AB244277

Anti-Zyxin antibody

Name: Anti-Zyxin antibody (ab244277) | Abcam
Brand: Abcam Limited
SKU: AB244277
Price: 630 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Zyxin antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZYX aa 250-400.

View Alternative Names

Zyxin, Zyxin-2, ZYX

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zyxin antibody (AB244277)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zyxin antibody (AB244277)

Formalin-fixed, paraffin-embedded human spleen tissue stained for Zyxin using ab244277 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Zyxin antibody (AB244277)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Zyxin antibody (AB244277)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Zyxin (green) using ab244277 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ZYX aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

Q15942

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Zyxin also known as ZYX is a mechanical regulator associated with cell adhesion and signaling. This protein has a molecular mass of approximately 61 kDa and shows expression in various cell types including those in the heart liver and lungs. As a component of focal adhesions zyxin plays a role in cytoskeletal dynamics by interacting with actin filaments and facilitating the formation of stress fibers.

Biological function summary

The protein has important roles in cellular processes such as migration and morphology. Zyxin often forms part of a larger protein complex that includes VASP and α-actinin which contribute to cell scaffolding and structural integrity. It serves as an organizer for cytoskeletal proteins ensuring proper cell movement and adaptability to cellular stress. Its actin-binding capacity enables it to regulate structural changes necessary for cellular adaptation to environmental stimuli.

Pathways

This protein is essential in the regulation of actin cytoskeleton associated pathways and adherens junction signaling. Zyxin interacts with Ena/VASP proteins which are significant in actin filament elongation helping regulate dynamic changes in cell shape and motility. Its relationship with LIM domain-containing proteins positions zyxin within pathways related to cell proliferation and signal transduction.

Zyxin has been implicated in cancer progression especially in metastasis where altered cell adhesion and movement play a role. It also relates to cardiovascular diseases due to its involvement in mechanotransduction pathways critical to heart muscle function. In cancer zyxin influences the activity of YAP/TAZ transcription regulators affecting tumor cell behavior. In cardiovascular scenarios the protein interacts with paxillin to mediate responses to mechanical load changes impacting heart disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity).

See full target information ZYX

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:114309 PubMed38848215

2024

Lineage specification in glioblastoma is regulated by METTL7B.

Applications

Unspecified application

Species

Unspecified reactive species

Myrianni Constantinou,James Nicholson,Xinyu Zhang,Eleni Maniati,Sara Lucchini,Gabriel Rosser,Claire Vinel,Jun Wang,Yau Mun Lim,Sebastian Brandner,Sven Nelander,Sara Badodi,Silvia Marino

View all publications

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