JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB325716

F-CHP Collagen Hybridizing Peptide - 5-FAM Conjugate

Be the first to review this product! Submit a review

|

(0 Publication)

The Collagen Hybridizing Peptide (CHP) is a synthetic peptide that can specifically bind to denatured collagen strands through hydrogen bonding, both in histology, in vivo, and in vitro (3D cell culture). Additionally, CHP can be used in several biochemical assays, such as SDS-PAGE staining, for identification and quantification of collagen content in a biological sample.

F-CHP is labeled with fluorescein for direct fluorescence detection.
2 Images
Immunofluorescence - F-CHP Collagen Hybridizing Peptide - 5-FAM Conjugate (AB325716)
  • IF

Supplier Data

Immunofluorescence - F-CHP Collagen Hybridizing Peptide - 5-FAM Conjugate (AB325716)

Immunofluorescence (IF) analysis of FFPE embedded Rat skin tissues labeled with ab325716 (green) at 20 uM. Tissues were deparaffinized following standard procedures before undergoing heat-induced epitope retrieval (HIER) to purposefully denature all collagen in the sample. This section was incubated over night at 37°C.

Immunofluorescence - F-CHP Collagen Hybridizing Peptide - 5-FAM Conjugate (AB325716)
  • IF

Supplier Data

Immunofluorescence - F-CHP Collagen Hybridizing Peptide - 5-FAM Conjugate (AB325716)

Immunofluorescence (IF) analysis of OCT embedded mouse skin tissues labeled with ab325716 (green) at 20 uM counterstained with DAPI (blue). Tissues were deparaffinized following standard procedures before undergoing heat-induced epitope retrieval (HIER) to purposefully denature all collagen in the sample. This section was incubated over night at 4°C.

Key facts

Purity

>85% HPLC

Tags

Tag free

Applications

IF

applications

Biologically active

No

Conjugation

5-FAM

Excitation/Emission

Ex: 494nm, Em: 512nm

Animal free

Yes

Carrier free

No

Storage buffer

Constituents: 100% Proprietary Non-haz Component

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

ab325717 is the negative control for ab325716.

CHP binds to the unfolded triple-helical chains of all collagen types (e.g., I, II, III, IV, etc).

style
left-column

Sequence info

[{"linker":null,"sequence":null,"proteinLength":null,"predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Synthetic","expressionSystem":null,"accessionNumber":null,"tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
style
left-column
style
left-column

Specifications

Form

Lyophilized

style
left-column

General info

style
left-column

Product protocols

style
left-column

Target data

style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com