Mouse Monoclonal Zyxin antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ZYX.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Zyxin antibody [2D1] (AB58210), expandable thumbnail](https://content.abcam.com/products/images/zyxin-antibody-2d1-ab58210--immunocytochemistry-immunofluorescence-img44324.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Zyxin antibody [2D1] (AB58210)")
![Flow Cytometry - Anti-Zyxin antibody [2D1] (AB58210), expandable thumbnail](https://content.abcam.com/products/images/zyxin-antibody-2d1-ab58210--flow-cytometry-img98168.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Zyxin antibody [2D1] (AB58210)")
Publications
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- Nature communications 12:22262021EpCAM promotes endosomal modulation of the cortical RhoA zone for epithelial organization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCécile Gaston,Simon De Beco,Bryant Doss,Meng Pan,Estelle Gauquelin,Joseph D'Alessandro,Chwee Teck Lim,Benoit Ladoux,Delphine DelacourPubMed 33850145
- Communications biology 3:5762020Actomyosin and the MRTF-SRF pathway downregulate FGFR1 in mesenchymal stromal cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJip Zonderland,Silvia Rezzola,Lorenzo MoroniPubMed 33067523
- Stem cells (Dayton, Ohio) 38:948-9592020Mechanosensitive regulation of stanniocalcin-1 by zyxin and actin-myosin in human mesenchymal stromal cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJip Zonderland et. al.PubMed 32379914
- Biomaterials 240:1198542020Dimensionality changes actin network through lamin A/C and zyxin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJip Zonderland et. al.PubMed 32087459
- Cellular signalling 63:1093822019On the relationship of YAP and FAK in hMSCs and osteosarcoma cells: Discrimination of FAK modulation by nuclear YAP depletion or YAP silencing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAyman Husari et. al.PubMed 31376525
- Matrix biology : journal of the International Society for Matrix Biology 85-86:112-1272019Transcriptomic analysis reveals that BMP4 sensitizes glioblastoma tumor-initiating cells to mechanical cues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJasmine H Hughes,Jeanette M Ewy,Joseph Chen,Sophie Y Wong,Kevin M Tharp,Andreas Stahl,Sanjay KumarPubMed 31189077
- ACS biomaterials science & engineering 5:3828-38422019Molecular Organization of Integrin-Based Adhesion Complexes in Mouse Embryonic Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShumin Xia,Evelyn K F Yim,Pakorn KanchanawongPubMed 33438423
- Biochimica et biophysica acta 1853:388-952014Imatinib restores VASP activity and its interaction with Zyxin in BCR-ABL leukemic cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVanessa A Bernusso,João A Machado-Neto,Fernando V Pericole,Karla P Vieira,Adriana S S Duarte,Fabiola Traina,Marc D Hansen,Sara T Olalla Saad,Karin S A BarcellosPubMed 25450971
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human ZYX. Database link Q15942
Reactivity data
Select an application| Flow Cyt | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity).
Alternative names
Zyxin, Zyxin-2, ZYX
Recommended products
Mouse Monoclonal Zyxin antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ZYX.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human ZYX. Database link Q15942
- Clone number
- 2D1
- Purity
- Tissue culture supernatant
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 30th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Zyxin also known as ZYX is a mechanical regulator associated with cell adhesion and signaling. This protein has a molecular mass of approximately 61 kDa and shows expression in various cell types including those in the heart liver and lungs. As a component of focal adhesions zyxin plays a role in cytoskeletal dynamics by interacting with actin filaments and facilitating the formation of stress fibers.
- Biological function summary
The protein has important roles in cellular processes such as migration and morphology. Zyxin often forms part of a larger protein complex that includes VASP and α-actinin which contribute to cell scaffolding and structural integrity. It serves as an organizer for cytoskeletal proteins ensuring proper cell movement and adaptability to cellular stress. Its actin-binding capacity enables it to regulate structural changes necessary for cellular adaptation to environmental stimuli.
- Pathways
This protein is essential in the regulation of actin cytoskeleton associated pathways and adherens junction signaling. Zyxin interacts with Ena/VASP proteins which are significant in actin filament elongation helping regulate dynamic changes in cell shape and motility. Its relationship with LIM domain-containing proteins positions zyxin within pathways related to cell proliferation and signal transduction.
- Associated diseases and disorders
Zyxin has been implicated in cancer progression especially in metastasis where altered cell adhesion and movement play a role. It also relates to cardiovascular diseases due to its involvement in mechanotransduction pathways critical to heart muscle function. In cancer zyxin influences the activity of YAP/TAZ transcription regulators affecting tumor cell behavior. In cardiovascular scenarios the protein interacts with paxillin to mediate responses to mechanical load changes impacting heart disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Zyxin antibody [2D1] (ab58210), expandable thumbnail](https://content.abcam.com/products/images/zyxin-antibody-2d1-ab58210--immunocytochemistry-immunofluorescence-img44324.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Zyxin antibody [2D1] (ab58210)")
Immunocytochemistry/ Immunofluorescence - Anti-Zyxin antibody [2D1] (ab58210)
ICC/IF image of ab58210 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58210, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
This image was generated using the ascites version of the product.
![Flow Cytometry - Anti-Zyxin antibody [2D1] (ab58210), expandable thumbnail](https://content.abcam.com/products/images/zyxin-antibody-2d1-ab58210--flow-cytometry-img98168.jpg "Flow Cytometry - Anti-Zyxin antibody [2D1] (ab58210)")
Flow Cytometry - Anti-Zyxin antibody [2D1] (ab58210)
Overlay histogram showing HeLa cells stained with ab58210 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58210, 0.5μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This image was generated using the ascites version of the product.
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