EGFP protein (His tag) is a recombinant Aequorea victoria Full Length protein, in the 2 to 238 aa range, with >=97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, western blot and conjugation assays. The predicted molecular weight of ab51992 protein is 32.7 kDa.
- ideal for use as a positive control
- Contains N-terminal and C-terminal His tags
- Available in different sizes to fit your experimental needs
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View Alternative Names
Green fluorescent protein, GFP
Reactivity data
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Why is this recommended?
We recommend this product because it’s often used in the same experiment or related research.
We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.
Product details
This Enhanced GFP (eGFP) protein is suitable as control reagent for GFP expression studies. Other applications include: calibration of fluorometers and flow cytometers, fluorescence microscopy, and microinjection of GFP into cells and tissues, etc.
The excitation and emmission spectra for this ab51992 recombinant EGFP is Ex/Em = 488/509 nm.
Check out our protein gel staining guide for SDS-PAGE here
Check out our western blot protocol for more information here
This product is manufactured by BioVision, an Abcam company and was previously called 4999 Enhanced Green Fluorescent Protein (EGFP). 4999-100 is the same size as the 100 µg size of ab51992.
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Specifications
Form
Lyophilized
General info
Function
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
Sequence similarities
Belongs to the GFP family.
Post-translational modifications
Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (1)
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ACS nano 19:5240-5252 PubMed39871506
2025
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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