWILCH antibody [EPR18035-28] (AB202898)

ZWILCH was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab202898 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab202898 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : HeLa whole cell lysate 10ug (Input). Lane 2 : ab202898 IP in HeLa whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab202898 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-ZWILCH antibody [EPR18035-28] (ab202898)

Predicted band size: 67 kDa

false

Western blot - Anti-ZWILCH antibody [EPR18035-28] (AB202898)
  • WB

Supplier Data

Western blot - Anti-ZWILCH antibody [EPR18035-28] (AB202898)

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ZWILCH antibody [EPR18035-28] (ab202898) at 1/2000 dilution

Lane 1:

HEK-293 (Human epithelial cells from embryonic kidney) cell lysate at 20 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

Lane 3:

PC-3 (Human prostate adenocarcinoma cell line) cell lysate at 20 µg

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 67 kDa

Observed band size: 67 kDa

false

Exposure time: 15s

Western blot - Anti-ZWILCH antibody [EPR18035-28] (AB202898)
  • WB

Supplier Data

Western blot - Anti-ZWILCH antibody [EPR18035-28] (AB202898)

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ZWILCH antibody [EPR18035-28] (ab202898) at 1/2000 dilution

All lanes:

Mouse brain tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 67 kDa

Observed band size: 67 kDa

false

Exposure time: 3min

Western blot - Anti-ZWILCH antibody [EPR18035-28] (AB202898)
  • WB

Supplier Data

Western blot - Anti-ZWILCH antibody [EPR18035-28] (AB202898)

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ZWILCH antibody [EPR18035-28] (ab202898) at 1/2000 dilution

Lane 1:

C6 (Rat glial tumor cells) cell lysate at 10 µg

Lane 2:

PC-12 (Rat adrenal gland pheochromocytoma) cell lysate at 10 µg

Lane 3:

NIH/3T3 (Mouse embryo fibroblast cells) cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 67 kDa

Observed band size: 67 kDa

false

Exposure time: 15s

  • Carrier free

    Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18035-28

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZWILCH also known as ZW10 interactor-like or ZWILCH kinetochore protein is an important component in cell division especially during mitosis. The protein has a molecular mass of approximately 85 kDa. ZWILCH is highly expressed in dividing cells with enrichment in tissues that have high mitotic rates like the bone marrow and germinal centers. Functionally ZWILCH is a component of the RZZ complex comprising ROD (also known as KNTC1) and ZW10 helping in the proper attachment of chromosomes at the kinetochores.
Biological function summary

ZWILCH is fundamental in ensuring accurate chromosome alignment and segregation during cell division. It plays a part as a component of the RZZ complex. The RZZ complex localizes to kinetochores which are critical for connecting spindle microtubules to chromosomes promoting bioriented chromosome alignment. Moreover ZWILCH contributes to the spindle assembly checkpoint a safety mechanism that prevents chromosome mis-segregation by delaying progress to anaphase until all chromosomes are correctly attached.

Pathways

ZWILCH plays integral roles in the mitotic spindle checkpoint and chromosomal stability pathways. Within these pathways ZWILCH associates closely with spindle assembly checkpoint proteins such as MAD2 and BUBR1 which monitor kinetochore-microtubule attachment. ZWILCH through its role in the RZZ complex communicates tension status between sister chromatids ensuring proper cell-cycle progression and genomic integrity.

ZWILCH has significant connections to both cancer and aneuploidy. Misregulation or mutations in ZWILCH can result in chromosomal instability a main feature in various cancers. Aberrant activity of the RZZ complex where ZWILCH is vital could disrupt normal cell division and cause aneuploidy a condition characterized by an abnormal number of chromosomes seen in numerous cancer types and genetic disorders. Therefore ZWILCH and its associations with proteins like ZW10 and ROD serve as potential biomarkers for chromosomal instability-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed : 15824131).
See full target information ZWILCH
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature structural & molecular biology 32:2060-2075 PubMed40527988

2025

DMXL1 promotes recruitment of V1-ATPase to lysosomes upon TRPML1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Chan Lee,Matthew J G Eldridge,Miguel A Gonzalez-Lozano,Thomas Bresnahan,Zachary Niday,Donato Del Camino,Tao Fu,Joao A Paulo,Magdalene M Moran,Sophie Helaine,J Wade Harper
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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