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AB316171

AsCas12a (Active)

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AsCas12a (Active) is a Acidaminococcus sp. BV3L6 Full Length protein, in the 1 to 1307 aa range, expressed in Escherichia coli, with >95%, < 10 EU/mg endotoxin level, suitable for SDS-PAGE, Biological Activity, HPLC, FuncS.

View Alternative Names

cpf1, HMPREF1246_0236, cas12a, CRISPR-associated endonuclease Cas12a, AsCpf1, CRISPR-associated endonuclease Cpf1

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Biological Activity - AsCas12a (Active) (AB316171)
  • Biological Activity

Supplier Data

Biological Activity - AsCas12a (Active) (AB316171)

The in vitro cleavage activity data showed that AsCas12a Nuclease can target and cleave more than 95% of the substrates.

SDS-PAGE - AsCas12a (Active) (AB316171)
  • SDS-PAGE

Supplier Data

SDS-PAGE - AsCas12a (Active) (AB316171)

The purity of Recombinant Cas12a detected by Bis-Tris PAGE is greater than 95%.

HPLC - AsCas12a (Active) (AB316171)
  • HPLC

Supplier Data

HPLC - AsCas12a (Active) (AB316171)

The purity of Cas12aNuclease detected by SEC-HPLC is greater than 95%.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 10 EU/mg

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

Biological Activity, SDS-PAGE, HPLC, FuncS

applications

Biologically active

Yes

Biological activity

in vitro cleavage activity data showed that Cas12a Nuclease can target and cleave more than 95% of the substrates.

Accession

U2UMQ6

Animal free

No

Carrier free

No

Species

Acidaminococcus sp. BV3L6

Storage buffer

pH: 7.4 Constituents: 50% Glycerol (glycerin, glycerine), 1.752% Sodium chloride, 0.316% Tris HCl, 0.015% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.004% Ethylenediaminetetraacetic acid, disodium, dihydrate

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Complementary Products

Primary Antibodies

AB273438

Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7]

Recombinant|Trial Size

Western blot - Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7] (AB273438)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Biological Activity": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }
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Sequence info

[{"sequence":"MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDHYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNAIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDKFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLNLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLRNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGKITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLKKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKARNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPLEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDLSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGHHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKDQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFHVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRSLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVVVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQFTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDVKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENHRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMRNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESKDLKLQNGISNQDWLAYIQELRN","proteinLength":"Full Length","predictedMolecularWeight":"155.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":1307,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"U2UMQ6","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CRISPR-associated endonuclease Cas12a also known as Cpf1 is a single-effector protein used in genome editing. This protein has a molecular weight around 150 kDa and is found in various bacterial species as a defense mechanism against viral infections. Cas12a performs its function by creating double-strand breaks in DNA through its endonuclease activity. It recognizes specific DNA sequences via a guide RNA and cleaves the DNA allowing for precise genetic modifications.
Biological function summary

Cas12a serves an important role in the CRISPR-Cas adaptive immune system in bacteria. It is part of the Class 2 CRISPR systems and functions in a complex with a single guide RNA (sgRNA). In this complex Cas12a distinguishes itself from other CRISPR-associated proteins by requiring a simpler PAM sequence which offers greater flexibility in target DNA recognition. Cas12a's ability to cleave both strands of DNA with precision makes it a valuable tool for genetic engineering and functional genomics studies.

Pathways

Cas12a enables targeted edits in genetic pathways contributing to advancements in synthetic biology and gene therapy applications. Cas12a interacts with the DNA repair machinery influencing pathways like non-homologous end joining (NHEJ) and homology-directed repair (HDR). These pathways are also influenced by proteins such as DNA ligase IV in NHEJ and RAD51 in HDR showcasing how Cas12a-mediated DNA modifications are repaired and integrated.

Cas12a has potential applications in treating genetic disorders through targeted gene correction or disruption. Cas12a can contribute to research and therapeutic strategies for diseases like sickle cell anemia and muscular dystrophy where precise genome editing can address genetic defects. This system related to human proteins involved in DNA repair pathways may offer innovative solutions in correcting disease-causing mutations and restoring normal cellular functions.
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Specifications

Form

Liquid

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General info

Function

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Recognizes a short motif in the CRISPR repeat sequences (the 5' PAM or protospacer adjacent motif, TTTN in this organism) to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs (PubMed : 26422227). Has dsDNA endonuclease activity, results in staggered 4-base 5' overhangs 19 and 22 bases downstream of the PAM on the non-targeted and targeted strand respectively (PubMed : 26422227). Non-target strand cleavage by the RuvC domain is probably a prerequisite of target strand cleavage by the Nuc domain (PubMed : 27114038). Protects E.coli against plasmids and bacteriophage M13mp18, phage T4 with hydroxymethyl or unmodified (but not glycosylated) cytosines and to a lesser extent against lambda and VpaE1 phage (PubMed : 38261981). In this CRISPR system correct processing of pre-crRNA requires only this protein and the CRISPR locus (By similarity).

Sequence similarities

Belongs to the CRISPR-associated endonuclease Cas12a family.

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Product protocols

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Target data

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Recognizes a short motif in the CRISPR repeat sequences (the 5' PAM or protospacer adjacent motif, TTTN in this organism) to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs (PubMed : 26422227). Has dsDNA endonuclease activity, results in staggered 4-base 5' overhangs 19 and 22 bases downstream of the PAM on the non-targeted and targeted strand respectively (PubMed : 26422227). Non-target strand cleavage by the RuvC domain is probably a prerequisite of target strand cleavage by the Nuc domain (PubMed : 27114038). Protects E.coli against plasmids and bacteriophage M13mp18, phage T4 with hydroxymethyl or unmodified (but not glycosylated) cytosines and to a lesser extent against lambda and VpaE1 phage (PubMed : 38261981). In this CRISPR system correct processing of pre-crRNA requires only this protein and the CRISPR locus (By similarity).
See full target information cas12a
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