Drug discovery solutions for obesity research
As obesity rates have risen, our understanding has evolved to recognize the condition as a complex, multifactorial disease characterized by energy balance dysregulation. The need for effective pharmacotherapeutics has never been so urgent.
We collaborate with key opinion leaders in obesity research to develop proteomic tools that accelerate your drug discovery efforts. Our reagents can take you from start to end in the drug development process, from early-stage target identification and validation to late-stage clinical trials and biomarker development.
Our broad range of gold-standard reagents features highly specific recombinant antibodies and kits, validated across applications and species to ensure the precision and reproducibility your work requires.
Drive your drug discovery efforts forward with our tailored solutions designed to meet the unique challenges of obesity research.
Tools to investigate hormone-based therapeutic targets for obesity treatment
Recognizing the role of hormones in appetite regulation and metabolic disorders has ushered in a transformative new era of obesity treatments; chiefly GLP-1 receptor agonists 1. These can be used alone, in combination with other hormone-based medications, or as multi-receptor agonists2.
Our comprehensive range of recombinant antibodies and fast, precise SimpleStep ELISA® kits can accelerate your study of obesity-related hormones, like GLP-1 and GIP, and their signaling pathways.
We know your work demands precision, so we subject our reagents to robust validation processes: our reagents are validated in relevant sample types to ensure sensitivity, and cross-reactivity data ensures specificity. We offer antibodies to assess target expression across a range of applications, including IHC, WB, ICC, IP and flow cytometry. Our extensive IHC validation features testing on the Leica® BOND™ platform to ensure compatibility and reliability.
We use our precision recombinant antibodies to give our SimpleStep ELISA® kits ultimate sensitivity and reproducibility. Their one-wash protocol reduces assay to just 90 minutes, so you can get both speed and quality from one kit.
Browse our curated range of precision antibodies and ELISA kits to find the right tools to support your research.
Product highlights
Anti-GLP-1R antibody [EPR21819] (ab218532)
- Top-cited antibody for this target
- Excellent staining in pancreas tissue
- Highlighted in a review from the Lancet group of journals3 for detecting the endogenous protein
- Highlighted by a research group at the University of Copenhagen4 showing clear membrane-localized labeling of pancreatic islets
- Suitable for WB, IHC-Fr and IHC-P; reacts with mouse and rat samples
Anti-Glucagon antibody [EP3070] (ab92517)
- Top-cited recombinant antibody to this target (>100 citations)
- Extensively validated in human, mouse and rat pancreatic tissues and cell lines
- Seven 5* reviews
- Suitable for IHC-P and WB; reacts with human, mouse and rat samples
Human GLP1 (7-36) ELISA Kit (ab184857)
- Detects active peptide (7-36)
- Measures human GLP-1 with picogram sensitivity (25 pg/mL)
- Validated in multiple biological sample types
- Single-wash 90-minute protocol
References
- Gribble, F. M. & O’Rahilly, S. Obesity therapeutics: The end of the beginning. Cell Metabolism 33, 705–706 (2021).
- Jastreboff, A. M. & Kushner, R. F. New frontiers in obesity treatment: GLP-1 and nascent nutrient-stimulated hormone-based therapeutics. Annual Review of Medicine 74, 125–139 (2023).
- DJ;, A. J. J. Reagents and models for detecting endogenous GLP1R and GIPR. EBioMedicine (2021).
- Bjørnholm, K. D. et al. Decreased expression of the GLP-1 receptor after segmental artery injury in mice. Journal of Endocrinology 248, 289–301 (2021).
