HA tag peptide is a Synthetic blocking peptide. >90% purity and suitable for BL.
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- International journal of molecular sciences 24:2023NF-κB Transcriptional Activity Indispensably Mediates Hypoxia-Reoxygenation Stress-Induced microRNA-210 Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGurdeep Marwarha,Katrine Hordnes Slagsvold,Morten Andre HøydalPubMed 37047592
- International journal of molecular sciences 23:2022GSK3β Inhibition Is the Molecular Pivot That Underlies the Mir-210-Induced Attenuation of Intrinsic Apoptosis Cascade during Hypoxia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGurdeep Marwarha,Øystein Røsand,Katrine Hordnes Slagsvold,Morten Andre HøydalPubMed 36012628
- Cell reports 23:3340-3351.e52018Arabidopsis Duodecuple Mutant of PYL ABA Receptors Reveals PYL Repression of ABA-Independent SnRK2 Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Zhao,Zhengjing Zhang,Jinghui Gao,Pengcheng Wang,Tao Hu,Zegang Wang,Yueh-Ju Hou,Yizhen Wan,Wenshan Liu,Shaojun Xie,Tianjiao Lu,Liang Xue,Yajie Liu,Alberto P Macho,W Andy Tao,Ray A Bressan,Jian-Kang ZhuPubMed 29898403
- Proceedings of the National Academy of Sciences of 113:1949-542016ABA receptor PYL9 promotes drought resistance and leaf senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Zhao et. al.PubMed 26831097
Key facts
- Purity
- >90% HPLC
- Tags
- Tag free
- Applications
- BL
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application BL | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
HA epitope tag, HA2, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain
Recommended products
HA tag peptide is a Synthetic blocking peptide. >90% purity and suitable for BL.
Key facts
- Purity
- >90% HPLC
- Tags
- Tag free
- Applications
- BL
- Biologically active
- No
- Animal free
- Yes
- Concentration
Specifications
- Form
- Lyophilized
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- -20°C
Notes
- If the peptide doesn't dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - HA tag peptide (ab13835)
Western blot using Anti-HA tag antibody ab13834 on 293 cells transfected with a 55 kDa HA-tagged minigene (ab20896).
Lane 1: Anti-HA tag antibody ab13834
Lane 2: Anti-HA tag antibody ab13834 with blocking peptide ab13835.
Primary: Rabbit polyclonal to HA tag (1/500)
Secondary: Alexa Fluor 680 Goat anti Rabbit IgG (1/5000).
Lysates at 10µg/lane.All lanes: Western blot - Anti-HA tag antibody (Anti-HA tag antibody ab13834) at 1/500 dilution
All lanes: HEK-293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896) at 10 µg
SecondaryAll lanes: Alexa Fluor 680 Goat-anti-Rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 55 kDa
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Product protocols
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