JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB1782

Human Histone H3 (tri methyl K27) peptide

5

(1 Review)

|

(11 Publications)

Human Histone H3 (tri methyl K27) peptide is a Synthetic blocking peptide. >70% and suitable for Dot, BL.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K27me3, H3K27me, H3K27

14 Images
Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)
  • WB

Project

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)

Expected molecular weights : H3 = 17 kDa; H4 = 14 kDa This image shows that the main epitopes recognized by ab7315 are the di methylated lysine residues. This can be seen in lanes 4, 7 and 10 where the activity of the antibody is quenched by the immunizing peptides (ab7768, ab1772, ab1781).

All lanes:

Western blot - Anti-pan methyl Lysine antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/pan-methyl-lysine-antibody-chip-grade-ab7315'>ab7315</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k4-peptide-ab1342'>ab1342</a>)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k9-peptide-ab1773'>ab1773</a>)

Lane 9:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 10:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Lane 11:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K27) peptide (ab1782)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 14-17 kDa

true

Exposure time: 20min

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)
  • WB

Unknown

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)

All lanes:

Western blot - Anti-Histone H3 (di methyl K4, tri methyl K4) antibody [mAbcam 6000] - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-di-methyl-k4-tri-methyl-k4-antibody-mabcam-6000-chip-grade-ab6000'>ab6000</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k4-peptide-ab1342'>ab1342</a>)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k9-peptide-ab1773'>ab1773</a>)

Lane 9:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 10:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Lane 11:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K27) peptide (ab1782)

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/10000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

true

Exposure time: 20min

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)
  • WB

Project

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)

All lanes:

Western blot - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-di-methyl-k36-antibody-chip-grade-ab9049'>ab9049</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Histone H3 peptide (ab41430)

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K36) peptide (<a href='/en-us/products/unavailable/human-histone-h3-mono-methyl-k36-peptide-ab1783'>ab1783</a>)

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K36) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k36-peptide-ab1784'>ab1784</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K36) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k36-peptide-ab1785'>ab1785</a>)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k9-peptide-ab1773'>ab1773</a>)

Lane 9:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 10:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Lane 11:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K27) peptide (ab1782)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)
  • WB

Unknown

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)

The peptide competition assay shows that this antibody is very specific for Histone H3 di-methylated at K27. It does not recognise Histone H3 mono, di, or tri methylated at K4, Histone H3 mono, di, or tri methylated at K9, or Histone H3 mono, or tri methylated at K27.

All lanes:

Western blot - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-di-methyl-k27-antibody-chip-grade-ab24684'>ab24684</a>) at 1 µg/mL

Lane 1:

Calf thymus histone lysate

Lane 2:

Calf thymus histone lysate with Human Histone H3 peptide (<a href='/en-us/products/unavailable/human-histone-h3-peptide-ab17163'>ab17163</a>)

Lane 3:

Calf thymus histone lysate with Human Histone H3 (mono methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

Lane 4:

Calf thymus histone lysate with Human Histone H3 (di methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf thymus histone lysate with Human Histone H3 (tri methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k4-peptide-ab1342'>ab1342</a>)

Lane 6:

Calf thymus histone lysate with Human Histone H3 (mono methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 7:

Calf thymus histone lysate with Human Histone H3 (di methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Calf thymus histone lysate with Human Histone H3 (tri methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k9-peptide-ab1773'>ab1773</a>)

Lane 9:

Calf thymus histone lysate with Human Histone H3 (mono methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 10:

Calf thymus histone lysate with Human Histone H3 (di methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Lane 11:

Calf thymus histone lysate with Human Histone H3 (tri methyl K27) peptide (ab1782)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG H&L (HRP) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Exposure time: 5s

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)
  • WB

Unknown

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)

Rabbit polyclonal to Histone H3 K9 Methyl K9 (1/1000)

Peptides at 1 ug/ml

1XTBS, 5%BSA, 0.5% Tween

This antibody shows significantly greater reactivity with mono methyl K9. This can be seen in lane 7, as the addition of ab1771 (mono methyl K9) completely blocks the activity of ab9045. Weaker cross-reactivity is seen against mono methyl K27. This is shown in lane 3, as the addition of ab1780 only partially blocks the activity of ab9045.

All lanes:

Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-mono-methyl-k9-antibody-chip-grade-ab9045'>ab9045</a>) at 1 µg/mL

Predicted band size: 15 kDa

false

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)
  • WB

Project

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)

All lanes:

Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-mono-methyl-k9-antibody-chip-grade-ab9045'>ab9045</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate (<a href='/en-us/products/unavailable/calf-thymus-histone-preparation-nuclear-lysate-ab121'>ab121</a>) at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate (<a href='/en-us/products/unavailable/calf-thymus-histone-preparation-nuclear-lysate-ab121'>ab121</a>) at 0.5 µg with Human Histone H3 (unmodified) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate (<a href='/en-us/products/unavailable/calf-thymus-histone-preparation-nuclear-lysate-ab121'>ab121</a>) at 0.5 µg with Human Histone H3 (mono methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate (<a href='/en-us/products/unavailable/calf-thymus-histone-preparation-nuclear-lysate-ab121'>ab121</a>) at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate (<a href='/en-us/products/unavailable/calf-thymus-histone-preparation-nuclear-lysate-ab121'>ab121</a>) at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k4-peptide-ab1342'>ab1342</a>)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate (<a href='/en-us/products/unavailable/calf-thymus-histone-preparation-nuclear-lysate-ab121'>ab121</a>) at 0.5 µg with Human Histone H3 (mono methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate (<a href='/en-us/products/unavailable/calf-thymus-histone-preparation-nuclear-lysate-ab121'>ab121</a>) at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate (<a href='/en-us/products/unavailable/calf-thymus-histone-preparation-nuclear-lysate-ab121'>ab121</a>) at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k9-peptide-ab1773'>ab1773</a>)

Lane 9:

Calf Thymus Histone Preparation Nuclear Lysate (<a href='/en-us/products/unavailable/calf-thymus-histone-preparation-nuclear-lysate-ab121'>ab121</a>) at 0.5 µg with Human Histone H3 (mono methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 10:

Calf Thymus Histone Preparation Nuclear Lysate (<a href='/en-us/products/unavailable/calf-thymus-histone-preparation-nuclear-lysate-ab121'>ab121</a>) at 0.5 µg with Human Histone H3 (di methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Lane 11:

Calf Thymus Histone Preparation Nuclear Lysate (<a href='/en-us/products/unavailable/calf-thymus-histone-preparation-nuclear-lysate-ab121'>ab121</a>) at 0.5 µg with Human Histone H3 (tri methyl K27) peptide (ab1782)

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 15 kDa

false

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)
  • WB

Project

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)

ab8896 specifically recognises mono-methyl K9 (lane 1) in calf thymus lysate at 17kDa. ab8896 is successfully blocked using the immunizing peptide (lane 2 ab1771), but not the di-methyl K9 (lane 3 ab1772), the tri-methyl K9 (lane 4 ab1773), the mono-methyl K27 (lane 5 ab1780), the di-methyl K27 (lane 6 ab1781), the tri-methyl K27 (lane 7 ab1782), the mono-methyl K4 (lane 8 ab1340) nor the corresponding unmodified peptides (lane 9 ab7228, lane 10 ab2623). This implies that ab8896 is specific to mono-methyl K9.

All lanes:

Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-mono-methyl-k9-antibody-chip-grade-ab8896'>ab8896</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation at 0.5 µg with Human Histone H3 (mono methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 3:

Calf Thymus Histone Preparation at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 4:

Calf Thymus Histone Preparation at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k9-peptide-ab1773'>ab1773</a>)

Lane 5:

Calf Thymus Histone Preparation at 0.5 µg with Human Histone H3 (mono methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 6:

Calf Thymus Histone Preparation at 0.5 µg with Human Histone H3 (di methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Lane 7:

Calf Thymus Histone Preparation at 0.5 µg with Human Histone H3 (tri methyl K27) peptide (ab1782)

Lane 8:

Calf Thymus Histone Preparation at 0.5 µg with Human Histone H3 (mono methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

Lane 9:

Calf Thymus Histone Preparation at 0.5 µg with Human Histone H3 (unmodified) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 10:

Calf Thymus Histone Preparation at 0.5 µg with Human Histone H3 (unmodified ) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab2623'>ab2623</a>)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)
  • WB

Lab

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)

Lane 8 shows that Rabbit polyclonal to Histone H3 (tri methyl K9) is blocked by the addition of the immunizing peptide (ab1773). Cross-reactivity with Histone H3 peptide - tri methyl K27 (ab1782) is also shown in Lane 11.

