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AB13835

HA tag peptide

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(4 Publications)

HA tag peptide is a Synthetic blocking peptide. >90% and suitable for BL.
1 Images
Western blot - HA tag peptide (AB13835)
  • WB

Supplier Data

Western blot - HA tag peptide (AB13835)

Western blot using ab13834 on 293 cells transfected with a 55 kDa HA-tagged minigene (ab20896). Lane 1 : ab13834 Lane 2 : ab13834 with blocking peptide ab13835. Primary : Rabbit polyclonal to HA tag (1/500) Secondary : Alexa Fluor 680 Goat anti Rabbit IgG (1/5000). Lysates at 10µg/lane.

All lanes:

Western blot - Anti-HA tag antibody (<a href='/en-us/products/primary-antibodies/ha-tag-antibody-ab13834'>ab13834</a>) at 1/500 dilution

Lane 1:

HEK-293 (Human) Whole Cell Lysate - expressing HA tag protein (<a href='/en-us/products/unavailable/hek-293-human-whole-cell-lysate-expressing-ha-tag-protein-ab20896'>ab20896</a>) at 10 µg

Lane 2:

HEK-293 (Human) Whole Cell Lysate - expressing HA tag protein (<a href='/en-us/products/unavailable/hek-293-human-whole-cell-lysate-expressing-ha-tag-protein-ab20896'>ab20896</a>) at 10 µg with HA tag peptide (ab13835)

Secondary

All lanes:

Alexa Fluor 680 Goat-anti-Rabbit IgG at 1/5000 dilution

Predicted band size: 64 kDa

Observed band size: 55 kDa

false

Key facts

Purity

>90% HPLC

Tags

Tag free

Applications

BL

applications

Biologically active

No

Animal free

Yes

Carrier free

No

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "BL": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn't dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Sequence info

[{"sequence":null,"proteinLength":null,"predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Synthetic","expressionSystem":null,"accessionNumber":null,"tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
-20°C
False
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Specifications

Form

Lyophilized

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General info

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Product protocols

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Target data

See full target information HA tag
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37047592

2023

NF-κB Transcriptional Activity Indispensably Mediates Hypoxia-Reoxygenation Stress-Induced microRNA-210 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Gurdeep Marwarha,Katrine Hordnes Slagsvold,Morten Andre Høydal

International journal of molecular sciences 23: PubMed36012628

2022

GSK3β Inhibition Is the Molecular Pivot That Underlies the Mir-210-Induced Attenuation of Intrinsic Apoptosis Cascade during Hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Gurdeep Marwarha,Øystein Røsand,Katrine Hordnes Slagsvold,Morten Andre Høydal

Cell reports 23:3340-3351.e5 PubMed29898403

2018

Arabidopsis Duodecuple Mutant of PYL ABA Receptors Reveals PYL Repression of ABA-Independent SnRK2 Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhao,Zhengjing Zhang,Jinghui Gao,Pengcheng Wang,Tao Hu,Zegang Wang,Yueh-Ju Hou,Yizhen Wan,Wenshan Liu,Shaojun Xie,Tianjiao Lu,Liang Xue,Yajie Liu,Alberto P Macho,W Andy Tao,Ray A Bressan,Jian-Kang Zhu

Proceedings of the National Academy of Sciences of 113:1949-54 PubMed26831097

2016

ABA receptor PYL9 promotes drought resistance and leaf senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhao,Zhulong Chan,Jinghui Gao,Lu Xing,Minjie Cao,Chunmei Yu,Yuanlei Hu,Jun You,Haitao Shi,Yingfang Zhu,Yuehua Gong,Zixin Mu,Haiqing Wang,Xin Deng,Pengcheng Wang,Ray A Bressan,Jian-Kang Zhu
View all publications
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Product promise

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