Low-plex multiplex IHC for biomarker analysis
Explore our highly validated low-plex multiplex IHC antibodies for biomarker analysis. Achieve accurate detection of multiple markers in a single tissue section.
Multiplex immunohistochemistry (mIHC) is a technique that enables the simultaneous detection of multiple biomarkers within a single tissue section. It offers deeper insights into cellular interactions and spatial context.
Our antibody panels are carefully designed to study essential biological pathways, allowing researchers to observe cellular signaling dynamics accurately.
While a practical approach, selecting antibodies for mIHC can be complex and time-consuming, especially when targeting low-expression markers. To support translational research and biomarker validation, we’ve rigorously tested and validated low-plex panels (over 500 antibodies), ensuring that selected clones not only perform reliably on multiplex platforms but also work cohesively together in biologically relevant combinations. This helps reduce trial-and-error and simplify your experimental design.
Our catalog features a diverse range of antibodies validated for mIHC, tested using Leica Biosystems BOND® MAX and Opal™ reagents from Akoya Biosciences, two widely adopted platforms in high-throughput tissue analysis. This robust validation ensures compatibility with automated staining workflows and signal amplification technologies, allowing researchers to use their own standard microscopes and simplifying workflow integration.
Browse our selection to find antibodies that have been thoroughly tested for performance in multiplex IHC. With validation on trusted platforms and compatibility with advanced imaging systems, you can move forward with greater confidence in your results.
Immune cell markers
Understanding the immune landscape within tissues is imperative for advancing immuno-oncology, infectious disease, and inflammation research.
mIHC can be used to detect multiple immune cell markers, such as CD4, CD11b, CD68, CD19, and F4/80, within a single tissue section, maintaining spatial context and cellular interactions.
Browse our portfolio to find the right immune cell markers for your mIHC workflow.
Anti-FOXP3 antibody [236A/E7]
- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency
- Over 590 publications
- Trusted since 2005
Anti-CD163 antibody [EPR19518]
- Clone EPR19518 is the most cited clone to CD163
- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency
- Over 390 publications
Anti-CD4 antibody [EPR6855]
- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency
- Over 310 publications
Anti-CD3 epsilon antibody [SP7]
- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency
- Over 720 publications
- Trusted since 2005
Morphology markers
Morphology markers provide spatial insight into tissue architecture by labeling anatomical features, cell types, and compartments. These markers highlight relationships between biological structures, helping to identify regions of interest (ROI) and interpret how cells are organized within their microenvironments. Common targets include blood vessels (eg, CD31), lymphatic vessels (eg, CD31), lymphatic vessels (eg, podoplanin), nerve fibers (eg, neurofilament), fibroblasts (eg, FAP), and extracellular matrix components (eg, collagen, laminin).
These markers visualize these elements, support the distinction between tissue compartments, and reveal how immune, tumor, and stromal cells interact. In mIHC workflows, they are often used alongside immune or tumor-specific markers to study tissue remodeling and cellular dynamics. This approach is widely applied in research areas such as tumor microenvironment analysis, wound healing, and developmental biology.
Because mIHC preserves spatial relationships, morphology markers help anchor biological observations within the physical context of tissue structure. Their use enhances high-content tissue analysis by providing clarity and context across diverse biological samples.
Anti-CD31 antibody [EPR17259]
- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency
- Over 220 publications
Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker
- KO validated for confirmed specificity
- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency
- Over 1730 publications
Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker
- KO validated for confirmed specificity
- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency
- Over 750 publications
Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker
- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency
- Over 230 publications
- Trusted since 2007
