Tools to study c-Myc and Myc-tagged proteins
c-Myc is a proto-oncogene that encodes a transcription factor crucial for cell proliferation, growth, and differentiation. Within the Myc family of transcription factors (including N-Myc and L-Myc), c-Myc regulates key genes involved in DNA replication, protein synthesis, metabolism, and cell cycle progression. It operates within a complex signaling network, modulating about 15% of human genes by binding to specific E-box sequences in DNA and forming heterodimers with Max to either promote or repress transcription. Additionally, c-Myc regulates apoptosis, balancing cell survival and death under normal conditions.
Dysregulation of c-Myc is a hallmark of various cancers, leading to uncontrolled cell proliferation and tumor progression through gene amplification, chromosomal translocation, or increased protein stability. For instance, in Burkitt's lymphoma, a translocation between chromosomes 8 and 14 places the c-Myc gene under the immunoglobulin heavy chain enhancer, causing its overexpression in B cells. In other cancers like breast, colorectal, and lung cancer, c-Myc amplification drives oncogenesis by promoting abnormal cell division and growth. Overexpression of c-Myc can also lead to metabolic reprogramming, such as the Warburg effect, where cancer cells prefer glycolysis even in the presence of oxygen to meet the energy demands of rapid proliferation.
Given its central role in cellular processes and cancer development, c-Myc is a crucial target for research and therapeutic development. Studying c-Myc expression and function in normal and diseased tissues can provide insights into tumor biology and potential treatments. Directly targeting c-Myc remains challenging due to its "undruggable" nature, but researchers are exploring indirect methods, such as inhibiting its interaction with Max or targeting downstream effectors.
Recombinant antibodies
For endogenous c-Myc, try our highly validated RabMAb® rabbit monoclonal antibodies, which are also available directly conjugated to HRP and Alexa Fluor® dyes. To study the regulation of c-Myc, our phospho-specific antibodies will help you get your experiments underway.
Our antibodies cover a wide range of applications and species, and our phospho-specific antibodies target key phosphorylation sites. We extensively validate our antibodies to ensure specificity using KO cell lines, peptide assays, and cell treatments.
Product highlights
Anti-c-Myc antibody [Y69] - ChIP Grade
- Highly cited (>1500 citations)
- Validated for several applications with human, mouse, and rat reactivity, including HC-P, IP, western blot,
- ICC/IF, ChIC/CUT&RUN-seq, ChIP-seq, and Flow Cyt (Intra).
- Available in carrier-free and conjugated formats
Anti-c-Myc (phospho-T58) antibody [EPR17923]
- RabMab specific to c-myc phosphorylated at Thr 58
- Suitable for Dot, western blot, ICC/IF, Flow Cyt (Intra) with human samples
- One of the most cited antibodies to this post-translational modification
Anti-c-Myc (phospho S62) antibody [EPR17924]
- RabMab specific to c-myc phosphorylated at Ser 62
- Human, mouse, and rat reactivity for a wide range of applications including IHC-P, ICC/IF, Flow Cyt (Intra), western blot
- One of the most cited antibodies to this post translational modification
Immunohistochemical analysis of paraffin-embedded Human endometrium cancer tissue for c-Myc (phospho-S62) using ab185656 at a 1:500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1:500 dilution. Nuclear staining of the cells is observed. Cells were counter-stained with Hematoxylin. Secondary antibody-only control: PBS was used instead of the primary antibody; the secondary antibody was a Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1:500 dilution.
Heat-mediated antigen retrieval was performed using with Tris/EDTA buffer pH 9.0 before commencing with the IHC staining protocol.
