AB58210

Anti-Zyxin antibody [2D1]

Name: Anti-Zyxin antibody [2D1] (ab58210) | Abcam
Brand: Abcam Limited
SKU: AB58210
Price: 715 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(9 Publications)

Mouse Monoclonal Zyxin antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ZYX.

View Alternative Names

Zyxin, Zyxin-2, ZYX

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Zyxin antibody [2D1] (AB58210)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Zyxin antibody [2D1] (AB58210)

ICC/IF image of ab58210 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58210, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

This image was generated using the ascites version of the product.

Flow Cytometry - Anti-Zyxin antibody [2D1] (AB58210)

Flow Cyt

Unknown

Flow Cytometry - Anti-Zyxin antibody [2D1] (AB58210)

Overlay histogram showing HeLa cells stained with ab58210 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58210, 0.5μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2D1

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ZYX.

Q15942

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 30^th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification notes

Purified from TCS.

Storage buffer

pH: 7.4

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Zyxin also known as ZYX is a mechanical regulator associated with cell adhesion and signaling. This protein has a molecular mass of approximately 61 kDa and shows expression in various cell types including those in the heart liver and lungs. As a component of focal adhesions zyxin plays a role in cytoskeletal dynamics by interacting with actin filaments and facilitating the formation of stress fibers.

Biological function summary

The protein has important roles in cellular processes such as migration and morphology. Zyxin often forms part of a larger protein complex that includes VASP and α-actinin which contribute to cell scaffolding and structural integrity. It serves as an organizer for cytoskeletal proteins ensuring proper cell movement and adaptability to cellular stress. Its actin-binding capacity enables it to regulate structural changes necessary for cellular adaptation to environmental stimuli.

Pathways

This protein is essential in the regulation of actin cytoskeleton associated pathways and adherens junction signaling. Zyxin interacts with Ena/VASP proteins which are significant in actin filament elongation helping regulate dynamic changes in cell shape and motility. Its relationship with LIM domain-containing proteins positions zyxin within pathways related to cell proliferation and signal transduction.

Zyxin has been implicated in cancer progression especially in metastasis where altered cell adhesion and movement play a role. It also relates to cardiovascular diseases due to its involvement in mechanotransduction pathways critical to heart muscle function. In cancer zyxin influences the activity of YAP/TAZ transcription regulators affecting tumor cell behavior. In cardiovascular scenarios the protein interacts with paxillin to mediate responses to mechanical load changes impacting heart disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity).

See full target information ZYX

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

NPJ microgravity 11:10 PubMed40090965

2025

The mechanosensitive channel ELKIN1 regulates cellular adaptations to simulated microgravity.

Applications

Unspecified application

Species

Unspecified reactive species

Daphne Wang,Giulia Silvani,Lioba Schroeter,Remi Brynn,Joshua Chou,Kate Poole

Nature communications 12:2226 PubMed33850145

2021

EpCAM promotes endosomal modulation of the cortical RhoA zone for epithelial organization.

Applications

Unspecified application

Species

Unspecified reactive species

Cécile Gaston,Simon De Beco,Bryant Doss,Meng Pan,Estelle Gauquelin,Joseph D'Alessandro,Chwee Teck Lim,Benoit Ladoux,Delphine Delacour

Communications biology 3:576 PubMed33067523

2020

Actomyosin and the MRTF-SRF pathway downregulate FGFR1 in mesenchymal stromal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jip Zonderland,Silvia Rezzola,Lorenzo Moroni

Stem cells (Dayton, Ohio) 38:948-959 PubMed32379914

2020

Mechanosensitive regulation of stanniocalcin-1 by zyxin and actin-myosin in human mesenchymal stromal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jip Zonderland,David B Gomes,Yves Pallada,Ivan L Moldero,Sandra Camarero-Espinosa,Lorenzo Moroni

Biomaterials 240:119854 PubMed32087459

2020

Dimensionality changes actin network through lamin A/C and zyxin.

Applications

Unspecified application

Species

Unspecified reactive species

Jip Zonderland,Ivan Lorenzo Moldero,Shivesh Anand,Carlos Mota,Lorenzo Moroni

Cellular signalling 63:109382 PubMed31376525

2019

On the relationship of YAP and FAK in hMSCs and osteosarcoma cells: Discrimination of FAK modulation by nuclear YAP depletion or YAP silencing.

Applications

Unspecified application

Species

Unspecified reactive species

Ayman Husari,Thorsten Steinberg,Martin Philipp Dieterle,Oswald Prucker,Jürgen Rühe,Britta Jung,Pascal Tomakidi

Matrix biology : journal of the International Society for Matrix Biology 85-86:112-127 PubMed31189077

2019

Transcriptomic analysis reveals that BMP4 sensitizes glioblastoma tumor-initiating cells to mechanical cues.

Applications

Unspecified application

Species

Unspecified reactive species

Jasmine H Hughes,Jeanette M Ewy,Joseph Chen,Sophie Y Wong,Kevin M Tharp,Andreas Stahl,Sanjay Kumar

ACS biomaterials science & engineering 5:3828-3842 PubMed33438423

2019

Molecular Organization of Integrin-Based Adhesion Complexes in Mouse Embryonic Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shumin Xia,Evelyn K F Yim,Pakorn Kanchanawong

Biochimica et biophysica acta 1853:388-95 PubMed25450971

2014

Imatinib restores VASP activity and its interaction with Zyxin in BCR-ABL leukemic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa A Bernusso,João A Machado-Neto,Fernando V Pericole,Karla P Vieira,Adriana S S Duarte,Fabiola Traina,Marc D Hansen,Sara T Olalla Saad,Karin S A Barcellos

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Zyxin antibody [2D1]

Immunocytochemistry/ Immunofluorescence - Anti-Zyxin antibody [2D1] (AB58210)

Flow Cytometry - Anti-Zyxin antibody [2D1] (AB58210)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise