AB1772

Human Histone H3 (di methyl K9) peptide

Name: Human Histone H3K9me2 peptide (ab1772) | Abcam
Brand: Abcam Limited
SKU: AB1772
Price: 435 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(9 Publications)

Human Histone H3 (di methyl K9) peptide is a Synthetic blocking peptide. >90% and suitable for BL.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K9me2, H3K9me, H3K9

17 Images

ChIP - Human Histone H3 (di methyl K9) peptide (AB1772)

ChIP

Project7459****

ChIP - Human Histone H3 (di methyl K9) peptide (AB1772)

Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab78513 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

Lab

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

Lane 8 shows that Rabbit polyclonal to Histone H3 (tri methyl K9) is blocked by the addition of the immunizing peptide (ab1773). Cross-reactivity with Histone H3 peptide - tri methyl K27 (ab1782) is also shown in Lane 11.

All lanes:

Western blot - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-tri-methyl-k9-antibody-chip-grade-ab8898'>ab8898</a>) at 1 µg/mL

All lanes:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Unknown

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

The peptide competition assay shows that this antibody is very specific for Histone H3 di-methylated at K27. It does not recognise Histone H3 mono, di, or tri methylated at K4, Histone H3 mono, di, or tri methylated at K9, or Histone H3 mono, or tri methylated at K27.

All lanes:

Western blot - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-di-methyl-k27-antibody-chip-grade-ab24684'>ab24684</a>) at 1 µg/mL

Lanes 1, 3, 4, 5, 6, 7, 8, 9, 10 and 11:

Calf thymus histone lysate

Lane 2:

Calf thymus histone lysate with Human Histone H3 peptide (<a href='/en-us/products/unavailable/human-histone-h3-peptide-ab17163'>ab17163</a>)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG H&L (HRP) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Exposure time: 5s

Project5783****

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

All lanes:

Western blot - Anti-Histone H4 (mono methyl K20) antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h4-mono-methyl-k20-antibody-chip-grade-ab9051'>ab9051</a>) at 1 µg/mL

Lanes 1, 3, 4, 5, 8, 9, 10 and 11:

Calf Thymus Histone Preparation Nuclear Lysate (<a href='/en-us/products/unavailable/calf-thymus-histone-preparation-nuclear-lysate-ab121'>ab121</a>) at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate (<a href='/en-us/products/unavailable/calf-thymus-histone-preparation-nuclear-lysate-ab121'>ab121</a>) at 0.5 µg with Human Histone H4 peptide (<a href='/en-us/products/unavailable/human-histone-h4-peptide-ab14963'>ab14963</a>)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate (<a href='/en-us/products/unavailable/calf-thymus-histone-preparation-nuclear-lysate-ab121'>ab121</a>) at 0.5 µg with Human Histone H4 (mono methyl K20) peptide (<a href='/en-us/products/unavailable/human-histone-h4-mono-methyl-k20-peptide-ab17043'>ab17043</a>)

Lane 7:

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 11 kDa

Observed band size: 13 kDa

false

Exposure time: 10s

Lab

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab78517).

ab78517 is specific for Histone H4 (tri-methyl K20). This is illustrated in lane 8 where the activity of ab78517 is specifically blocked by the addition of the immunizing peptide (ab17567).

All lanes:

Western blot - Anti-Histone H4 (di methyl K20, tri methyl K20) antibody [6F8-D9] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/histone-h4-di-methyl-k20-tri-methyl-k20-antibody-6f8-d9-bsa-and-azide-free-ab264528'>ab264528</a>) at 1 µg/mL

Lanes 1, 3, 4, 5, 9, 10 and 11:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H4 peptide (<a href='/en-us/products/unavailable/human-histone-h4-peptide-ab14963'>ab14963</a>)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H4 (mono methyl K20) peptide (<a href='/en-us/products/unavailable/human-histone-h4-mono-methyl-k20-peptide-ab17043'>ab17043</a>)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Histone H4 peptide (17-24) - di methyl K20

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 11 kDa

Observed band size: 13 kDa

true

Exposure time: 90s

Project8270****

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine, and sodium azide (ab12209).

All lanes:

Western blot - Anti-Histone H3 (tri methyl K4) antibody [mAbcam12209] - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-tri-methyl-k4-antibody-mabcam12209-chip-grade-ab12209'>ab12209</a>) at 2 µg/mL

All lanes:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Exposure time: 16min

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

All lanes:

Western blot - HRP Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] (<a href='/en-us/products/primary-antibodies/hrp-histone-h3-di-methyl-k9-antibody-mabcam-1220-ab62168'>ab62168</a>) at 1/20000 dilution

All lanes:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Lab

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab6147 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (<a href='/en-us/products/primary-antibodies/histone-h3-di-methyl-k27-tri-methyl-k27-antibody-mabcam-6147-ab6147'>ab6147</a>) at 1 µg/mL

All lanes:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

true

Exposure time: 1min

Project7459****

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

ab78513 is blocked by the addition of Histone H4 (mono methyl K20)peptide (ab17043; lane 6). ab78513 is partially blocked by Histone H4 (di methyl K20) peptide (lane 7).

