JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB326357

Human CD20/MS4A1 VLP

Be the first to review this product! Submit a review

|

(0 Publication)

Human CD20/MS4A1 VLP is a Human protein, in the 1 to 297 aa range, expressed in HEK 293 cells, suitable for ELISA, HPLC.

View Alternative Names

CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1

4 Images
ELISA - Human CD20/MS4A1 VLP (AB326357)
  • ELISA

Supplier Data

ELISA - Human CD20/MS4A1 VLP (AB326357)

Immobilized Human CD20 VLP at 5ug/ml (100ul/Well) on plate on the plate. Dose response curve for Rituximab, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA.

ELISA - Human CD20/MS4A1 VLP (AB326357)
  • ELISA

Supplier Data

ELISA - Human CD20/MS4A1 VLP (AB326357)

Immobilized Human Human CD20 VLP at 5ug/ml (100ul/well) on the plate. Dose response curve for Obinutuzumab, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.

ELISA - Human CD20/MS4A1 VLP (AB326357)
  • ELISA

Supplier Data

ELISA - Human CD20/MS4A1 VLP (AB326357)

Immobilized Human Human CD20 VLP at 5ug/ml (100ul/well) on the plate. Dose response curve for Ofatumumab, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.

HPLC - Human CD20/MS4A1 VLP (AB326357)
  • HPLC

Supplier Data

HPLC - Human CD20/MS4A1 VLP (AB326357)

The purity of Human CD20 VLP is greater than 95% as determined by SEC-HPLC.

Key facts

Expression system

HEK 293 cells

Tags

Tag free

Applications

HPLC, ELISA

applications

Biologically active

Yes

Biological activity

Immobilized Human CD20 VLP at 5ug/ml (100ul/Well) on plate on the plate. Dose response curve for Rituximab, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA (QC Test).

Conjugation

Biotin

Excitation/Emission
Accession

P11836

Animal free

No

Carrier free

No

Species

Human

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"5 µg/mL", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Recombinant Human CD20/MS4A1 VLP is expressed from HEK293.It contains Met1-Pro297 (It may have cross reaction with anti-His antibody).

B-lymphocyte antigen CD20 or CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R , CD117) and progressively increasing in concentration until maturity.CD20 is the target of the monoclonal antibodies rituximab, ocrelizumab, obinutuzumab, ofatumumab, ibritumomab tiuxetan, tositumomab, and ublituximab, which are all active agents in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases.

style
left-column

Sequence info

[{"sequence":"MTTPRNSVNGTFPAEPMKGPIAMQSGPKPLFRRMSSLVGPTQSFFMRESKTLGAVQIMNGLFHIALGGLLMIPAGIYAPICVTVWYPLWGGIMYIISGSLLAATEKNSRKCLVKGKMIMNSLSLFAAISGMILSIMDILNIKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQELVIAGIVENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP","proteinLength":null,"predictedMolecularWeight":"34.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":297,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P11836","tags":[]}]
style
left-column

Properties and storage information

True
style
left-column
style
left-column

General info

Function

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).

Sequence similarities

Belongs to the MS4A family.

Post-translational modifications

Phosphorylated on serines and threonines in resting B-cells. Protein kinase C/PKC can use CD20 as substrate.

style
left-column

Product protocols

style
left-column

Target data

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739).
See full target information MS4A1
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com