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AB204877

Active Poly (Glu, Tyr) peptide

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(2 Publications)

Active Poly (Glu, Tyr) peptide is a Synthetic peptide. >95% and suitable for FuncS.

View Alternative Names

BRAF1, RAFB1, BRAF, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1

Key facts

Purity

>95%

Tags

Tag free

Applications

FuncS

applications

Biologically active

Yes

Biological activity

2300 nmol/min/mg Specific activity defined by radiometric assay, nmol of gamma-phosphate from ATP transferred to Unactive MEK1 and ERK1

Accession

P15056

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 0.394% Tris HCl

storage-buffer

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Ratio for molar concentration 4:1 Glu, Tyr (amount of each amino acid in the solution).
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Sequence info

[{"sequence":"","proteinLength":null,"predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Synthetic","expressionSystem":null,"accessionNumber":"P15056","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Purified by affinity chromatography

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General info

Function

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed : 21441910, PubMed : 29433126). Phosphorylates PFKFB2 (PubMed : 36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed : 1508179).

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.

Post-translational modifications

Phosphorylation at Ser-365 by SGK1 inhibits its activity (Ref.8, PubMed:11410590). Phosphorylation at Thr-753 by MAPK1 (PubMed:19710016). Dephosphorylation of Ser-365 by the SHOC2-MRAS-PP1c (SMP) complex consisting of SHOC2, GTP-bound M-Ras/MRAS and the catalytic subunit of protein phosphatase 1 (PPP1CA, PPP1CB or PPP1CC); this relieves inactivation and stimulates kinase activity (PubMed:35768504, PubMed:35830882).. Methylation at Arg-671 decreases stability and kinase activity.. Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed : 21441910, PubMed : 29433126). Phosphorylates PFKFB2 (PubMed : 36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed : 1508179).
See full target information BRAF
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Communications chemistry 5:5 PubMed36697561

2022

Structural insights into the potency and selectivity of covalent pan-FGFR inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Lingzhi Qu,Xiaojuan Chen,Hudie Wei,Ming Guo,Shuyan Dai,Longying Jiang,Jun Li,Sitong Yue,Zhuchu Chen,Yongheng Chen

Molecules (Basel, Switzerland) 23: PubMed30487406

2018

Discovery of a Natural Syk Inhibitor from Chinese Medicine through a Docking-Based Virtual Screening and Biological Assay Study.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Wang,Junfang Guo,Zhongqi Ning,Xia Wu
View all publications
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