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HIV1 gp120 + gp41 peptide is a Synthetic peptide. >90% purity and suitable for WB, ELISA.

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Key facts

Purity

>90% SDS-PAGE

Tags

Tag free

Applications

WB, ELISA

Biologically active

No

Reactivity data

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

-

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Envelope glycoprotein gp160Oligomerizes in the host endoplasmic reticulum into predominantly trimers. In a second time, gp160 transits in the host Golgi, where glycosylation is completed. The precursor is then proteolytically cleaved in the trans-Golgi and thereby activated by cellular furin or furin-like proteases to produce gp120 and gp41.Surface protein gp120Attaches the virus to the host lymphoid cell by binding to the primary receptor CD4. This interaction induces a structural rearrangement creating a high affinity binding site for a chemokine coreceptor like CXCR4 and/or CCR5. Acts as a ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on endothelial cells of liver sinusoids and lymph node sinuses. These interactions allow capture of viral particles at mucosal surfaces by these cells and subsequent transmission to permissive cells. HIV subverts the migration properties of dendritic cells to gain access to CD4+ T-cells in lymph nodes. Virus transmission to permissive T-cells occurs either in trans (without DCs infection, through viral capture and transmission), or in cis (following DCs productive infection, through the usual CD4-gp120 interaction), thereby inducing a robust infection. In trans infection, bound virions remain infectious over days and it is proposed that they are not degraded, but protected in non-lysosomal acidic organelles within the DCs close to the cell membrane thus contributing to the viral infectious potential during DCs' migration from the periphery to the lymphoid tissues. On arrival at lymphoid tissues, intact virions recycle back to DCs' cell surface allowing virus transmission to CD4+ T-cells.Transmembrane protein gp41Acts as a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During fusion of viral and target intracellular membranes, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Complete fusion occurs in host cell endosomes and is dynamin-dependent, however some lipid transfer might occur at the plasma membrane. The virus undergoes clathrin-dependent internalization long before endosomal fusion, thus minimizing the surface exposure of conserved viral epitopes during fusion and reducing the efficacy of inhibitors targeting these epitopes. Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm.

Alternative names

Recommended products

HIV1 gp120 + gp41 peptide is a Synthetic peptide. >90% purity and suitable for WB, ELISA.

Key facts

Purity

>90% SDS-PAGE

Applications

WB, ELISA

Accession
P04578-1
Animal free

No

Species

HIV-1 M:B_HXB2R

Concentration
Loading...
Storage buffer

pH: 7.2 - 7.6
Constituents: 0.16% Tris HCl, 0.06% EDTA

Sequence info

Amino acid sequence

Accession

P04578

Nature

Synthetic

Specifications

Form

Liquid

General info

Function

Envelope glycoprotein gp160

Sequence similarities

Belongs to the HIV-1 env protein family.

Post-translational modifications

Palmitoylation of the transmembrane protein and of Env polyprotein (prior to its proteolytic cleavage) is essential for their association with host cell membrane lipid rafts. Palmitoylation is therefore required for envelope trafficking to classical lipid rafts, but not for viral replication.

Subcellular localisation

Host endosome membrane

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Detects many HIV-type O infected individuals responding to HIV envelope antibodies.

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