Recombinant antibodies to adipogenesis and lipid metabolism markers
Understanding adipocyte differentiation and lipid storage regulation is vital for developing therapies to reduce fat accumulation and improve metabolic function. Our broad offering of recombinant antibodies meets all your research needs– both fundamental and applied research needs– in understanding and developing therapies to combat obesity. Our recombinant antibodies can accelerate your drug discovery workflow, helping to validate drug targets and screen compounds that may influence fat cell formation or lipid storage. The versatility of our antibodies makes them perfect for western blot, IP, ELISA, IHC and flow cytometry, so you can explore your function of choice (such as transcription factors, enzymes, adipocyte function and mitochondrial function). Our industry-leading validation processes guarantee specificity, and recombinant technology gives you unrivaled batch-to-batch consistency so you’ll get comparable results across studies.
Streamline your study of critical obesity pathways and biomarkers with our precision, extensively validated recombinant antibodies.
Product highlights
Anti-FABP4 antibody [EPR3579] (ab92501)
- Top-cited antibody for this target with
100 citations
- Suitable for mIHC, WB, ICC/IF and IHC-P; reacts with human, mouse and rat samples
- Optimized for the Leica® BOND™ system
- Fluorescence multiplex immunohistochemistry data available, featuring specific staining in adipocytes
Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
- Over 80 citations
- Validated for WB, IHC-P, IP, ICC/IF and flow cytometry (intra)
- Reacts with human, mouse and rat samples
- Knock-out validated
Immunoassays to adipogenesis and lipid metabolism
Our SimpleStep ELISA® kits are potent tools for screening multiple proteins of interest, generating data in just 90 minutes without compromising on performance. Powered by our recombinant antibodies, our kits are highly sensitive and specific, and provide reproducible results between assays and between labs.
ELISA kits are essential tools in obesity research, particularly when studying adipogenesis and lipid metabolism; our broad catalog ensures you’ll find a kit to accelerate your study of your target or pathway of interest.
To support high-throughput experiments, we offer a growing range of our SimpleStep ELISA® kits in a 384-well format, as well as 10 x 96-well packs. The 384-well format uses the same simple, rapid protocol and yields 300% more data while using just a quarter of the sample volume. Our 384-well kits can be an orthogonal solution to homogeneous assays by eliminating false positives, supporting your drug discovery work.
Product highlights
Human Leptin ELISA Kit (ab179884)
- Single-wash 90-minute protocol
- Measures human leptin with high sensitivity (4.65 pg/mL)
- Validated in plasma, cell culture supernatants, and serum
Mouse Adiponectin ELISA Kit (ab226900)
- Over 80 citations
- Validated for WB, IHC-P, IP, ICC/IF and flow cytometry (intra)
- Reacts with human, mouse and rat samples
- Knock-out validated
Bioassays for obesity research
Our assay kits enable comprehensive analysis and quantification of metabolic processes and dysfunctions associated with obesity, including lipid and carbohydrate metabolism, energy regulation, and oxidative stress. Effortlessly study fat accumulation, insulin resistance and obesity-related inflammation by measuring enzyme activity, metabolites and metabolic processes in cells, tissues and liquid samples.
To provide you with the best assays possible, we manufacture our kits with strict quality control criteria in our Boston, MA life sciences hub. As such, we can guarantee reliable supply so you can scale up your research with confidence, with batch sizes up to 500 kits available.
Our kits feature easy-to-use, step-by-step procedures, with controls and standards included, as well as convenient colorimetric, fluorometric and luminescent readouts.
Explore our curated range below, and discover how our kits can help you quantify free fatty acids, triglycerides, cholesterol, carbohydrate metabolism, oxidative stress and more.
Product highlights
Free Fatty Acid Assay Kit – Quantification (ab65341)
- No-wash, addition-only assay with two 30-minute incubations
- Can be used with bodily fluids, culture supernatants and cell/tissue lysates
- Colorimetric, fluorometric and luminescent readouts
Triglyceride Assay Kit – Quantification (ab65336)
- No-wash assay with two incubation steps (20 and 60 minutes)
- Can be used with urine, plasma, tissue extracts, serum, other biological fluids and cell lysates
- Colorimetric, fluorometric and luminescent readouts
