AB118800

Anti-ZZZ3 antibody

Name: Anti-ZZZ3 antibody (ab118800) | Abcam
Brand: Abcam Limited
SKU: AB118800

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(2 Publications)

Rabbit Polyclonal ZZZ3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ZZZ3 aa 50-100.

View Alternative Names

ZZ-type zinc finger-containing protein 3, ZZZ3

2 Images

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Immunoprecipitation - Anti-ZZZ3 antibody (AB118800)

Detection of ZZZ3 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118800 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-ZZZ3 antibody (ab118800)

Predicted band size: 102 kDa

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Unknown

Western blot - Anti-ZZZ3 antibody (AB118800)

All lanes:

Western blot - Anti-ZZZ3 antibody (ab118800) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

HeLa whole cell lysate at 50 µg

Predicted band size: 102 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human ZZZ3 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q8IYH5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab118800 was affinity purified using an epitope specific to ZZZ3 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZZZ3 also known as Zinc Finger ZZ-Type Containing 3 serves as a component of several essential cellular processes. This protein has a molecular mass of approximately 90 kDa. Researchers have identified its expression predominantly in the nucleus across diverse human tissues indicating its widespread functional impact. Mechanically ZZZ3 acts as a transcriptional regulator important for mediating gene expression by interacting with specific DNA sequences.

Biological function summary

ZZZ3 plays a role as part of the ADA2a-containing (ATAC) histone acetyltransferase complex. This complex impacts chromatin remodeling by modifying histone acetylation influencing the accessibility of transcription factors to DNA. The protein's ability to alter chromatin structure makes it significant in regulating gene expression during cell cycle progression and differentiation.

Pathways

ZZZ3 functions prominently within chromatin organization and transcription regulation pathways. It interacts closely with proteins such as GCN5 a histone acetyltransferase to modulate these pathways. Additionally the protein is involved in the transcriptional co-activator role co-operating with various transcription factors to facilitate RNA polymerase II-driven gene transcription.

Altered ZZZ3 function links to conditions like cancer and neurological disorders. It associates with the dysregulation of gene expression observed in these diseases. In cancer ZZZ3 works in conjunction with oncogenes and tumor suppressor genes contributing to tumorigenic processes. Similarly aberrant interaction with proteins involved in neural signaling pathways can implicate ZZZ3 in certain neurological pathologies where it may affect neural development or function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone H3 reader that is required for the ATAC complex-mediated maintenance of histone acetylation and gene activation (PubMed : 30217978). Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4 (PubMed : 19103755).

See full target information ZZZ3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 222: PubMed36920342

2023

The exocyst complex is an essential component of the mammalian constitutive secretory pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Conceição Pereira,Danièle Stalder,Georgina S F Anderson,Amber S Shun-Shion,Jack Houghton,Robin Antrobus,Michael A Chapman,Daniel J Fazakerley,David C Gershlick

eLife 10: PubMed33913810

2021

TAZ-CAMTA1 and YAP-TFE3 alter the TAZ/YAP transcriptome by recruiting the ATAC histone acetyltransferase complex.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole Merritt,Keith Garcia,Dushyandi Rajendran,Zhen-Yuan Lin,Xiaomeng Zhang,Katrina A Mitchell,Nicholas Borcherding,Colleen Fullenkamp,Michael S Chimenti,Anne-Claude Gingras,Kieran F Harvey,Munir R Tanas

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZZZ3 antibody

Immunoprecipitation - Anti-ZZZ3 antibody (AB118800)

Western blot - Anti-ZZZ3 antibody (AB118800)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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Product promise