Mouse Monoclonal Zyxin antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human ZYX aa 100-150 conjugated to Keyhole Limpet Haemocyanin.
Images
![Western blot - Anti-Zyxin antibody [ZOL301] (AB50391), expandable thumbnail](https://content.abcam.com/products/images/zyxin-antibody-zol301-ab50391--western-blot-img166849.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Zyxin antibody [ZOL301] (AB50391)")
![Western blot - Anti-Zyxin antibody [ZOL301] (AB50391), expandable thumbnail](https://content.abcam.com/products/images/zyxin-antibody-zol301-ab50391--western-blot-img167813.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Zyxin antibody [ZOL301] (AB50391)")
Publications
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- Reports of biochemistry & molecular biology 11:262-2692022Kinetic Changes of Ptdins (3,4,5) P3 within Fast and Slow Turnover Rates of Focal Adhesion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDhurgham Al-Fahad,Firas Alyaseen,Ahmed Al-Amery,Gagandeep Singh,Mote Srinath,Hafiz Muzzammel Rehman,Yahya AbbasPubMed 36164635
- iScience 25:1041232022Canonical Wnt signaling induces focal adhesion and Integrin beta-1 endocytosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNydia Tejeda-Muñoz,Marco Morselli,Yuki Moriyama,Pooja Sheladiya,Matteo Pellegrini,Edward M De RobertisPubMed 35402867
- The Journal of cell biology 220:2021Rab40-Cullin5 complex regulates EPLIN and actin cytoskeleton dynamics during cell migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErik S Linklater,Emily D Duncan,Ke-Jun Han,Algirdas Kaupinis,Mindaugas Valius,Traci R Lyons,Rytis PrekerisPubMed 33999101
- Turkish journal of biology = Turk biyoloji dergisi 44:381-3922020PtdIns(4,5)P2 and PtdIns(3,4,5)P3 dynamics during focal adhesions assembly and disassembly in a cancer cell line.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDhurgham Alfahad et. al.PubMed 33402865
- Journal of experimental & clinical cancer research 37:2212018Endothelial cells promote metastasis of prostate cancer by enhancing autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuizhe Zhao et. al.PubMed 30200999
- Nature communications 8:146392017Zyxin regulates endothelial von Willebrand factor secretion by reorganizing actin filaments around exocytic granules.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaofan Han et. al.PubMed 28256511
- Biomaterials 33:4998-50032012Nanotopography as modulator of human mesenchymal stem cell function.
- PloS one 7:e314232012Differential regulation of adhesion complex turnover by ROCK1 and ROCK2.
- ASN neuro 1:2009The neurogenic basic helix-loop-helix transcription factor NeuroD6 concomitantly increases mitochondrial mass and regulates cytoskeletal organization in the early stages of neuronal differentiation.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human ZYX aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q15942
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Cow | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity).
Alternative names
Zyxin, Zyxin-2, ZYX
Recommended products
Mouse Monoclonal Zyxin antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human ZYX aa 100-150 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human ZYX aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q15942
- Clone number
- ZOL301
- Purity
- Tissue culture supernatant
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Zyxin also known as ZYX is a mechanical regulator associated with cell adhesion and signaling. This protein has a molecular mass of approximately 61 kDa and shows expression in various cell types including those in the heart liver and lungs. As a component of focal adhesions zyxin plays a role in cytoskeletal dynamics by interacting with actin filaments and facilitating the formation of stress fibers.
- Biological function summary
The protein has important roles in cellular processes such as migration and morphology. Zyxin often forms part of a larger protein complex that includes VASP and α-actinin which contribute to cell scaffolding and structural integrity. It serves as an organizer for cytoskeletal proteins ensuring proper cell movement and adaptability to cellular stress. Its actin-binding capacity enables it to regulate structural changes necessary for cellular adaptation to environmental stimuli.
- Pathways
This protein is essential in the regulation of actin cytoskeleton associated pathways and adherens junction signaling. Zyxin interacts with Ena/VASP proteins which are significant in actin filament elongation helping regulate dynamic changes in cell shape and motility. Its relationship with LIM domain-containing proteins positions zyxin within pathways related to cell proliferation and signal transduction.
- Associated diseases and disorders
Zyxin has been implicated in cancer progression especially in metastasis where altered cell adhesion and movement play a role. It also relates to cardiovascular diseases due to its involvement in mechanotransduction pathways critical to heart muscle function. In cancer zyxin influences the activity of YAP/TAZ transcription regulators affecting tumor cell behavior. In cardiovascular scenarios the protein interacts with paxillin to mediate responses to mechanical load changes impacting heart disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![Western blot - Anti-Zyxin antibody [ZOL301] (ab50391), expandable thumbnail](https://content.abcam.com/products/images/zyxin-antibody-zol301-ab50391--western-blot-img166849.jpg "Western blot - Anti-Zyxin antibody [ZOL301] (ab50391)")
Western blot - Anti-Zyxin antibody [ZOL301] (ab50391)
Lanes 1 - 4: Merged signal (red and green). Green - ab50391 observed at 75 kDa. Red - loading control Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.ab50391 was shown to react with ZYX in wild-type HEK-293T cells in Western blot with loss of signal observed in ZYX knockout cell line Human ZYX (Zyxin) knockout HEK-293T cell line ab266504 (ZYX knockout cell lysate Human ZYX (Zyxin) knockout HEK-293T cell lysate ab257810). Wild-type HEK-293T and ZYX knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab50391 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-Zyxin antibody [ZOL301] (ab50391) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: ZYX knockout HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human ZYX (Zyxin) knockout HEK-293T cell line (Human ZYX (Zyxin) knockout HEK-293T cell line ab266504)
Lane 3: HeLa cell lysate at 20 µg
Lane 4: Daudi cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 75 kDa
![Western blot - Anti-Zyxin antibody [ZOL301] (ab50391), expandable thumbnail](https://content.abcam.com/products/images/zyxin-antibody-zol301-ab50391--western-blot-img167813.jpg "Western blot - Anti-Zyxin antibody [ZOL301] (ab50391)")
Western blot - Anti-Zyxin antibody [ZOL301] (ab50391)
False colour image of Western blot: Anti-Zyxin antibody [ZOL301] staining at 1/1000 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab50391 was shown to bind specifically to Zyxin. A band was observed at 75 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in ZYX CRISPR-Cas9 edited cell line Human ZYX (Zyxin) knockout HEK-293T cell line ab266503 (CRISPR-Cas9 edited cell lysate Human ZYX (Zyxin) knockout HEK-293T cell lysate ab257809). The band observed in the CRISPR-Cas9 edited lysate lane below 75 kDa is likely to represent a truncated form of Zyxin. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and ZYX CRISPR-Cas9 edited HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution.
All lanes: Western blot - Anti-Zyxin antibody [ZOL301] (ab50391) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: ZYX CRISPR-Cas9 edited HEK-293T cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: Daudi cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 75 kDa
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