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Human Histone H3 (acetyl K27) peptide is a Synthetic blocking peptide. >90% purity and suitable for Dot, BL.


Images

Western blot - Human Histone H3 (acetyl K27) peptide (AB24404), expandable thumbnail
  • Dot Blot - Human Histone H3 (acetyl K27) peptide (AB24404), expandable thumbnail
  • Western blot - Human Histone H3 (acetyl K27) peptide (AB24404), expandable thumbnail
  • Western blot - Human Histone H3 (acetyl K27) peptide (AB24404), expandable thumbnail
  • Western blot - Human Histone H3 (acetyl K27) peptide (AB24404), expandable thumbnail

Publications

Key facts

Purity
>90% HPLC
Tags
Tag free
Applications
Dot, BL
Biologically active
No

Reactivity data

Application
Dot
Reactivity
Reacts
Dilution info
-
Notes

-

Application
BL
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Alternative names

Recommended products

Human Histone H3 (acetyl K27) peptide is a Synthetic blocking peptide. >90% purity and suitable for Dot, BL.

Key facts

Purity
>90% HPLC
Applications
Dot, BL
Accession
P68431-1
Animal free
No
Species
Human
Concentration
Loading...
Storage buffer

pH: 6.75
Constituents: 0.87% Sodium chloride, 0.714% HEPES, 0.0584% EDTA, 0.001% Tween

Sequence info

Amino acid sequence

Accession
P68431
Nature
Synthetic

Specifications

Form
Liquid

General info

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Sequence similarities

Belongs to the histone H3 family.

Post-translational modifications

Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.

Subcellular localisation
Nucleus

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn't dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Human Histone H3 (acetyl K27) peptide (ab24404), expandable thumbnail

    Western blot - Human Histone H3 (acetyl K27) peptide (ab24404)

    All lanes: Anti-Histone H3 (acetyl K23) antibody (ab47813) at 1 µg/mL

    Lanes 1, 4, 7 and 8: HeLa Histone Preparation Nuclear Lysate - Butyrated at 2.5 µg

    Lane 2: HeLa Histone Preparation Nuclear Lysate - Butyrated at 2.5 µg with Human Histone H3 peptide (ab17163)

    Lane 3: HeLa Histone Preparation Nuclear Lysate - Butyrated at 2.5 µg with Histone H3 peptide - acetyl K36

    Lane 5: HeLa Histone Preparation Nuclear Lysate - Butyrated at 2.5 µg with Histone H3 peptide - acetyl K14

    Lane 6: HeLa Histone Preparation Nuclear Lysate - Butyrated at 2.5 µg with Human Histone H3 (acetyl K18) peptide (ab24003)

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

  • Dot Blot - Human Histone H3 (acetyl K27) peptide (ab24404), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Dot Blot - Human Histone H3 (acetyl K27) peptide (ab24404)

    ab24404 (at 0.01 and 0.1 μg) used in Dot Blot to test for cross-reactivity of Anti-Histone H3 (acetyl K27) antibody - ChIP Grade ab4729 (1/2500). An HRP-conjugated Mouse anti-rabbit polyclonal (1/10000) was used as the secondary antibody.

  • Western blot - Human Histone H3 (acetyl K27) peptide (ab24404), expandable thumbnail

    Western blot - Human Histone H3 (acetyl K27) peptide (ab24404)

    All lanes: Western blot - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (Anti-Histone H3 (acetyl K9) antibody - ChIP Grade ab10812) at 1 µg/mL

    Lanes 1, 2, 3, 6 and 7: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

    Lane 4: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Histone H3 peptide - acetyl K14

    Lane 5: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (acetyl K18) peptide (ab24003)

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa

    Observed band size: 17 kDa

    Exposure time: 30s

  • Western blot - Human Histone H3 (acetyl K27) peptide (ab24404), expandable thumbnail

    Western blot - Human Histone H3 (acetyl K27) peptide (ab24404)

    All lanes: Western blot - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (Anti-Histone H3 (acetyl K18) antibody - ChIP Grade ab1191) at 1 µg/mL

    Lanes 1, 3, 6, 7 and 9: HeLa Histone Preparation Nuclear Lysate at 2.5 µg

    Lane 2: HeLa Histone Preparation Nuclear Lysate at 2.5 µg with Histone H3 peptide - unmodified R17

    Lane 4: HeLa Histone Preparation Nuclear Lysate at 2.5 µg with Histone H3 peptide - acetyl K14

    Lane 5: HeLa Histone Preparation Nuclear Lysate at 2.5 µg with Human Histone H3 (acetyl K18) peptide (ab24003)

    Lane 8: HeLa Histone Preparation Nuclear Lysate at 2.5 µg with Histone H3 peptide - acetyl K36

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa

    Observed band size: 17 kDa

    Exposure time: 1min

  • Western blot - Human Histone H3 (acetyl K27) peptide (ab24404), expandable thumbnail

    Western blot - Human Histone H3 (acetyl K27) peptide (ab24404)

    Anti-Histone H3 (acetyl K27) antibody - ChIP Grade ab4729 specifically recognises acetyl K27 histone H3 in catlf thymus histone lysate, which is specifically blocked using the immunizing peptide ab24404.

    Lanes 1 and 3: Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (Anti-Histone H3 (acetyl K27) antibody - ChIP Grade ab4729) at 0.2 µg/mL

    Lanes 2 and 4: Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (Anti-Histone H3 (acetyl K27) antibody - ChIP Grade ab4729) at 0.1 µg/mL

    All lanes: Calf thymus histone lysate at 1 µg

    Secondary

    All lanes: Goat anti-rabbit (HRP) at 1/2000 dilution

    Predicted band size: 15 kDa

    Observed band size: 17 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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