Antibody purification and concentration kits
Abcam's antibody purification and concentration kits offer you a streamlined and efficient solution for isolating and concentrating antibodies from various sources. These kits are designed with simplicity and effectiveness in mind, allowing you to achieve high-purity antibodies with minimal hands-on time. Using our advanced purification technologies, we ensure that your antibodies retain their functionality and specificity, making them ideal for a wide range of applications, including western blotting, immunohistochemistry, and ELISA.
Our purification kits come with all the necessary reagents and detailed protocols. Our antibody purification kits are designed to remove unwanted buffer constituents by capturing the antibody on agarose beads coupled to proteins A or G, leaving the antibody in a format suitable for you to transfer to a conjugation-friendly buffer. Abcam's commitment to quality is evident in the robust performance and reproducibility of our kits, which have been rigorously tested to ensure consistent results. By providing reliable and easy-to-use solutions, empower your research and bring confidence in your results with the knowledge that you are using high-quality antibodies.
Antibody purification kits
Abcam's antibody concentration and clean-up kits are designed to streamline the process of concentrating antibodies and proteins, ensuring their compatibility with various conjugation kits. Using a convenient spin column method, these kits allow you to quickly and efficiently concentrate your samples while removing small additives that could interfere with downstream applications. Their simplicity and effectiveness make them invaluable tools in your laboratory.
These kits can also be used to remove any undesirable low molecular weight additives such as Tris, glycine, and sodium azide from the antibody storage buffer that passes through a filter, trapping the antibody, which is then recovered in a conjugation-friendly buffer. Removing sodium azide is particularly important if conjugating your antibody to Horseradish Peroxidase (HRP), as sodium azide rapidly reduces HRP enzymatic activity.
It is essential to note that any buffer components with a molecular weight greater than 10 kDa, such as BSA, will also be concentrated along with the antibodies. Our comprehensive range of clean-up kits ensures that you have access to the right tools for preparing high-quality, purified antibodies and proteins for your specific needs.
Product highlights
Antibody Purification Kit (Protein A) (ab102784)
- Suitable for purifying IgG from multiple species
- Removes contaminants including BSA, glycine, tris, and azide
- Purifies antibodies from crude samples such as ascites fluid or immune serum
- Compatible with Lightning-Link® and Oligonucleotide conjugation kits
Mouse Antibody Purification Kit (ab128745)
- Suitable for purifying mouse IgG only
- Removes contaminants including BSA, glycine, tris, and azide
- Purifies antibodies from crude samples such as ascites fluid or immune serum
- Compatible with Lightning-Link® and Oligonucleotide conjugation kits
Antibody Purification Kit – Nanoparticles (ab204909)
- Suitable for purifying IgG from multiple species
- Removes contaminants including BSA, glycine, tris, and azide
- Purifies antibodies from crude samples such as ascites fluid or immune serum
- Compatible with Nanoparticle conjugation kits
Removes contaminants including BSA, glycine, tris, and azide
Purifies antibodies from crude samples such as ascites fluid or immune serum
- Lightning-Link® conjugation kits
- Oligonucleotide conjugation kits
Removes BSA, glycine, tris, and azide
- Lightning-Link® conjugation kits
- Oligonucleotide conjugation kits
Removes contaminants including BSA, glycine, tris, and azide
Purifies antibodies from crude samples such as ascites fluid or immune serum
- Nanoparticle conjugation kits
Removes contaminants including BSA, glycine, tris, and azide
Purifies antibodies from crude samples such as ascites fluid or immune serum
- Nanoparticle conjugation kits
- Lightning-Link® conjugation kits
- Oligonucleotide conjugation kits
- Lightning-Link® conjugation kits
- Oligonucleotide conjugation kits
- Lightning-Link® conjugation kits
- Oligonucleotide conjugation kits
- Nanoparticle conjugation kits
Suitable for purifying IgG fractions from serum and ascites fluid
Not suitable for purifying goat IgG*
- Lightning-Link® conjugation kits
- Oligonucleotide conjugation kits
- Lightning-Link® conjugation kits
- Oligonucleotide conjugation kits
- Nanoparticle conjugation kits
Antibody concentration
Suitable for purifying very low amounts of antibody
- Compatible with all conjugation kits
Antibody concentration kits
Abcam's antibody purification kits are designed to remove unwanted buffer constituents by capturing antibodies on agarose beads coupled to proteins A or G. This leaves the antibodies in a format suitable for transfer to a conjugation-friendly buffer. However, this method is not suitable for samples with dilute antibodies in large volumes, such as tissue culture supernatant (TCS).
For TCS purification, we offer specialized kits that effectively remove bovine IgG molecules from FBS used in cell growth media. These molecules must be removed before conjugation reactions to prevent them from competing with the target antibody for labeling. By providing tailored solutions for different purification needs, we ensure that you can achieve high-purity antibodies that are ready for a wide range of applications.
Product highlights
Antibody Concentration and Clean-Up Kit (ab102778)
Suitable for the concentration of antibodies and proteins
Remove small additives such as azide, glycine, and Tris
Compatible with Lightning-Link® and Oligonucleotide conjugation kits
Antibody Concentration and Clean-Up Kit – Nanoparticles (ab204911)
- Suitable for the concentration of antibodies and proteins
- Remove small additives such as salt, citrate, and azide
- Compatible with Gold and Magnetic Conjugation Kits
Antibody Concentration and Clean-Up Kit for Latex & Europium (ab269965)
- Suitable for the concentration of antibodies and proteins
- Remove small additives such as salt, citrate, and azide
- Compatible with Latex and Europium conjugation kits