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AB326316

Ebola virus-like particles (EBOV VLP)

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Ebola virus-like particles (EBOV VLP) is a Ebola virus - Mayinga, Zaire, 1976 protein, expressed in Sf9 cells, with >75%, 0.481 EU/mg endotoxin level, suitable for WB.

View Alternative Names

Matrix protein VP40, Ebola VP40, Membrane-associated protein VP40, eVP40

1 Images
Western blot - Ebola virus-like particles (EBOV VLP) (AB326316)
  • WB

Supplier Data

Western blot - Ebola virus-like particles (EBOV VLP) (AB326316)

Western Blot was used to confirm the presence of EBOV-specific GP, NP and VP40 in the VLP. VLP sample was heat-denatured under reducing condition and loaded at 1 µg/lane.

(A) GP was detected as a broadly-diffused band 60-110 kDa using 50 ng/mL of anti-EBOV GP rabbit Pab

(B) NP was detected as a single sharp band at ~90 kDa using 100 ng/mL of anti-EBOV NP rabbit Pab

(C) VP40 was detected as a single sharp band at ~40 kDa using 50 ng/mL of anti-EBOV VP40 rabbit Pab

All lanes:

Western blot - Ebola virus-like particles (EBOV VLP) (ab326316) at 1 µg

Observed band size: 60-100 kDa,90 kDa,40 kDa

false

Key facts

Purity

>75% SDS-PAGE

Endotoxin level

0.481 EU/mg

Expression system

Sf9 cells

Tags

Tag free

Applications

WB

applications

Biologically active

No

Accession

Q05128

Animal free

No

Carrier free

No

Species

Ebola virus - Mayinga, Zaire, 1976

Storage buffer

Constituents: PBS, 10% Glycerol (glycerin, glycerine), 0.9% L-Arginine, 0.7% Glutamic Acid

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1 µg", "notes":"<p></p>" } } }
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Product details

Virus-like Particles (VLP’s) expressing recombinant Ebola virus (EBOV) glycoprotein (GP), nucleoprotein (NP), and matrix protein (VP40). These VLP’s mimic Ebola virus but do not contain genetic material, thus are not infectious and make an ideal candidate as a vaccine and tool to enhance filovirus research.
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Sequence info

[{"linker":null,"sequence":"","proteinLength":null,"predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Sf9 cells","accessionNumber":"Q05128","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage duration
A few weeks
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Plays an essential role virus particle assembly and budding (PubMed : 16719918, PubMed : 33673144). Acts by interacting with viral ribonucleocapsid and host members of the ESCRT (endosomal sorting complex required for transport) system such as host VPS4, PDCD6IP/ALIX, NEDD4 or TGS101 (PubMed : 15892969, PubMed : 16719918, PubMed : 23637409, PubMed : 25786915, PubMed : 26753796, PubMed : 27489272). The interaction with host E3 ubiquitin ligase SMURF2 also facilitates virus budding (PubMed : 33673144). May play a role in immune cell dysfunction by being packaged into exosomes that can decrease the viability of recipient cells (via RNAi suppression and exosome-bystander apoptosis) (PubMed : 27872619).

Sequence similarities

Belongs to the filoviridae matrix protein VP40 family.

Post-translational modifications

Sumoylated with host SUMO1, SUMO2. Sumoylation provides stability to VP40 (PubMed:27849047). Acetylated by host EP300 in vitro (PubMed:30205953).. Ubiquitinated by host WWP1. This modification mediates efficient viral budding.

Subcellular localisation

Host late endosome membrane

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Product protocols

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Target data

Plays an essential role virus particle assembly and budding (PubMed : 16719918, PubMed : 33673144). Acts by interacting with viral ribonucleocapsid and host members of the ESCRT (endosomal sorting complex required for transport) system such as host VPS4, PDCD6IP/ALIX, NEDD4 or TGS101 (PubMed : 15892969, PubMed : 16719918, PubMed : 23637409, PubMed : 25786915, PubMed : 26753796, PubMed : 27489272). The interaction with host E3 ubiquitin ligase SMURF2 also facilitates virus budding (PubMed : 33673144). May play a role in immune cell dysfunction by being packaged into exosomes that can decrease the viability of recipient cells (via RNAi suppression and exosome-bystander apoptosis) (PubMed : 27872619).
See full target information VP40
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