AB14943
10X His tag® peptide is a Synthetic blocking peptide. >90% and suitable for BL.
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "BL": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
Product details
HIS-TAG® is a trademark of EMD Biosciences, Inc. - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn't dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
- If the peptide doesn't dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
Sequence info
[{"sequence":null,"proteinLength":null,"predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Synthetic","expressionSystem":null,"accessionNumber":null,"tags":[]}]
Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
-20°C
False
Specifications
Form
Lyophilized
General info
Target data
Publications (4)
Recent publications for all applications. Explore the full list and refine your search
BMC medicine 20:462 PubMed36447243
2022
Interim analysis from a phase 2 randomized trial of EuCorVac-19: a recombinant protein SARS-CoV-2 RBD nanoliposome vaccine.
Applications
Unspecified application
Species
Unspecified reactive species
Jonathan F Lovell,Yeong Ok Baik,Seuk Keun Choi,Chankyu Lee,Jeong-Yoon Lee,Kazutoyo Miura,Wei-Chiao Huang,Young-Shin Park,Sun-Je Woo,Sang Hwan Seo,Jae-Ouk Kim,Manki Song,Chung-Jong Kim,Jae-Ki Choi,Jieun Kim,Eun Ju Choo,Jung-Hyun Choi
Nanomaterials (Basel, Switzerland) 10: PubMed32604729
2020
Detection of C-Reactive Protein Using Histag-HRP Functionalized Nanoconjugate with Signal Amplified Immunoassay.
Applications
Unspecified application
Species
Unspecified reactive species
Mohd Farhan Siddiqui,Zeeshan Ahmad Khan,Seungkyung Park
PLoS pathogens 11:e1005053 PubMed26226614
2015
Activation of TLR2 and TLR6 by Dengue NS1 Protein and Its Implications in the Immunopathogenesis of Dengue Virus Infection.
Applications
Unspecified application
Species
Unspecified reactive species
Jincheng Chen,Mary Mah-Lee Ng,Justin Jang Hann Chu
Nature communications 3:749 PubMed22434192
2012
FAT10 and NUB1L bind to the VWA domain of Rpn10 and Rpn1 to enable proteasome-mediated proteolysis.
Applications
Unspecified application
Species
Unspecified reactive species
Neha Rani,Annette Aichem,Gunter Schmidtke,Stefan G Kreft,Marcus Groettrup
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