Bovine Serum Albumin (nitrated ) is a Cow Full Length protein, in the 1 to 607 aa range, expressed in Native, with >96% purity and suitable for SDS-PAGE, WB.
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Key facts
- Purity
>96% SDS-PAGE
- Expression system
Native
- Tags
Tag free
- Applications
SDS-PAGE, WB
- Biologically active
No
Amino acid sequence
M K W V T F I S L L L L F S S A Y S R G V F R R D T H K S E I A H R F K D L G E E H F K G L V L I A F S Q Y L Q Q C P F D E H V K L V N E L T E F A K T C V A D E S H A G C E K S L H T L F G D E L C K V A S L R E T Y G D M A D C C E K Q E P E R N E C F L S H K D D S P D L P K L K P D P N T L C D E F K A D E K K F W G K Y L Y E I A R R H P Y F Y A P E L L Y Y A N K Y N G V F Q E C C Q A E D K G A C L L P K I E T M R E K V L A S S A R Q R L R C A S I Q K F G E R A L K A W S V A R L S Q K F P K A E F V E V T K L V T D L T K V H K E C C H G D L L E C A D D R A D L A K Y I C D N Q D T I S S K L K E C C D K P L L E K S H C I A E V E K D A I P E N L P P L T A D F A E D K D V C K N Y Q E A K D A F L G S F L Y E Y S R R H P E Y A V S V L L R L A K E Y E A T L E E C C A K D D P H A C Y S T V F D K L K H L V D E P Q N L I K Q N C D Q F E K L G E Y G F Q N A L I V R Y T R K V P Q V S T P T L V E V S R S L G K V G T R C C T K P E S E R M P C T E D Y L S L I L N R L C V L H E K T P V S E K V T K C C T E S L V N R R P C F S A L T P D E T Y V P K A F D E K L F T F H A D I C T L P D T E K Q I K K Q T A L V E L L K H K P K A T E E Q L K T V M E N F V A F V D K C C A A D D K E A C F A V E G P K L V V S T Q T A L A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (Probable). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (PubMed:22677715). The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood (Probable). The rank order of affinity is zinc > calcium > magnesium (Probable). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).
Alternative names
Albumin, BSA, ALB
Recommended products
Bovine Serum Albumin (nitrated ) is a Cow Full Length protein, in the 1 to 607 aa range, expressed in Native, with >96% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
>96% SDS-PAGE
- Expression system
Native
- Tags
Tag free
- Applications
SDS-PAGE, WB
- Biologically active
No
- Accession
- P02769-1
- Animal free
No
- Species
Cow
- Concentration
- Storage buffer
pH: 7.5
Constituents: 15% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.61% Tris HCl
Sequence info
Amino acid sequence
M K W V T F I S L L L L F S S A Y S R G V F R R D T H K S E I A H R F K D L G E E H F K G L V L I A F S Q Y L Q Q C P F D E H V K L V N E L T E F A K T C V A D E S H A G C E K S L H T L F G D E L C K V A S L R E T Y G D M A D C C E K Q E P E R N E C F L S H K D D S P D L P K L K P D P N T L C D E F K A D E K K F W G K Y L Y E I A R R H P Y F Y A P E L L Y Y A N K Y N G V F Q E C C Q A E D K G A C L L P K I E T M R E K V L A S S A R Q R L R C A S I Q K F G E R A L K A W S V A R L S Q K F P K A E F V E V T K L V T D L T K V H K E C C H G D L L E C A D D R A D L A K Y I C D N Q D T I S S K L K E C C D K P L L E K S H C I A E V E K D A I P E N L P P L T A D F A E D K D V C K N Y Q E A K D A F L G S F L Y E Y S R R H P E Y A V S V L L R L A K E Y E A T L E E C C A K D D P H A C Y S T V F D K L K H L V D E P Q N L I K Q N C D Q F E K L G E Y G F Q N A L I V R Y T R K V P Q V S T P T L V E V S R S L G K V G T R C C T K P E S E R M P C T E D Y L S L I L N R L C V L H E K T P V S E K V T K C C T E S L V N R R P C F S A L T P D E T Y V P K A F D E K L F T F H A D I C T L P D T E K Q I K K Q T A L V E L L K H K P K A T E E Q L K T V M E N F V A F V D K C C A A D D K E A C F A V E G P K L V V S T Q T A L A
- Accession
- Protein length
Full Length
- Predicted molecular weight
69.29 kDa
- Amino acids
1 to 607
- Nature
Native
Specifications
- Form
Liquid
General info
- Function
Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (Probable). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (PubMed:22677715). The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood (Probable). The rank order of affinity is zinc > calcium > magnesium (Probable). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).