All lanes:

Western blot - Anti-Histone H4 (mono methyl K20) antibody [5E10-D8] - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h4-mono-methyl-k20-antibody-5e10-d8-chip-grade-ab78513'>ab78513</a>) at 1 µg/mL

Lanes 1, 3, 4, 5, 8, 9, 10 and 11:

Calf Thymus Histone Preparation Nuclear Lysate (<a href='/en-us/products/unavailable/calf-thymus-histone-preparation-nuclear-lysate-ab121'>ab121</a>) at 0.5 µg

Lane 2:

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate (<a href='/en-us/products/unavailable/calf-thymus-histone-preparation-nuclear-lysate-ab121'>ab121</a>) at 0.5 µg with Human Histone H4 (mono methyl K20) peptide (<a href='/en-us/products/unavailable/human-histone-h4-mono-methyl-k20-peptide-ab17043'>ab17043</a>)

Lane 7:

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 11 kDa

Observed band size: 13 kDa,17 kDa

true

Exposure time: 1min

Lab

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

ab78517 is specific for Histone H4 (tri-methyl K20). This is illustrated in lane 8 where the activity of ab78517 is specifically blocked by the addition of the immunizing peptide (ab17567).

All lanes:

Western blot - Anti-Histone H4 (di methyl K20, tri methyl K20) antibody [6F8-D9] (<a href='/en-us/products/primary-antibodies/histone-h4-di-methyl-k20-tri-methyl-k20-antibody-6f8-d9-ab78517'>ab78517</a>) at 1 µg/mL

Lanes 1, 3, 4, 5, 8, 9, 10 and 11:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H4 peptide (<a href='/en-us/products/unavailable/human-histone-h4-peptide-ab14963'>ab14963</a>)

Lane 6:

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Histone H4 peptide (17-24) - di methyl K20

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 11 kDa

Observed band size: 13 kDa

true

Exposure time: 90s

Project3455****

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

All lanes:

Western blot - Anti-Histone H3 (tri methyl K79) antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-tri-methyl-k79-antibody-chip-grade-ab2621'>ab2621</a>) at 1 µg/mL

Lanes 1 and 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified ) peptide (<a href='/en-us/products/unavailable/human-histone-h3-unmodified-peptide-ab4558'>ab4558</a>)

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K79) peptide (<a href='/en-us/products/unavailable/human-histone-h3-mono-methyl-k79-peptide-ab4555'>ab4555</a>)

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K79) peptide (<a href='/en-us/products/unavailable/human-histone-h3-di-methyl-k79-peptide-ab4556'>ab4556</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K79) peptide (<a href='/en-us/products/unavailable/human-histone-h3-tri-methyl-k79-peptide-ab4557'>ab4557</a>)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H4 (di methyl K79) peptide (<a href='/en-us/products/unavailable/human-histone-h4-di-methyl-k79-peptide-ab4560'>ab4560</a>)

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Project

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

All lanes:

Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-mono-methyl-k9-antibody-chip-grade-ab9045'>ab9045</a>) at 1 µg/mL

All lanes:

Calf Thymus Histone Preparation Nuclear Lysate (<a href='/en-us/products/unavailable/calf-thymus-histone-preparation-nuclear-lysate-ab121'>ab121</a>) at 0.5 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 15 kDa

false

Project

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

ab8896 specifically recognises mono-methyl K9 (lane 1) in calf thymus lysate at 17kDa. ab8896 is successfully blocked using the immunizing peptide (lane 2 ab1771), but not the di-methyl K9 (lane 3 ab1772), the tri-methyl K9 (lane 4 ab1773), the mono-methyl K27 (lane 5 ab1780), the di-methyl K27 (lane 6 ab1781), the tri-methyl K27 (lane 7 ab1782), the mono-methyl K4 (lane 8 ab1340) nor the corresponding unmodified peptides (lane 9 ab7228, lane 10 ab2623). This implies that ab8896 is specific to mono-methyl K9.

