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AB325717

F-CHP Collagen Hybridizing Peptide - 5-FAM Conjugate - Scrambled Control

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The Collagen Hybridizing Peptide (CHP) is a synthetic peptide that can specifically bind to denatured collagen strands through hydrogen bonding, both in histology, in vivo, and in vitro (3D cell culture). Additionally, CHP can be used in several biochemical assays, such as SDS-PAGE staining, for identification and quantification of collagen content in a biological sample.

F-CHP is labeled with fluorescein for direct fluorescence detection.
1 Images
Immunofluorescence - F-CHP Collagen Hybridizing Peptide - 5-FAM Conjugate - Scrambled Control (AB325717)
  • IF

Supplier Data

Immunofluorescence - F-CHP Collagen Hybridizing Peptide - 5-FAM Conjugate - Scrambled Control (AB325717)

Immunofluorescence (IF) analysis of FFPE embedded Rat skin tissues labeled with ab325717 (green) at 20 uM. Tissues were deparaffinized following standard procedures before undergoing heat-induced epitope retrieval (HIER) to purposefully denature all collagen in the sample. This section was incubated over night at 37°C.

Key facts

Purity

>85% HPLC

Tags

Tag free

Biologically active

No

Conjugation

5-FAM

Excitation/Emission

Ex: 494nm, Em: 512nm

Animal free

Yes

Carrier free

No

Storage buffer

Constituents: 100% Proprietary Non-haz Component

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IF": { "reactivity":"TESTED_AND_DOES_NOT_REACT", "dilution-info":"", "notes":"<p></p>" } } }

Product details

ab325717 is the negative control for ab325716.

CHP binds to the unfolded triple-helical chains of all collagen types (e.g., I, II, III, IV, etc).

Sequence info

[{"linker":null,"sequence":null,"proteinLength":null,"predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Synthetic","expressionSystem":null,"accessionNumber":null,"tags":[]}]

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False

Specifications

Form

Lyophilized

General info

Product protocols

Target data

Product promise

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