Tagged recombinant antibodies
Our fluorescently tagged anti-Myc antibodies can be used to detect proteins of interest that have been tagged with a Myc epitope. The Myc-tag, a short peptide derived from the c-Myc protein, is commonly fused to the N- or C-terminus of a target protein, allowing easy tracking in various biological assays. Abcam’s anti-Myc tagged antibodies, including our direct conjugates, are designed to allow recognition of this tag, providing a reliable tool for detection in experiments. These antibodies enable researchers to isolate or visualize tagged proteins in complex samples, whether they are working with cell lysates, tissues, or live cells. Direct conjugates streamline workflows by eliminating the need for secondary antibodies, making detection faster, and reducing background noise. This is highly useful for applications where quick and accurate results are required, such as in protein localization studies, protein-protein interaction assays, or purification steps using affinity chromatography.
Product highlights
Anti-Myc tag antibody [9E10]
- Highly cited Myc tag antibody (500 citations)
- Highly validated across several applications including Flow Cyt, ELISA, western blot, and ICC/IF
- Highly rated (29 5* Abreviews)
Alexa Fluor® 488 Anti-Myc tag antibody [9E10]
- Suitable for Flow cytometry (intracellular) and ICC/IF
Alexa Fluor® 647 Anti-Myc tag antibody [9E10]
- Suitable for Flow cytometry (intracellular) and ICC/IF
Proteins and peptides
Recombinant c-Myc proteins can be used as a powerful tool for validating reagent specificity or serving as a protein control in a wide range of assays. By integrating our high-quality recombinant c-Myc proteins into your experimental workflow, you can ensure that antibodies or other reagents targeting c-Myc recognize the protein accurately, reducing the risk of non-specific binding and enhancing experimental reliability. These recombinant proteins are particularly useful as positive controls in Western blotting, ELISA, or functional assays, where they can be used to determine the effect of c-Myc without relying solely on endogenous protein levels, which may vary across samples. Our recombinant c-Myc proteins can also be used in protein-protein interaction studies, helping researchers understand how c-Myc interacts with other cellular components in various biological contexts.
Our recombinant c-Myc products are manufactured to meet the highest standards, featuring low endotoxin levels, making them suitable for sensitive applications such as cell-based assays and in vivo studies. These proteins have been extensively validated for bioactivity, ensuring they are fully functional and capable of recapitulating the biological effects of native c-Myc in your experiments. Whether you're using them in binding studies, functional assays to assess cellular growth or differentiation, or as benchmarks to validate new reagents, our recombinant c-Myc proteins provide reliable and reproducible results.
Product highlights
Recombinant human c-Myc protein (Active)
- Most cited biologically active protein
- Suitable for SDS-PAGE, western blot, mass spectrometry, and functional studies
- Low endotoxin levels
- Highly rated (5* Abreviews)
Recombinant Human c-Myc protein
- One of the most cited proteins
- Human full-length protein for SDS-PAGE and western blot
Recombinant Human c-Myc protein (Tagged)
- His-tagged full-length protein
- Suitable for SDS-PAGE
Assay kits
Assessing c-Myc activation and binding activity is crucial in a research environment given its central role in regulating cell growth, differentiation, and oncogenesis. By studying how c-Myc interacts with its target genes and modulates transcriptional programs, researchers can gain insights into the molecular mechanisms driving cancer progression, metabolic reprogramming, and other cellular processes. Accurate measurement of c-Myc activity provides valuable data for understanding its contributions to disease and can help identify potential therapeutic targets. Integrating assays for transcription factor activation and chromatin interactions is essential in understanding the biological functions of c-Myc.
Product highlights
c-Myc Transcription Factor Assay Kit (Colorimetric)
- Available in a high-throughput format allowing for the simultaneous processing of multiple samples; beneficial for screening experiments or studies requiring the analysis of c-Myc activation across various conditions
- Quantify the binding activity of c-Myc to its specific DNA response elements, providing a direct measurement of transcription factor activation
- Simple and easy-to-understand readout
ChIP-Seq High Sensitivity Kit
- Enables the study of c-Myc and other transcription factors on a genome-wide scale
- Map the binding sites of c-Myc across the genome with high precision
- High signal-to-noise, enabling the detection of specific DNA-protein interactions with greater sensitivity
