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AB168501

Anti-ZWINT antibody

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(1 Publication)

Mouse Polyclonal ZWINT antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ZWINT.

View Alternative Names

Outer kinetochore KNL1 complex subunit ZWINT, ZW10 interactor, ZW10-interacting protein 1, Zwint-1, ZWINT

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ZWINT antibody (AB168501)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ZWINT antibody (AB168501)

Immunofluorescent analysis of HeLa cells labeling ZWINT with ab168501 at 10 µg/ml.

Western blot - Anti-ZWINT antibody (AB168501)
  • WB

Unknown

Western blot - Anti-ZWINT antibody (AB168501)

All lanes:

Western blot - Anti-ZWINT antibody (ab168501) at 1 µg/mL

All lanes:

Human liver lysate at 50 µg

Predicted band size: 31 kDa

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Western blot - Anti-ZWINT antibody (AB168501)
  • WB

Unknown

Western blot - Anti-ZWINT antibody (AB168501)

All lanes:

Western blot - Anti-ZWINT antibody (ab168501) at 1 µg/mL

Lane 1:

ZWINT transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Predicted band size: 31 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ZWINT.

O95229

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZWINT also known as ZW10 interactor is a protein that plays an important role in the attachment of kinetochores to the spindle microtubules during mitosis. It has a molecular weight of approximately 35 kDa. This protein is broadly expressed in various tissues with especially high presence in dividing cells reflecting its importance in cell division processes.
Biological function summary

ZWINT functions as a part of the kinetochore complex which is essential for chromosome segregation during mitotic cell division. It interacts with other kinetochore proteins to facilitate the correct attachment of chromosomes to the spindle apparatus. This interaction ensures that chromosomes are accurately distributed to daughter cells maintaining genomic stability.

Pathways

The ZWINT protein is integral in the mitotic spindle checkpoint pathway also known as the spindle assembly checkpoint. It works closely with proteins such as ZW10 and Rod to monitor and regulate the attachment of spindle microtubules to the kinetochores preventing aneuploidy during cell division. This pathway ensures that mitosis does not proceed until all chromosomes are properly aligned and attached contributing to the fidelity of cell division.

Mutations or dysregulation in ZWINT have been linked to certain cancers due to its influence on chromosome segregation. Disruptions in ZWINT function might also contribute to chromosomal instability which can lead to tumorigenesis. In cancerous contexts proteins such as Mad2 and BubR1 which are part of the spindle assembly checkpoint pathway also show altered interactions highlighting a complex network of regulatory proteins involved in maintaining cellular homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions (PubMed : 15094189, PubMed : 15485811, PubMed : 15824131, PubMed : 16732327, PubMed : 24530301, PubMed : 27881301, PubMed : 38459127, PubMed : 38459128). Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules (PubMed : 15094189, PubMed : 15485811, PubMed : 16732327). The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle (PubMed : 15094189, PubMed : 15485811, PubMed : 15824131, PubMed : 16732327, PubMed : 24530301, PubMed : 38459127, PubMed : 38459128). Targets the RZZ complex to the kinetochore at prometaphase (PubMed : 15485811). Recruits MAD2L1 to the kinetochore, but is not required for BUB1B localization (By similarity). In addition to orienting mitotic chromosomes, it is also essential for alignment of homologous chromosomes during meiotic metaphase I (By similarity). In meiosis I, required to activate the spindle assembly checkpoint at unattached kinetochores to correct erroneous kinetochore-microtubule attachments (PubMed : 15485811).
See full target information ZWINT
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 11:e0163328 PubMed27681508

2016

Alternative Splice Variants Modulates Dominant-Negative Function of Helios in T-Cell Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Shaorong Zhao,Wei Liu,Yinghui Li,Pengjiang Liu,Shufang Li,Daolei Dou,Yue Wang,Rongcun Yang,Rong Xiang,Feifei Liu
View all publications
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