Bovine Serum Albumin (nitrated )

• WB

Lab

Western blot - Bovine Serum Albumin (nitrated ) (AB131379)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198491 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-3-Nitrotyrosine antibody [7A12AF6] (<a href='/en-us/products/primary-antibodies/hrp-3-nitrotyrosine-antibody-7a12af6-ab198491'>ab198491</a>) at 1/5000 dilution

Lane 1:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Lane 3:

Bovine Serum Albumin at 10 µg

Lane 4:

Western blot - Bovine Serum Albumin (nitrated ) (ab131379) at 10 µg

true

Exposure time: 1s

• WB

Unknown

Western blot - Bovine Serum Albumin (nitrated ) (AB131379)

All lanes : Anti-Nitrotyrosine antibody (ab110282) 0.4 μg/mL.

Lane 1 : Molecular weight marker
Lane 2 : BSA 100 ng
Lane 3 : BSA 25 ng
Lane 4 : Nitrated BSA 100 ng
Lane 5 : Nitrated BSA 50 ng
Lane 6 : Nitrated BSA 25 ng
Lane 7 : Nitrated BSA 12.5 ng
Lane 8 : Nitrated BSA 6.25 ng
Lane 9 : Nitrated BSA 3.125 ng

Secondary antibody :
Goat polyclonal to Mouse IgG – Pre-Adsorbed (HRP) at 1/6000
developed using the ECL technique.

Performed under reducing conditions.

Predicted band size : 69,293 Da
Observed band size : 69 kDa

Exposure time : 5 minutes

All lanes:

Western blot - Bovine Serum Albumin (nitrated ) (ab131379)

false

Exposure time: 5min

• SDS-PAGE

Unknown

SDS-PAGE - Bovine Serum Albumin (nitrated ) (AB131379)

Lane 1 : Nitrated Bovine Serum Albumin protein (50 ng)
Lane 2 : Molecular weight marker
Lane 3 : Bovine Serum Albumin protein (50 ng)

Gel : 4-20% acrylamide separation gel

Predicted band size : 69 kDa