AB78910

Anti-Zyxin (phospho S142) antibody

Name: Anti-Zyxin (phospho S142) antibody (ab78910) | Abcam
Brand: Abcam Limited
SKU: AB78910
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Zyxin phospho S142 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Zyxin phospho S142.

View Alternative Names

Zyxin, Zyxin-2, ZYX

1 Images

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Western blot - Anti-Zyxin (phospho S142) antibody (AB78910)

All lanes:

Western blot - Anti-Zyxin (phospho S142) antibody (ab78910) at 1/500 dilution

Lane 1:

Extracts from Jurkat cells, treated with paclitaxel (1uM, 24hours) at 5 µg

Lane 2:

Extracts from Jurkat cells, treated with paclitaxel (1uM, 24hours) at 5 µg with immunising phosphopeptide

Predicted band size: 61 kDa

Observed band size: 61 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human Zyxin phospho S142. The exact immunogen used to generate this antibody is proprietary information.

Q15942

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab78910 was purified by affinity chromatography using an epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Zyxin also known as ZYX is a mechanical regulator associated with cell adhesion and signaling. This protein has a molecular mass of approximately 61 kDa and shows expression in various cell types including those in the heart liver and lungs. As a component of focal adhesions zyxin plays a role in cytoskeletal dynamics by interacting with actin filaments and facilitating the formation of stress fibers.

Biological function summary

The protein has important roles in cellular processes such as migration and morphology. Zyxin often forms part of a larger protein complex that includes VASP and α-actinin which contribute to cell scaffolding and structural integrity. It serves as an organizer for cytoskeletal proteins ensuring proper cell movement and adaptability to cellular stress. Its actin-binding capacity enables it to regulate structural changes necessary for cellular adaptation to environmental stimuli.

Pathways

This protein is essential in the regulation of actin cytoskeleton associated pathways and adherens junction signaling. Zyxin interacts with Ena/VASP proteins which are significant in actin filament elongation helping regulate dynamic changes in cell shape and motility. Its relationship with LIM domain-containing proteins positions zyxin within pathways related to cell proliferation and signal transduction.

Zyxin has been implicated in cancer progression especially in metastasis where altered cell adhesion and movement play a role. It also relates to cardiovascular diseases due to its involvement in mechanotransduction pathways critical to heart muscle function. In cancer zyxin influences the activity of YAP/TAZ transcription regulators affecting tumor cell behavior. In cardiovascular scenarios the protein interacts with paxillin to mediate responses to mechanical load changes impacting heart disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity).

See full target information Zyxin phospho S142

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biochimica et biophysica acta 1853:388-95 PubMed25450971

2014

Imatinib restores VASP activity and its interaction with Zyxin in BCR-ABL leukemic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa A Bernusso,João A Machado-Neto,Fernando V Pericole,Karla P Vieira,Adriana S S Duarte,Fabiola Traina,Marc D Hansen,Sara T Olalla Saad,Karin S A Barcellos

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