AsCas12a (Active)

Key facts

Purity

>=95% SDS-PAGE

Endotoxin level

< 10 EU/mg

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

SDS-PAGE, Biological Activity, HPLC

Biologically active

Yes

Biological activity

in vitro cleavage activity data showed that Cas12a Nuclease can target and cleave more than 95% of the substrates.

Accession

U2UMQ6-1

Animal free

No

Species

Acidaminococcus sp. BV3L6

Concentration

Storage buffer

pH: 7.4
Constituents: 50% Glycerol (glycerin, glycerine), 1.752% Sodium chloride, 0.316% Tris HCl, 0.015% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.004% Ethylenediaminetetraacetic acid, disodium, dihydrate

Sequence info

Amino acid sequence

M T Q F E G F T N L Y Q V S K T L R F E L I P Q G K T L K H I Q E Q G F I E E D K A R N D H Y K E L K P I I D R I Y K T Y A D Q C L Q L V Q L D W E N L S A A I D S Y R K E K T E E T R N A L I E E Q A T Y R N A I H D Y F I G R T D N L T D A I N K R H A E I Y K G L F K A E L F N G K V L K Q L G T V T T T E H E N A L L R S F D K F T T Y F S G F Y E N R K N V F S A E D I S T A I P H R I V Q D N F P K F K E N C H I F T R L I T A V P S L R E H F E N V K K A I G I F V S T S I E E V F S F P F Y N Q L L T Q T Q I D L Y N Q L L G G I S R E A G T E K I K G L N E V L N L A I Q K N D E T A H I I A S L P H R F I P L F K Q I L S D R N T L S F I L E E F K S D E E V I Q S F C K Y K T L L R N E N V L E T A E A L F N E L N S I D L T H I F I S H K K L E T I S S A L C D H W D T L R N A L Y E R R I S E L T G K I T K S A K E K V Q R S L K H E D I N L Q E I I S A A G K E L S E A F K Q K T S E I L S H A H A A L D Q P L P T T L K K Q E E K E I L K S Q L D S L L G L Y H L L D W F A V D E S N E V D P E F S A R L T G I K L E M E P S L S F Y N K A R N Y A T K K P Y S V E K F K L N F Q M P T L A S G W D V N K E K N N G A I L F V K N G L Y Y L G I M P K Q K G R Y K A L S F E P T E K T S E G F D K M Y Y D Y F P D A A K M I P K C S T Q L K A V T A H F Q T H T T P I L L S N N F I E P L E I T K E I Y D L N N P E K E P K K F Q T A Y A K K T G D Q K G Y R E A L C K W I D F T R D F L S K Y T K T T S I D L S S L R P S S Q Y K D L G E Y Y A E L N P L L Y H I S F Q R I A E K E I M D A V E T G K L Y L F Q I Y N K D F A K G H H G K P N L H T L Y W T G L F S P E N L A K T S I K L N G Q A E L F Y R P K S R M K R M A H R L G E K M L N K K L K D Q K T P I P D T L Y Q E L Y D Y V N H R L S H D L S D E A R A L L P N V I T K E V S H E I I K D R R F T S D K F F F H V P I T L N Y Q A A N S P S K F N Q R V N A Y L K E H P E T P I I G I D R G E R N L I Y I T V I D S T G K I L E Q R S L N T I Q Q F D Y Q K K L D N R E K E R V A A R Q A W S V V G T I K D L K Q G Y L S Q V I H E I V D L M I H Y Q A V V V L E N L N F G F K S K R T G I A E K A V Y Q Q F E K M L I D K L N C L V L K D Y P A E K V G G V L N P Y Q L T D Q F T S F A K M G T Q S G F L F Y V P A P Y T S K I D P L T G F V D P F V W K T I K N H E S R K H F L E G F D F L H Y D V K T G D F I L H F K M N R N L S F Q R G L P G F M P A W D I V F E K N E T Q F D A K G T P F I A G K R I V P V I E N H R F T G R Y R D L Y P A N E L I A L L E E K G I V F R D G S N I L P K L L E N D D S H A I D T M V A L I R S V L Q M R N S N A A T G E D Y I N S P V R D L N G V C F D S R F Q N P E W P M D A D A N G A Y H I A L K G Q L L L N H L K E S K D L K L Q N G I S N Q D W L A Y I Q E L R N

Accession

U2UMQ6

Protein length

Full Length

Predicted molecular weight

155.3 kDa

Amino acids

1 to 1307

Nature

Recombinant

Tags

His tag C-Terminus

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