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AB204854

Cdc25C peptide

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(0 Publication)

Cdc25C peptide is a Synthetic peptide. >97% and suitable for FuncS, HPLC.

View Alternative Names

M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C, CDC25C

Key facts

Purity

>97% HPLC

Tags

Tag free

Applications

HPLC, FuncS

applications

Biologically active

No

Accession

P30307

Animal free

No

Carrier free

No

Species

Human

Reconstitution

reconstitute with water at 1mg/mL

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab204854 (Cdc25C peptide) can be utilized as a substrate for the following active protein kinases:

ab139629 (Active human BRSK2 full length protein)
ab60762 (Active human Chk1 full length protein)
ab69918 (Active human Chk1 full length protein)
ab119135 (Active human MARK2 full length protein)
ab60012 (Active human Mark3 full length protein)
ab105211 (Active human MARK4 full length protein)
ab179497 (Active human Serine/threonine-protein kinase NIM1 full length protein)
ab125626 (Active human ARK5 full length protein)
ab105209 (Active human NUAK2 full length protein)

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Sequence info

[{"sequence":"KKKVSRSGLYRSPSMPENLNRPR","proteinLength":null,"predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":225,"aminoAcidStart":205,"nature":"Synthetic","expressionSystem":null,"accessionNumber":"P30307","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Lyophilized

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General info

Function

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle (PubMed : 8119945). When phosphorylated, highly effective in activating G2 cells into prophase (PubMed : 8119945). Directly dephosphorylates CDK1 and activates its kinase activity (PubMed : 8119945).

Sequence similarities

Belongs to the MPI phosphatase family.

Post-translational modifications

Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216 (PubMed:10557092). However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 occurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle (PubMed : 8119945). When phosphorylated, highly effective in activating G2 cells into prophase (PubMed : 8119945). Directly dephosphorylates CDK1 and activates its kinase activity (PubMed : 8119945).
See full target information CDC25C
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Complementary Products

Primary Antibodies

AB47322

Anti-Cdc25C (phospho S216) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25C (phospho S216) antibody (AB47322)

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Primary Antibodies

AB247671

Anti-GRK1 antibody [EPR2039(2)] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-GRK1 antibody [EPR2039(2)] - BSA and Azide free (AB247671)

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Proteins & Peptides

AB105126

Recombinant human GRK1 protein

Functional Studies - Recombinant human GRK1 protein (AB105126)

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Primary Antibodies

AB76076

Anti-HSF1 (phospho S326) antibody [EP1713Y]

RabMAb|Recombinant

Western blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (AB76076)

  • 4
  • 1

Product promise

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