Immunocytochemistry reagents

Immunocytochemistry (ICC), commonly referred to as immunofluorescence (IF), is a valuable tool for detecting and visualizing proteins— or other antigens— within cultured or primary cells. ICC/IF is also used to measure and compare cellular phenotypes and the impact of pathway alterations in diseased and engineered cells.
Abcam’s broad catalog of quality reagents includes conjugated primary and secondary antibodies, as well as chimeric antibodies that offer you greater flexibility in host species when running low-plex ICC/IF experiments. Over 2,000 of our antibodies are validated using knock-out cell lines to ensure specificity.
Explore our range and find out how we can help you detect a broad range of cellular targets, including structural markers, organelles, cell fate markers, and key targets that drive complex disease phenotypes.
ICC-validated recombinant antibodies
Our catalog of over 10,000 ICC/IF-validated recombinant antibodies covers a broad range of phenotypes, including metabolism, apoptosis, stress responses, proteostasis, autophagy, and cell-to-cell adhesion, as well as different organelle and structural markers. Many of our antibodies are available in conjugated formats to give you greater multiplex flexibility and are knock-out validated to ensure ultimate specificity.
Recombinant antibodies are invaluable tools in your study of complex diseases, including cancer and neurodegenerative diseases. For example, our ICC/IF-validated recombinant antibodies are commonly used to monitor tumor initiation and progression, as well as interactions with the immune system. Explore a sample of our most highly-cited ICC/IF-validated antibodies below.
Product highlights
Anti-Ki67 antibody [SP6]
- Cited in over 2500 publications
- Knock-out validated
- Five-star rating with over 120 customer reviews

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker
- Cited in over 1700 publications
- Advanced validation
- Five-star rating with over 20 customer reviews

Anti-NeuN antibody [EPR12763] - Neuronal Marker
- Cited in over 850 publications
- Broadly validated across applications and reactive species
- Five-star rating with over 70 customer reviews

Chimeric antibodies
Multiplexing ICC/IF experiments can be challenging, particularly when using secondary antibodies that require each primary antibody within the panel to be raised in a different host species. As a result, your research may rely on polyclonals raised in different species, making you sacrifice specificity and reproducibility. Chimeric antibodies solve this problem, giving you greater host species flexibility while retaining great antibody quality.
To generate our chimeric antibodies, we engineer the antigen-binding region of our precision recombinant monoclonal antibodies (generated in rabbits as the host species) onto the constant region of an alternative species (eg mouse, rat or chicken). The outcome is an antibody that offers you the same premium performance, including high specificity and reproducibility, but which can be used with an alternative secondary antibody.
Our growing range of over 250 chimeras includes common targets used in ICC/IF experiments.
Product highlights
Anti-Ki67 [SP6] – Chicken IgY (Chimeric)
- Same target specificity as parent clone, different host species
- Ideal for multiplexing using secondary antibodies

Anti-Vimentin antibody [EPR3776] - Chicken IgY (Chimeric)
- Same target specificity as parent clone, different host species
- Ideal for multiplexing using secondary antibodies

Anti-NeuN antibody [EPR12763] - Rat IgG2a (Chimeric)
- Same target specificity as parent clone, different host species
- Ideal for multiplexing using secondary antibodies

Secondary antibodies
Secondary antibodies conjugated to fluorophores, like Alexa Fluor® dyes, are vital reagents for improving sensitivity and detecting low-abundance targets. Our catalog includes secondary antibodies conjugated to nine Alexa Fluor® dyes, which are ideal for multi-color analysis and use in ICC/IF experiments. Learn about Alexa Fluor® conjugated secondary antibodies at Abcam here and explore our most popular products below.
Product highlights
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)
- Featured in over 3,000 citations
- Suitable for ICC/IF, IHC-Fr, IHC-P and flow cytometry

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)
- Featured in over 600 citations
- Suitable for ICC/IF, IHC-Fr, IHC-P, flow cytometry and ELISA

Cellular dyes
In addition to the antibodies you need for immunostaining, we offer a range of fluorescent dyes that stain specific cellular compartments, ranging from mitochondria to the endoplasmic reticulum. These can provide valuable information on cell structure and localization while providing further flexibility when trying to observe multiple targets or compartments.
For example, our Cytopainter dyes can be used in live cells and will be retained within compartments after fixation, meaning they can also be used in co-staining experiments that use antibodies. Our dyes are easy to integrate into your existing staining protocols and have high photostability to provide longer exposure times.
Product highlights
Cell tracking dye kit
- Uniform cell labeling for multicolor analysis
- Robust labeling process requiring minimal hands-on time
- Can be used with multiple fluorescent readouts, including flow cytometry and microscopy

Cell Plasma Membrane Staining kit
- Uniform staining across a wide variety of mammalian cell types
- Staining remains after fixation with formaldehyde, enabling further multicolor staining

DRAQ5™
- Cell-permeable far-red fluorescence DNA dye
- Usable in fixed or live cells in combination with common labels like GFP and FITC

DRAQ7™
- Selectively stains the nuclei of dead, permeabilized cells; can be used in place of propidium iodide
- Usable in combination with common labels like GFP or FITC
- Rapid staining; no wash needed

Knock-out and iPSC cell lines
Our cell lines provide vital insights into complex diseases. Our catalog contains genetically engineered knock-out cell types relevant to neuroscience and oncology targets (including Raji, THP-1, MCF7, and SH-SY5Y), as well as induced pluripotent stem cell (iPSCs) lines specifically designed to model diseases. Available iPSC-derived disease models include Huntington’s disease, Amyotrophic lateral sclerosis (ALS), and frontotemporal dementia (FTD).
Explore some of our most popular cell lines and disease models below.
Product highlights
Human TREM2 knockout THP-1 cell line
- Knock-out validated by next-generation sequencing and western blot
- Free wild-type control available

ioSkeletal Myocytes - Human iPSC derived cells
- Human iPSC-derived skeletal myocytes deterministically programmed using opti-ox, a precise cellular reprogramming technology from bit.bio
- Demonstrates robust expression of key myofilament proteins (Desmin, Titin, Troponin, Myosin Heavy Chain and Dystrophin)
- Reliable model for studying muscle and associated neuromuscular and metabolic disorders

Microglia - Human iPSC derived cells (Male, WC-30)
- Confirmed expression of microglial surface markers, including CD45, CD11b, CX3CR1, P2RY12 and TMEM119
- A GFP-expressing alternate is also available
