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AB101725

Human Collagen III peptide

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Human Collagen III peptide is a Synthetic blocking peptide. >70% and suitable for BL.

View Alternative Names

Collagen alpha-1(III) chain, COL3A1

Key facts

Purity

>70% HPLC

Tags

Tag free

Applications

BL

applications

Biologically active

No

Accession

P02461

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 6.75 Constituents: 0.87% Sodium chloride, 0.714% HEPES, 0.0584% EDTA, 0.001% Sorbitan monolaurate, ethoxylated

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "BL": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn't dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Sequence info

[{"sequence":null,"proteinLength":null,"predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Synthetic","expressionSystem":null,"accessionNumber":"P02461","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

70 - 90% by HPLC

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General info

Function

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.

Sequence similarities

Belongs to the fibrillar collagen family.

Post-translational modifications

Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.. O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.

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Product protocols

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Target data

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
See full target information COL3A1
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Alternative Products

Proteins & Peptides

AB196093

Recombinant Mouse Collagen III protein (His tag)

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AB7528

Native Cow Collagen III protein

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Proteins & Peptides

AB7535

Native Human Collagen III protein

SDS-PAGE - Native Human Collagen III protein (AB7535)

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Complementary Products

Assay Kits

AB222942

Total Collagen Assay Kit (Perchlorate-Free)

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Proteins & Peptides

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Proteins & Peptides

AB314201

Native Bovine Collagen protein (FITC)

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Primary Antibodies

AB184993

Anti-Collagen III antibody [EPR17673]

RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (AB184993)

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Primary Antibodies

AB260043

Anti-Collagen I antibody [EPR22894-89]

RabMAb|Recombinant|Lab Essentials

Western blot - Anti-Collagen I antibody [EPR22894-89] (AB260043)

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