Staining lab essentials
Keep your western blot workflow moving forward.
Loading control and tag antibodies against targets such as GAPDH, actin, GFP, and tubulin are essential for ensuring the accuracy and reproducibility of your experimental results. Their role is particularly important when experiments are being published in high-impact journals, where robust data is essential.
Abcam offers an extensive range of loading control and tag antibodies, offering high performance, consistency, and extensive validation across a variety of applications. A high dilution factor minimizes the need for optimization, enabling you to perform more experiments per vial of antibody. Coupled with new lower prices, this offers exceptional value and keeps your research moving forward, faster.
With our everyday essential reagents for western blot staining, you can find everything you need to keep your workflows moving forward. All our antibodies undergo extensive validation and stringent QC methods, so you can be confident in reliable reagents and premium quality.
Tags
Discover our extensive range of loading control and tag antibodies, targeting proteins such as GAPDH, actin, GFP, and tubulin, as well as widely used fusion tags including Myc, GST, His, Strep-tag, and DDDK (Flag®). Whether you're working with endogenous proteins or tagged constructs, our high-performance antibodies offer consistency and extensive validation across a variety of applications. A high dilution factor minimizes the need for optimization, allowing you to perform more experiments per vial. Now available at lower prices, so you can be confident you're getting exceptional value.
Product highlights
Anti-mCherry antibody
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Cited in over 470 publications (CiteAb)
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Validations in multiple applications
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Rated 5* by customers
6X His tag
- Cited in over 350 publications (CiteAb)
- Rated 5* by customers
DDDDK tag / FLAG tag
- Cited in over 100 publications (CiteAb)
Loading controls
Loading controls are essential for ensuring the accuracy and reproducibility of your experimental results. Their role is particularly important when experiments are being published in high-impact journals, where robust data is essential.
Product highlights
Anti-GAPDH antibody [EPR16891] - Loading Control
- Citated in over 3,000 publications (CiteAb)
- Recombinant manufacture ensures batch-to-batch consistency
- Broadly validated across reactive species & applications
Anti-beta Actin antibody [mAbcam 8226] - Loading Control
- Highly cited with over 4,000 publications (CiteAb)
- Validated in multiple applications and species
- Rated 5* by customer
Sample prep and quantification
Good sample preparation is the first step towards a successful western blot. We offer quality reagents to help you effectively and accurately prepare your sample for western blot, ranging from lysis buffers and fractionation kits to protein quantification kits.
Product highlights
InstantBlue® Protein Stain (ISB1L)
- Rapid staining with well-defined blue bands in 15 minutes or less
- Single-step procedure eliminating the need to wash, fix, or destain
- Efficient – Only 25 ml per gel
Whole cell extraction kit
- Simple and selective kit for extracting whole cell proteins
- Works in 45 mins
- Suitable for suspension and adherent cells
Protein controls, secondaries and other accessories
Find the other accessory reagents you need for every step of your western blot workflow, including control proteins, molecular weight markers, nitrocellulose membranes and wash buffers.
Product highlights
Western blot resources
With our everyday essentials for western blot staining and a commitment to rigorous validation and quality control, we provide not only the tools but also the confidence to keep your research moving forward, faster. Check out our top western blot resources below.
The complete western blot guide
Brush up on the ins and outs of western blot, including recipes for buffers and stock solutions, recommended controls, sample preparation, and more in our complete guide.
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Troubleshooting tips for western blot
Curated by our in-house scientists, our western blot troubleshooting covers possible problems, causes, and solutions that may arise in your workflows.
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Western blot demo
In our quick 15-minute tutorial, we take you through the entire western blot workflow, from sample preparation all the way through to detection. Follow along step by step and streamline your workflow with expert guidance, powered by premium quality products.
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Western blotting at 40
Western blotting may be 40+ years old, but it’s still unlocking answers in research today. In our on-demand webinar, we walk you through the western blot process, including how to choose the right samples, select proper controls, and pick the best-suited antibodies.
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Western blot protocol
Follow our easy-to-use Western blot protocol for a step-by-step guide to mastering the technique, powered by high-quality antibodies from our Lab Essentials range, offering exceptional value without compromising performance.
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