IHC antibody solutions
Immunohistochemistry (IHC) is an immunostaining technique which selectively identifies antigens in tissues. High-quality, specific antibodies are essential to run a successful IHC experiment.
IHC is widely used in research and diagnostics to study the pathophysiology of both normal biological processes and disease states. IHC is routinely used in histopathology to detect the presence and localization of specific protein markers within a patient tissue sample. This is critical to enable accurate diagnosis, tumor classification, prognosis and treatment plans.
Abcam’s catalog offers an extensive portfolio of IHC antibodies validated to the highest industry standards to allow your research to move faster.
Recombinant antibodies
IHC has become an essential technique in clinical diagnostics. Robust, reliable, and reproducible antibodies are essential for running good IHC experiments. Our portfolio of recombinant rabbit antibodies offers over 8,700 antibodies, including the broadest range of IHC antibodies in the immuno-oncology field.
Our antibodies are validated in IHC with industry-leading processes so you can trust your reagents and your results. Antibodies are tested—during clone screening as well as final QC stages—on tissue microarrays (TMAs) comprising over 15 human normal tissue types and 15 human cancer types, all in duplicates. For markers of clinical relevance, disease-specific TMAs are also used as part of the validation process (eg breast cancer TMAs including different cancer subtypes like carcinoma, adenocarcinoma, invasive ductal carcinoma etc). Mouse and rat tissues are also included to assess species cross-reactivity where needed.
Product highlights
Anti-PD-L1 antibody [28-8]
- Among the most-cited PD-L1 clones
- Validated in relevant disease models and cell lines expressing known protein levels
- Validated in IHC-P, IHC-Fr, mIHC, ICC, FC and WB
- Available in carrier-free and conjugated formats
Anti-xCT antibody [EPR27115-64]
- Validated on the Leica BOND™ RX automated IHC staining platform
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray validation
- Usable for all your xCT staining; compatible with western blotting, IHC, immunofluorescence and flow cytometry
Anti-TRPV1 antibody [EPR25057-67]
- Rabbit monoclonal antibody used in TRPV1 western blotting and IHC
- Suitable for human, mouse and rat samples
- Validated on the Leica BOND™ RX automated IHC staining platform
Multiplex-ready antibodies
IHC is a powerful widely used diagnostic tool in histopathology, but analysis is limited to only one marker per tissue section. Multiplex IHC (mIHC) combines the power of IHC with the ability to image several markers within the same tissue section.
The extensive validation of our portfolio of recombinant IHC antibodies ensures that our products are compatible with many mIHC platforms (eg Akoya, Nanostring, 10X Genomics).
We also offer a large portfolio of IHC-validated antibodies conjugated with fluorescent dyes that allow staining of multiple markers on the same tissue section without the need for a secondary antibody, streamlining the protocol and saving you time.
Product highlights
Anti-CD11b antibody [EPR1344]
- Part of a panel of three antibodies for studying monocytes and macrophages
- Compatible with Akoya Tyramide technology
Anti-NeuN antibody [EPR12763]
- Part of a panel for profiling neurons and astrocytes
- Compatible with Akoya Tyramide technology
- Also validated for chromogenic mIHC on the Roche Ventana Discovery Ultra
Anti-Progesterone Receptor antibody
- Part of a panel of 3 antibodies for hormonal profiling of breast cancer tissues
- Compatible with Akoya Tyramide technology
Antibody panels
Abcam's antibody panels offer reliable, high-quality tools to support various research applications. Designed to streamline the study of biological markers and pathways, our panels are ideal for investigating cellular processes, disease mechanisms, and therapeutic targets. Whether focused on exosomes, stem cells, immune cells, or signaling pathways, these products give you ready-to-use solutions to achieve accurate and reproducible results.
Validated for performance and covering a broad spectrum of targets, Abcam's panels ensure confidence in experimental outcomes. With options suitable for human and mouse models, they cater to diverse research needs. By combining quality, ease of use, and comprehensive coverage, these panels are a valuable tool in your arsenal.
Product highlights
Exosome panel
- Includes antibodies to calnexin, CD9, CD63, CD81, Hsp70, TSG101
- Suitable for studying exosome biogenesis, tracking and release, cancer and neurodegenerative research, and extracellular vesicles research
Embryonic stem cell marker panel
- Includes antibodies to Oct4, Nanog, Tra-1-60 (R), SOX2, SSEA4
- Suitable for characterizing and validating human embryonic stem cells, developmental biology, and regenerative medicine
PI3K/AKT signaling pathway panel
- Includes antibodies to various PI3K/AKT pathway components
- Suitable for research on cell proliferation, survival and metabolism, as well as cancer and signal transduction studies
Immunostaining, detection systems and counterstains
View our range of secondary antibodies, conjugation kits and detection kits suitable for use in imaging studies.
Product highlights
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)
- Rated 5* by customers
- Suitable for IHC-Fr, IHC-P, ICC/IF and Flow cytometry
Goat Anti-Rabbit IgG H&L (HRP)
- Flexible signal amplification- use in colorimetric or chemiluminescent detection
- Proven performance: cited in over 3565 publications
Rabbit Anti-Mouse IgG H&L (HRP)
- Ideal for western blot
- Suitable for ICC, IHC, western blot, dot blot and ELISA.
- Cited in over 1090 publications
Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 200 publications
Buffers, mounting media and other accessories
Easily find the accessory reagents you need, from sample prep and wash buffers to mounting media.
Product highlights
PAP pen
The PAP pen is a special marking pen that provides a thin film-like green-tinged hydrophobic barrier when a circle is drawn gently around a specimen on a slide.
Mounting Medium With DAPI - Aqueous, Fluoroshield
Aqueous anti-fade mounting medium designed to preserve fluorescence when imaging tissues and cell samples.
Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0)
Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) enables target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step.
