Immunocytochemistry reagents

IgorE Abcam ICC

Immunocytochemistry (ICC), commonly referred to as immunofluorescence (IF), is a valuable tool for detecting and visualizing proteins— or other antigens— within cultured or primary cells. ICC/IF is also used to measure and compare cellular phenotypes and the impact of pathway alterations in diseased and engineered cells.

Abcam’s broad catalog of quality reagents includes conjugated primary and secondary antibodies, as well as chimeric antibodies that offer you greater flexibility in host species when running low-plex ICC/IF experiments. Over 2,000 of our antibodies are validated using knock-out cell lines to ensure specificity.

Explore our range and find out how we can help you detect a broad range of cellular targets, including structural markers, organelles, cell fate markers, and key targets that drive complex disease phenotypes.

ICC-validated recombinant antibodies

Our catalog of over 10,000 ICC/IF-validated recombinant antibodies covers a broad range of phenotypes, including metabolism, apoptosis, stress responses, proteostasis, autophagy, and cell-to-cell adhesion, as well as different organelle and structural markers. Many of our antibodies are available in conjugated formats to give you greater multiplex flexibility and are knock-out validated to ensure ultimate specificity.

Recombinant antibodies are invaluable tools in your study of complex diseases, including cancer and neurodegenerative diseases. For example, our ICC/IF-validated recombinant antibodies are commonly used to monitor tumor initiation and progression, as well as interactions with the immune system. Explore a sample of our most highly-cited ICC/IF-validated antibodies below.

Product highlights

Target
Product name
abID
Cellular feature/ phenotype
Listed applications
Conjugates available
Ki67
Anti-Ki67 antibody [SP6]
ab16667
Proliferation
Flow Cyt (Intra), ICC/IF, IHC-P, WB, mIHC
4
Vimentin
Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker
ab92547
Cytoskeletal
Flow Cyt (Intra), ICC/IF, IHC-P, WB, mIHC
9
NeuN
Anti-NeuN antibody [EPR12763] - Neuronal Marker
ab177487
Neuronal
Flow Cyt (Intra), ICC/IF, IHC (PFA fixed), IHC-Fr, IHC-P, WB, mIHC
7
LC3B
Anti-LC3B antibody [EPR18709] - Autophagosome Marker
ab192890
Autophagosome
ICC/IF, IHC-P, WB
3
Paxillin
Anti-Paxillin antibody [Y113]
ab32084
Cell adhesion
Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB
2
ACTG2
Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184]
ab32575
Cytoskeletal
ELISA, Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, WB
5
gamma H2A.X
Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]
ab81299
DNA damage
Dot, ICC/IF, IHC-P, IP, WB
4
E Cadherin
Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker
ab40772
Junctional
Flow Cyt (Intra), ICC/IF, IHC-P, WB, mIHC
6
Rad51
Anti-Rad51 antibody [EPR4030(3)]
ab133534
DNA damage
Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB
6
CD68
Anti-CD68 antibody [KP1]
ab955
Macrophage
ICC/IF, IHC-P, Mass Cytometry, WB
4
GM130
Anti-GM130 antibody [EP892Y] - cis-Golgi Marker
ab52649
Golgi
Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB
5
S100 beta
Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker
ab52642
Astrocyte
ICC/IF, IHC-Fr, IHC-P, WB
5
TOMM20
Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker
ab186735
Mitochondrial
Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, WB
7
Iba1
Anti-Iba1 antibody [EPR16588]
ab178846
Microglia
Flow Cyt (Intra), ICC/IF, IHC-P, WB
2
c-Myc
Anti-c-Myc antibody [Y69] - ChIP Grade
ab32072
Proliferation
ChIC/CUT&RUN-seq, ChIP-seq, Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB
5
Olig2
Anti-Olig2 antibody [EPR2673]
ab109186
Oligodendrocyte
ICC/IF, IHC-P, Mass Cytometry, WB
5
Glutathione Peroxidase 4
Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]
ab125066
Stress response
Flow Cyt (Intra), ICC/IF, IHC-P, WB
2
Sodium Potassium ATPase
Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control
ab76020
Plasma membrane
Flow Cyt (Intra), ICC/IF, IHC-P, WB
6
BRG1
Anti-BRG1 antibody [EPNCIR111A]
ab110641
Chromatin remodeling
ChIC/CUT&RUN-seq, Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB
4
GFP
Anti-GFP antibody [EPR14104]
ab183734
Fluorescent tag
Flow Cyt (Intra), ICC/IF, IHC-P, WB
3

Explore our full range of precision recombinant antibodies validated in ICC

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Chimeric antibodies

Multiplexing ICC/IF experiments can be challenging, particularly when using secondary antibodies that require each primary antibody within the panel to be raised in a different host species. As a result, your research may rely on polyclonals raised in different species, making you sacrifice specificity and reproducibility. Chimeric antibodies solve this problem, giving you greater host species flexibility while retaining great antibody quality.

To generate our chimeric antibodies, we engineer the antigen-binding region of our precision recombinant monoclonal antibodies (generated in rabbits as the host species) onto the constant region of an alternative species (eg mouse, rat or chicken). The outcome is an antibody that offers you the same premium performance, including high specificity and reproducibility, but which can be used with an alternative secondary antibody.

Our growing range of over 250 chimeras includes common targets used in ICC/IF experiments.

Product highlights

Browse our full range of chimeric antibodies

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Secondary antibodies

Secondary antibodies conjugated to fluorophores, like Alexa Fluor® dyes, are vital reagents for improving sensitivity and detecting low-abundance targets. Our catalog includes secondary antibodies conjugated to nine Alexa Fluor® dyes, which are ideal for multi-color analysis and use in ICC/IF experiments. Learn about Alexa Fluor® conjugated secondary antibodies at Abcam here and explore our most popular products below.

Product highlights

Explore our full range of secondary antibodies

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Cellular dyes

In addition to the antibodies you need for immunostaining, we offer a range of fluorescent dyes that stain specific cellular compartments, ranging from mitochondria to the endoplasmic reticulum. These can provide valuable information on cell structure and localization while providing further flexibility when trying to observe multiple targets or compartments.

For example, our Cytopainter dyes can be used in live cells and will be retained within compartments after fixation, meaning they can also be used in co-staining experiments that use antibodies. Our dyes are easy to integrate into your existing staining protocols and have high photostability to provide longer exposure times.

Product highlights

View all our accessory reagents and tools for sample prep and detection

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Knock-out and iPSC cell lines

Our cell lines provide vital insights into complex diseases. Our catalog contains genetically engineered knock-out cell types relevant to neuroscience and oncology targets (including Raji, THP-1, MCF7, and SH-SY5Y), as well as induced pluripotent stem cell (iPSCs) lines specifically designed to model diseases. Available iPSC-derived disease models include Huntington’s disease, Amyotrophic lateral sclerosis (ALS), and frontotemporal dementia (FTD).

Explore some of our most popular cell lines and disease models below.

Product highlights

Explore our full range of over 4,500 cell lines

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