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AB251386

Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free

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Rabbit Recombinant Monoclonal ZWILCH antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

Protein zwilch homolog, hZwilch, ZWILCH

4 Images
Immunoprecipitation - Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free (AB251386)
  • IP

Supplier Data

Immunoprecipitation - Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free (AB251386)

This data was developed using ab202898, the same antibody clone in a different buffer formulation.

ZWILCH was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab202898 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab202898 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : HeLa whole cell lysate 10ug (Input). Lane 2 : ab202898 IP in HeLa whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab202898 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-ZWILCH antibody [EPR18035-28] (<a href='/en-us/products/primary-antibodies/zwilch-antibody-epr18035-28-ab202898'>ab202898</a>)

Predicted band size: 67 kDa

false

Western blot - Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free (AB251386)
  • WB

Supplier Data

Western blot - Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free (AB251386)

This data was developed using ab202898, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ZWILCH antibody [EPR18035-28] (<a href='/en-us/products/primary-antibodies/zwilch-antibody-epr18035-28-ab202898'>ab202898</a>) at 1/2000 dilution

Lane 1:

HEK-293 (Human epithelial cells from embryonic kidney) cell lysate at 20 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

Lane 3:

PC-3 (Human prostate adenocarcinoma cell line) cell lysate at 20 µg

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 67 kDa

Observed band size: 67 kDa

false

Exposure time: 15s

Western blot - Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free (AB251386)
  • WB

Supplier Data

Western blot - Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free (AB251386)

This data was developed using ab202898, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ZWILCH antibody [EPR18035-28] (<a href='/en-us/products/primary-antibodies/zwilch-antibody-epr18035-28-ab202898'>ab202898</a>) at 1/2000 dilution

All lanes:

Mouse brain tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 67 kDa

Observed band size: 67 kDa

false

Exposure time: 3min

Western blot - Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free (AB251386)
  • WB

Supplier Data

Western blot - Anti-ZWILCH antibody [EPR18035-28] - BSA and Azide free (AB251386)

This data was developed using ab202898, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ZWILCH antibody [EPR18035-28] (<a href='/en-us/products/primary-antibodies/zwilch-antibody-epr18035-28-ab202898'>ab202898</a>) at 1/2000 dilution

Lane 1:

C6 (Rat glial tumor cells) cell lysate at 10 µg

Lane 2:

PC-12 (Rat adrenal gland pheochromocytoma) cell lysate at 10 µg

Lane 3:

NIH/3T3 (Mouse embryo fibroblast cells) cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 67 kDa

Observed band size: 67 kDa

false

Exposure time: 15s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18035-28

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251386 is the carrier-free version of ab202898.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZWILCH also known as ZW10 interactor-like or ZWILCH kinetochore protein is an important component in cell division especially during mitosis. The protein has a molecular mass of approximately 85 kDa. ZWILCH is highly expressed in dividing cells with enrichment in tissues that have high mitotic rates like the bone marrow and germinal centers. Functionally ZWILCH is a component of the RZZ complex comprising ROD (also known as KNTC1) and ZW10 helping in the proper attachment of chromosomes at the kinetochores.
Biological function summary

ZWILCH is fundamental in ensuring accurate chromosome alignment and segregation during cell division. It plays a part as a component of the RZZ complex. The RZZ complex localizes to kinetochores which are critical for connecting spindle microtubules to chromosomes promoting bioriented chromosome alignment. Moreover ZWILCH contributes to the spindle assembly checkpoint a safety mechanism that prevents chromosome mis-segregation by delaying progress to anaphase until all chromosomes are correctly attached.

Pathways

ZWILCH plays integral roles in the mitotic spindle checkpoint and chromosomal stability pathways. Within these pathways ZWILCH associates closely with spindle assembly checkpoint proteins such as MAD2 and BUBR1 which monitor kinetochore-microtubule attachment. ZWILCH through its role in the RZZ complex communicates tension status between sister chromatids ensuring proper cell-cycle progression and genomic integrity.

ZWILCH has significant connections to both cancer and aneuploidy. Misregulation or mutations in ZWILCH can result in chromosomal instability a main feature in various cancers. Aberrant activity of the RZZ complex where ZWILCH is vital could disrupt normal cell division and cause aneuploidy a condition characterized by an abnormal number of chromosomes seen in numerous cancer types and genetic disorders. Therefore ZWILCH and its associations with proteins like ZW10 and ROD serve as potential biomarkers for chromosomal instability-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed : 15824131).
See full target information ZWILCH
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com