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AB71982

Anti-ZWINT antibody

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(4 Publications)

Rabbit Polyclonal ZWINT antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ZWINT.

View Alternative Names

Outer kinetochore KNL1 complex subunit ZWINT, ZW10 interactor, ZW10-interacting protein 1, Zwint-1, ZWINT

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ZWINT antibody (AB71982)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ZWINT antibody (AB71982)

Immunocytochemistry/Immunofluorescence analysis of formaldelyde-fixed HeLa cells labelling ZWINT with ab71982 at at 1/200 (1µg/ml). A FITC-conjugated goat anti-rabbit IgG (1/100) was used as the secondary antibody.

Immunoprecipitation - Anti-ZWINT antibody (AB71982)
  • IP

Unknown

Immunoprecipitation - Anti-ZWINT antibody (AB71982)

Detection of ZWINT by Western Blot of Immunprecipitate.
ab71982 at 1µg/ml staining ZWINT in HeLa whole cell lysate immunoprecipitated using ab71982 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-ZWINT antibody (ab71982)

Predicted band size: 31 kDa

false

Western blot - Anti-ZWINT antibody (AB71982)
  • WB

Unknown

Western blot - Anti-ZWINT antibody (AB71982)

All lanes:

Western blot - Anti-ZWINT antibody (ab71982) at 0.04 µg

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 31 kDa

Observed band size: 28 kDa,38 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP, ICC/IF

applications

Immunogen

Synthetic Peptide within Human ZWINT. The exact immunogen used to generate this antibody is proprietary information.

O95229

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/250", "ICCIF-species-notes": "<p>Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab71982 was affinity purified using an epitope specific to ZWINT immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZWINT also known as ZW10 interactor is a protein that plays an important role in the attachment of kinetochores to the spindle microtubules during mitosis. It has a molecular weight of approximately 35 kDa. This protein is broadly expressed in various tissues with especially high presence in dividing cells reflecting its importance in cell division processes.
Biological function summary

ZWINT functions as a part of the kinetochore complex which is essential for chromosome segregation during mitotic cell division. It interacts with other kinetochore proteins to facilitate the correct attachment of chromosomes to the spindle apparatus. This interaction ensures that chromosomes are accurately distributed to daughter cells maintaining genomic stability.

Pathways

The ZWINT protein is integral in the mitotic spindle checkpoint pathway also known as the spindle assembly checkpoint. It works closely with proteins such as ZW10 and Rod to monitor and regulate the attachment of spindle microtubules to the kinetochores preventing aneuploidy during cell division. This pathway ensures that mitosis does not proceed until all chromosomes are properly aligned and attached contributing to the fidelity of cell division.

Mutations or dysregulation in ZWINT have been linked to certain cancers due to its influence on chromosome segregation. Disruptions in ZWINT function might also contribute to chromosomal instability which can lead to tumorigenesis. In cancerous contexts proteins such as Mad2 and BubR1 which are part of the spindle assembly checkpoint pathway also show altered interactions highlighting a complex network of regulatory proteins involved in maintaining cellular homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions (PubMed : 15094189, PubMed : 15485811, PubMed : 15824131, PubMed : 16732327, PubMed : 24530301, PubMed : 27881301, PubMed : 38459127, PubMed : 38459128). Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules (PubMed : 15094189, PubMed : 15485811, PubMed : 16732327). The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle (PubMed : 15094189, PubMed : 15485811, PubMed : 15824131, PubMed : 16732327, PubMed : 24530301, PubMed : 38459127, PubMed : 38459128). Targets the RZZ complex to the kinetochore at prometaphase (PubMed : 15485811). Recruits MAD2L1 to the kinetochore, but is not required for BUB1B localization (By similarity). In addition to orienting mitotic chromosomes, it is also essential for alignment of homologous chromosomes during meiotic metaphase I (By similarity). In meiosis I, required to activate the spindle assembly checkpoint at unattached kinetochores to correct erroneous kinetochore-microtubule attachments (PubMed : 15485811).
See full target information ZWINT
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 223: PubMed37934467

2023

A farnesyl-dependent structural role for CENP-E in expansion of the fibrous corona.

Applications

Unspecified application

Species

Unspecified reactive species

Jingchao Wu,Maximilian W D Raas,Paula Sobrevals Alcaraz,Harmjan R Vos,Eelco C Tromer,Berend Snel,Geert J P L Kops

Experimental and therapeutic medicine 22:818 PubMed34131441

2021

Zwint facilitates melanoma progression by promoting c-Myc expression.

Applications

Unspecified application

Species

Unspecified reactive species

Kuanhou Mou,Jian Zhang,Xin Mu,Lijuan Wang,Wenli Liu,Rui Ge

OncoTargets and therapy 12:10057-10075 PubMed31819501

2019

Integrated Analysis To Identify Molecular Biomarkers Of High-Grade Serous Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Manfei Si,Junji Zhang,Jianzhong Cao,Zhibo Xie,Shan Shu,Yapei Zhu,Jinghe Lang

Neuro-oncology 12:453-65 PubMed20406896

2010

Human umbilical cord blood stem cells show PDGF-D-dependent glioma cell tropism in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher S Gondi,Krishna Kumar Veeravalli,Bharathi Gorantla,Dzung H Dinh,Dan Fassett,Jeffrey D Klopfenstein,Meena Gujrati,Jasti S Rao
View all publications
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