- Sequence similarities
Belongs to the ALB/AFP/VDB family.
- Post-translational modifications
Phosphorylated by FAM20C in the extracellular medium.
Storage
- Shipped at conditions
Dry Ice
- Appropriate short-term storage conditions
-80°C
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
ab131379 was treated with peroxynitrite which modifies tyrosine by acting as an oxidant to produce 3-nitrotyrosine along the length of the Bovine Serum Albumin protein.
Product was previously marketed under the MitoSciences sub-brand.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Bovine serum albumin (BSA) also known as albumin bovine serum boasts a molecular weight of approximately 66.5 kDa. This protein is found in the blood serum of cows and is derived from plasma. BSA or bovine albumin acts as a transport protein that binds to various substances including fatty acids and hormones facilitating their movement through the blood. In the lab scientists often use the BSA protein as a standard or blocking agent in experiments particularly in ELISA and Western blot assays.
- Biological function summary
Proteins such as BSA play a critical role in maintaining osmotic pressure and pH balance in biological fluids. BSA does not form part of a larger protein complex but interacts with numerous small molecules and ions stabilizing their concentrations in the bloodstream. The interaction of albumin bovine serum with fatty acids is especially important for modulating the bioavailability of lipophilic molecules.
- Pathways
Albumin actively contributes to lipid metabolism and transport pathways. It interacts closely with other proteins involved in these processes such as lipoproteins. BSA's binding affinity for various drugs and toxins also places it within the detoxification and excretory pathways ensuring the stability and solubility of these substances in circulation.
- Associated diseases and disorders
Scientists have studied BSA for its relevance to liver and kidney diseases given its role in carrying molecules and maintaining osmotic balance. Reduced levels of bovine albumin may indicate liver dysfunction or nephrotic syndrome where protein loss through urine is a concern. BSA's interaction with proteins such as globulin is also significant when investigating these conditions as the balance between these proteins can reflect the body's overall protein status.
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3 product images
Western blot - Bovine Serum Albumin (nitrated ) (ab131379)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with HRP Anti-3-Nitrotyrosine antibody [7A12AF6] ab198491 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - HRP Anti-3-Nitrotyrosine antibody [7A12AF6] (HRP Anti-3-Nitrotyrosine antibody [7A12AF6] ab198491) at 1/5000 dilution
Lane 1: Human liver tissue lysate - total protein (ab29889) at 10 µg
Lane 3: Bovine Serum Albumin at 10 µg
Lane 4: Western blot - Bovine Serum Albumin (nitrated ) (ab131379) at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1s
Western blot - Bovine Serum Albumin (nitrated ) (ab131379)
All lanes: Anti-Nitrotyrosine antibody (Anti-3-Nitrotyrosine antibody [7A12AF6] ab110282) 0.4 µg/mL.
Lane 1: Molecular weight marker
Lane 2: BSA 100 ng
Lane 3: BSA 25 ng
Lane 4: Nitrated BSA 100 ng
Lane 5: Nitrated BSA 50 ng
Lane 6: Nitrated BSA 25 ng
Lane 7: Nitrated BSA 12.5 ng
Lane 8: Nitrated BSA 6.25 ng
Lane 9: Nitrated BSA 3.125 ngSecondary antibody:
Goat polyclonal to Mouse IgG – Pre-Adsorbed (HRP) at 1/6000
developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69,293 Da
Observed band size: 69 kDa
Exposure time: 5 minutesAll lanes: Western blot - Bovine Serum Albumin (nitrated ) (ab131379)
Exposure time: 5min
SDS-PAGE - Bovine Serum Albumin (nitrated ) (ab131379)
Lane 1: Nitrated Bovine Serum Albumin protein (50 ng)
Lane 2: Molecular weight marker
Lane 3: Bovine Serum Albumin protein (50 ng)Gel: 4-20% acrylamide separation gel
Predicted band size: 69 kDa
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