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AB325718

B-CHP Collagen Hybridizing Peptide - Biotin Conjugate

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The Collagen Hybridizing Peptide (CHP) is a synthetic peptide that can specifically bind to denatured collagen strands through hydrogen bonding, both in histology, in vivo, and in vitro (3D cell culture). Additionally, CHP can be used in several biochemical assays, such as SDS-PAGE staining, for identification and quantification of collagen content in a biological sample.

B-CHP is labeled with biotin for avidin/streptavidin-mediated detection. B-CHP offers flexible detection options, such as non-green fluorescence and HRP-enzyme methods, to avoid background and enhance signal.
2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - B-CHP Collagen Hybridizing Peptide - Biotin Conjugate (AB325718)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - B-CHP Collagen Hybridizing Peptide - Biotin Conjugate (AB325718)

Immunohistochemical analysis of FFPE embedded human kidney tissues labeled with ab325718 at 20 uM. CHP detected with streptavidin HRP DAB substrate (brown). Tissues were deparaffinized following standard procedures before undergoing heat-induced epitope retrieval (HIER) to purposefully denature all collagen in the sample. Counterstained with DAPI (Blue). This section was incubated over night at 4°C.

Immunofluorescence - B-CHP Collagen Hybridizing Peptide - Biotin Conjugate (AB325718)
  • IF

Supplier Data

Immunofluorescence - B-CHP Collagen Hybridizing Peptide - Biotin Conjugate (AB325718)

Immunofluorescence (IF) analysis of FFPE embedded Rat skin tissues labeled with ab325718 at 20 uM. CHP detected with streptavidin Alexa Flour 647(magenta). Tissues were deparaffinized following standard procedures before undergoing heat-induced epitope retrieval (HIER) to purposefully denature all collagen in the sample. Counterstained with DAPI (Blue). This section was incubated over night at 37°C.

Key facts

Purity

>85% HPLC

Tags

Tag free

Applications

IHC-P, IF

applications

Biologically active

No

Conjugation

Biotin

Excitation/Emission
Animal free

Yes

Carrier free

No

Storage buffer

Constituents: 100% Proprietary Non-haz Component

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab325719 is the negative control for ab325718.

CHP binds to the unfolded triple-helical chains of all collagen types (e.g., I, II, III, IV, etc).

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Sequence info

[{"linker":null,"sequence":null,"proteinLength":null,"predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Synthetic","expressionSystem":null,"accessionNumber":null,"tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
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Specifications

Form

Lyophilized

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General info

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Product protocols

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Target data

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Product promise

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