All lanes:

Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-mono-methyl-k9-antibody-chip-grade-ab8896'>ab8896</a>) at 1 µg/mL

All lanes:

Calf Thymus Histone Preparation at 0.5 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Project1016****

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

All lanes:

Western blot - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-di-methyl-k36-antibody-chip-grade-ab9049'>ab9049</a>) at 1 µg/mL

Lanes 1, 4, 5, 6, 7, 8, 9, 10 and 11:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Histone H3 peptide (ab41430)

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K36) peptide (<a href='/en-us/products/unavailable/human-histone-h3-mono-methyl-k36-peptide-ab1783'>ab1783</a>)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Unknown

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

All lanes:

Western blot - Anti-Histone H3 (di methyl K4, tri methyl K4) antibody [mAbcam 6000] - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-di-methyl-k4-tri-methyl-k4-antibody-mabcam-6000-chip-grade-ab6000'>ab6000</a>) at 1 µg/mL

All lanes:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/10000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

true

Exposure time: 20min

Project1592****

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

Expected molecular weights : H3 = 17 kDa; H4 = 14 kDa This image shows that the main epitopes recognized by ab7315 are the di methylated lysine residues. This can be seen in lanes 4, 7 and 10 where the activity of the antibody is quenched by the immunizing peptides (ab7768, ab1772, ab1781).

All lanes:

Western blot - Anti-pan methyl Lysine antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/pan-methyl-lysine-antibody-chip-grade-ab7315'>ab7315</a>) at 1 µg/mL

All lanes:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 14-17 kDa

true

Exposure time: 20min

Project

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

All lanes : Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)

Lane 1 : Calf thymus histone lysate
Lane 2 : Calf thymus histone lysate with Histone H3 peptide - unmodified at 1 μg/ml
Lane 3 : Calf thymus histone lysate with Human Histone H3 (mono methyl K9) peptide (ab1771) at 1 μg/ml
Lane 4 : Calf thymus histone lysate with Human Histone H3 (di methyl K9) peptide (ab1772) at 1 μg/ml
Lane 5 : Calf thymus histone lysate with Human Histone H3 (tri methyl K9) peptide (ab1773) at 1 μg/ml
Lane 6 : Calf thymus histone lysate with Histone H3 (di methyl K4) peptide (ab7768) at 1 μg/ml
Lane 7 : Calf thymus histone lysate with Human Histone H3 (di methyl K27) peptide (ab1781) at 1 μg/ml

Secondary
Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 17 kDa

Exposure time : 1 minute

All lanes:

Western blot - Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-di-methyl-k9-antibody-mabcam-1220-chip-grade-ab1220'>ab1220</a>) at 1 µg/mL

Lanes 1, 3, 4, 5, 6 and 7:

Calf thymus histone lysate

Lane 2:

Calf thymus histone lysate with Histone H3 peptide - unmodified

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/rabbit-mouse-igg-h-l-hrp-ab6728'>ab6728</a>) at 1/5000 dilution

Predicted band size: 15 kDa

true

Exposure time: 1min

Key facts

Purity

>90% HPLC

Applications

applications

Biologically active

Accession

P68431

Animal free

Carrier free

Species

Human

Storage buffer

pH: 7.4 Constituents: 0.87% Sodium chloride, 0.476% HEPES, 0.0584% EDTA, 0.001% Sorbitan monolaurate, ethoxylated

storage-buffer

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "BL": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn't dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

style

left-column

Sequence info

[{"sequence":null,"proteinLength":null,"predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Synthetic","expressionSystem":null,"accessionNumber":"P68431","tags":[]}]

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

False

style

left-column

style

left-column

Specifications

Form

Liquid

style

left-column

General info

Function

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

Sequence similarities

Belongs to the histone H3 family.

Post-translational modifications

Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.. Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.. Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.. Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin. Monomethylation at Lys-57 (H3K56me1) by EHMT2/G9A in G1 phase promotes interaction with PCNA and is required for DNA replication.. Phosphorylated at Thr-4 (H3T3ph) by VRK1 (PubMed:31527692). Phosphorylated at Thr-4 (H3T3ph) by HASPIN during prophase and dephosphorylated during anaphase (PubMed:15681610, PubMed:16185088). Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MAP3K20 isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 or isoform M2 of PKM (PKM2) is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Thr-12 (H3T11ph) by chromatin-associated CHEK1 regulates the transcription of cell cycle regulatory genes by modulating acetylation of Lys-10 (H3K9ac). Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.. Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.. Lysine deamination at Lys-5 (H3K4all) to form allysine is mediated by LOXL2. Allysine formation by LOXL2 only takes place on H3K4me3 and results in gene repression.. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.. Butyrylation of histones marks active promoters and competes with histone acetylation. It is present during late spermatogenesis.. Succinylation at Lys-80 (H3K79succ) by KAT2A takes place with a maximum frequency around the transcription start sites of genes (PubMed:29211711). It gives a specific tag for epigenetic transcription activation (PubMed:29211711). Desuccinylation at Lys-123 (H3K122succ) by SIRT7 in response to DNA damage promotes chromatin condensation and double-strand breaks (DSBs) repair (PubMed:27436229).. Serine ADP-ribosylation by PARP1 or PARP2 constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed:30257210, PubMed:34874266). Serine ADP-ribosylation at Ser-11 (H3S10ADPr) promotes recruitment of CHD1L (PubMed:34874266). H3S10ADPr is mutually exclusive with phosphorylation at Ser-11 (H3S10ph) and impairs acetylation at Lys-10 (H3K9ac) (PubMed:30257210).. Serotonylated by TGM2 at Gln-6 (H3Q5ser) during serotonergic neuron differentiation (PubMed:30867594). H3Q5ser is associated with trimethylation of Lys-5 (H3K4me3) and enhances general transcription factor IID (TFIID) complex-binding to H3K4me3, thereby facilitating transcription (PubMed:30867594).. Dopaminylated by TGM2 at Gln-6 (H3Q5dop) in ventral tegmental area (VTA) neurons (PubMed:32273471). H3Q5dop mediates neurotransmission-independent role of nuclear dopamine by regulating relapse-related transcriptional plasticity in the reward system (By similarity).. Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription.

