AB14941
10X His tag® peptide is a Synthetic blocking peptide. >90% and suitable for BL.
View Alternative Names
Dec-His tag, Decahistidine tag, HHHHHHHHHH tag
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "BL": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
Product details
HIS-TAG® is a trademark of EMD Biosciences, Inc. - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn't dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
- If the peptide doesn't dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
Sequence info
[{"sequence":null,"proteinLength":null,"predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Synthetic","expressionSystem":null,"accessionNumber":null,"tags":[]}]
Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
Specifications
Form
Lyophilized
General info
Target data
Complementary Products
Primary Antibodies
AB237337
Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547]
RabMAb|Recombinant
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (AB237337)](https://content.abcam.com/products/images/alexa-fluor-647-6x-his-tag-antibody-epr20547-ab237337--immunocytochemistry-immunofluorescence-img109103.jpg)
- 5
- 1
![Western blot - Anti-p53 antibody [PAb 240] (AB26)](https://content.abcam.com/products/images/p53-antibody-pab-240-ab26--western-blot-img138717.jpg)
Primary Antibodies
AB109199
KO Validated|RabMAb|Recombinant|Lab Essentials
![Western blot - Anti-p21 antibody [EPR3993] (AB109199)](https://content.abcam.com/products/images/p21-antibody-epr3993-ab109199--western-blot-img181580.jpg)
- 4
- 12
Product promise
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