Human Histone H3 (acetyl K27) peptide is a Synthetic blocking peptide. >90% purity and suitable for Dot, BL.
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Publications
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- Plant science : an international journal of experi 276:250-2672018Epigenetic and transcriptional control of chickpea WRKY40 promoter activity under Fusarium stress and its heterologous expression in Arabidopsis leads to enhanced resistance against bacterial pathogen.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoydeep Chakraborty et. al.PubMed 30348325
Key facts
- Purity
- >90% HPLC
- Tags
- Tag free
- Applications
- Dot, BL
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Application BL | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3FC, HIST1H3C, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K27ac
Recommended products
Human Histone H3 (acetyl K27) peptide is a Synthetic blocking peptide. >90% purity and suitable for Dot, BL.
Key facts
- Purity
- >90% HPLC
- Tags
- Tag free
- Applications
- Dot, BL
- Biologically active
- No
- Accession
- P68431-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 6.75
Constituents: 0.87% Sodium chloride, 0.714% HEPES, 0.0584% EDTA, 0.001% Tween
Specifications
- Form
- Liquid
General info
- Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
- Sequence similarities
Belongs to the histone H3 family.
- Post-translational modifications
Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
- If the peptide doesn't dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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5 product images
Western blot - Human Histone H3 (acetyl K27) peptide (ab24404)
All lanes: Anti-Histone H3 (acetyl K23) antibody (ab47813) at 1 µg/mL
Lanes 1, 4, 7 and 8: HeLa Histone Preparation Nuclear Lysate - Butyrated at 2.5 µg
Lane 2: HeLa Histone Preparation Nuclear Lysate - Butyrated at 2.5 µg with Human Histone H3 peptide (ab17163)
Lane 3: HeLa Histone Preparation Nuclear Lysate - Butyrated at 2.5 µg with Histone H3 peptide - acetyl K36
Lane 5: HeLa Histone Preparation Nuclear Lysate - Butyrated at 2.5 µg with Histone H3 peptide - acetyl K14
Lane 6: HeLa Histone Preparation Nuclear Lysate - Butyrated at 2.5 µg with Human Histone H3 (acetyl K18) peptide (ab24003)
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
This image is courtesy of an anonymous customer reviewDot Blot - Human Histone H3 (acetyl K27) peptide (ab24404)
ab24404 (at 0.01 and 0.1 μg) used in Dot Blot to test for cross-reactivity of Anti-Histone H3 (acetyl K27) antibody - ChIP Grade ab4729 (1/2500). An HRP-conjugated Mouse anti-rabbit polyclonal (1/10000) was used as the secondary antibody.
Western blot - Human Histone H3 (acetyl K27) peptide (ab24404)
All lanes: Western blot - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (Anti-Histone H3 (acetyl K9) antibody - ChIP Grade ab10812) at 1 µg/mL
Lanes 1, 2, 3, 6 and 7: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
Lane 4: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Histone H3 peptide - acetyl K14
Lane 5: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (acetyl K18) peptide (ab24003)
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 30s
Western blot - Human Histone H3 (acetyl K27) peptide (ab24404)
All lanes: Western blot - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (Anti-Histone H3 (acetyl K18) antibody - ChIP Grade ab1191) at 1 µg/mL
Lanes 1, 3, 6, 7 and 9: HeLa Histone Preparation Nuclear Lysate at 2.5 µg
Lane 2: HeLa Histone Preparation Nuclear Lysate at 2.5 µg with Histone H3 peptide - unmodified R17
Lane 4: HeLa Histone Preparation Nuclear Lysate at 2.5 µg with Histone H3 peptide - acetyl K14
Lane 5: HeLa Histone Preparation Nuclear Lysate at 2.5 µg with Human Histone H3 (acetyl K18) peptide (ab24003)
Lane 8: HeLa Histone Preparation Nuclear Lysate at 2.5 µg with Histone H3 peptide - acetyl K36
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 1min
Western blot - Human Histone H3 (acetyl K27) peptide (ab24404)
Anti-Histone H3 (acetyl K27) antibody - ChIP Grade ab4729 specifically recognises acetyl K27 histone H3 in catlf thymus histone lysate, which is specifically blocked using the immunizing peptide ab24404.
Lanes 1 and 3: Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (Anti-Histone H3 (acetyl K27) antibody - ChIP Grade ab4729) at 0.2 µg/mL
Lanes 2 and 4: Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (Anti-Histone H3 (acetyl K27) antibody - ChIP Grade ab4729) at 0.1 µg/mL
All lanes: Calf thymus histone lysate at 1 µg
SecondaryAll lanes: Goat anti-rabbit (HRP) at 1/2000 dilution
Predicted band size: 15 kDa
Observed band size: 17 kDa
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