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Publications (9)

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eLife 8: PubMed31573510

2019

H3K9me2 orchestrates inheritance of spatial positioning of peripheral heterochromatin through mitosis.

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Andrey Poleshko,Cheryl L Smith,Son C Nguyen,Priya Sivaramakrishnan,Karen G Wong,John Isaac Murray,Melike Lakadamyali,Eric F Joyce,Rajan Jain,Jonathan A Epstein

Cell 171:573-587.e14 PubMed29033129

2017

Genome-Nuclear Lamina Interactions Regulate Cardiac Stem Cell Lineage Restriction.

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Andrey Poleshko,Parisha P Shah,Mudit Gupta,Apoorva Babu,Michael P Morley,Lauren J Manderfield,Jamie L Ifkovits,Damelys Calderon,Haig Aghajanian,Javier E Sierra-Pagán,Zheng Sun,Qiaohong Wang,Li Li,Nicole C Dubois,Edward E Morrisey,Mitchell A Lazar,Cheryl L Smith,Jonathan A Epstein,Rajan Jain

Oncotarget 7:39796-39808 PubMed27174920

2016

Inhibition of EHMT2/G9a epigenetically increases the transcription of Beclin-1 via an increase in ROS and activation of NF-κB.

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Sang Eun Park,Hye Jin Yi,Nayoung Suh,Yun-Yong Park,Jae-Young Koh,Seong-Yun Jeong,Dong-Hyung Cho,Choung-Soo Kim,Jung Jin Hwang

PLoS genetics 7:e1002434 PubMed22242006

2011

The RNA silencing enzyme RNA polymerase v is required for plant immunity.

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Ana López,Vicente Ramírez,Javier García-Andrade,Victor Flors,Pablo Vera

Journal of immunological methods 344:58-63 PubMed19328803

2009

A streamlined method for rapid and sensitive chromatin immunoprecipitation.

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Michael L Sikes,Justin M Bradshaw,Wendell T Ivory,Jessica L Lunsford,Ruth E McMillan,Clayton R Morrison

The Journal of biological chemistry 283:36542-52 PubMed18984585

2008

The histone demethylases JMJD1A and JMJD2B are transcriptional targets of hypoxia-inducible factor HIF.

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Sophie Beyer,Malene Maag Kristensen,Kim Steen Jensen,Jens Vilstrup Johansen,Peter Staller

Molecular cell 20:971-8 PubMed16364921

2005

Eaf3 chromodomain interaction with methylated H3-K36 links histone deacetylation to Pol II elongation.

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Amita A Joshi,Kevin Struhl

Molecular and cellular biology 25:1511-25 PubMed15684400

2005

Chromatin architecture near a potential 3' end of the igh locus involves modular regulation of histone modifications during B-Cell development and in vivo occupancy at CTCF sites.

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Francine E Garrett,Alexander V Emelyanov,Manuel A Sepulveda,Patrick Flanagan,Sabrina Volpi,Fubin Li,Dmitry Loukinov,Laurel A Eckhardt,Victor V Lobanenkov,Barbara K Birshtein

Proceedings of the National Academy of Sciences of 101:17450-5 PubMed15574503

2004

Multiple spatially distinct types of facultative heterochromatin on the human inactive X chromosome.

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Brian P Chadwick,Huntington F Willard

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Human Histone H3 (di methyl K9) peptide

ChIP - Human Histone H3 (di methyl K9) peptide (AB1772)

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

Western blot - Human Histone H3 (di methyl K9) peptide (AB1772)

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