All lanes:

Western blot - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-tri-methyl-k9-antibody-chip-grade-ab8898'>ab8898</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k4-peptide-ab1342'>ab1342</a>)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k9-peptide-ab1773'>ab1773</a>)

Lane 9:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 10:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Lane 11:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K27) peptide (ab1782)

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)
  • WB

Unknown

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)

ab31399 specifically recognises mono-methyl K27 (lane 1). ab31399 is successfully blocked using the immunizing peptide (lane 3 ab1780), but not the corresponding unmodified peptides (lane 2 ab2903, lane 6 ab2623) nor di-methyl K27 (lane 4 ab1781), tri-methyl K27 (lane 5 ab1782), mono-methyl K9 (lane 7 ab1771), mono-methyl K4 (lane 8 ab1340) peptides.

All lanes:

Anti-Histone H3 (mono methyl K27) antibody (<a href='/en-us/products/unavailable/histone-h3-mono-methyl-k27-antibody-ab31399'>ab31399</a>) at 1 µg/mL

Lane 1:

HeLa nuclear lysate at 0.5 µg

Lane 2:

HeLa nuclear lysate at 0.5 µg with Human Histone H3 (unmodified ) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab2903'>ab2903</a>)

Lane 3:

HeLa nuclear lysate at 0.5 µg with Human Histone H3 (mono methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 4:

HeLa nuclear lysate at 0.5 µg with Human Histone H3 (di methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Lane 5:

HeLa nuclear lysate at 0.5 µg with Human Histone H3 (tri methyl K27) peptide (ab1782)

Lane 6:

HeLa nuclear lysate at 0.5 µg with Human Histone H3 (unmodified ) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab2623'>ab2623</a>)

Lane 7:

HeLa nuclear lysate at 0.5 µg with Human Histone H3 (mono methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 8:

HeLa nuclear lysate at 0.5 µg with Human Histone H3 (mono methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

false

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)
  • WB

Ap

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)

All lanes:

Western blot - HRP Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] (<a href='/en-us/products/primary-antibodies/hrp-histone-h3-di-methyl-k9-antibody-mabcam-1220-ab62168'>ab62168</a>) at 1/20000 dilution

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified ) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab2903'>ab2903</a>)

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k4-peptide-ab1342'>ab1342</a>)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k9-peptide-ab1773'>ab1773</a>)

Lane 9:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 10:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Lane 11:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K27) peptide (ab1782)

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)
  • WB

Lab

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab6147 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (<a href='/en-us/products/primary-antibodies/histone-h3-di-methyl-k27-tri-methyl-k27-antibody-mabcam-6147-ab6147'>ab6147</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified ) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab2623'>ab2623</a>)

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k4-peptide-ab1342'>ab1342</a>)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k9-peptide-ab1773'>ab1773</a>)

Lane 9:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 10:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Lane 11:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K27) peptide (ab1782)

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

true

Exposure time: 1min

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)
  • WB

Unknown

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)

ab31388 specifically recognises mono-methyl K27 (lane 1). ab31388 is successfully blocked using the immunizing peptide (lane 3 ab1780), but not the corresponding unmodified peptides (lane 2 ab2903, lane 6 ab2623) nor di-methyl K27 (lane 4 ab1781), tri-methyl K27 (lane 5 ab1782), mono-methyl K9 (lane 7 ab1771), mono-methyl K4 (lane 8 ab1340) peptides.
The bands at 50 kDa are attributed to non-specific binding of the antibody.

All lanes:

Anti-Histone H3 (mono methyl K27) antibody (<a href='/en-us/products/unavailable/histone-h3-mono-methyl-k27-antibody-ab31388'>ab31388</a>) at 1 µg/mL

Lane 1:

HeLa nuclear lysate at 2.5 µg

Lane 2:

HeLa nuclear lysate at 2.5 µg with Human Histone H3 (unmodified ) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab2903'>ab2903</a>)

Lane 3:

HeLa nuclear lysate at 2.5 µg with Human Histone H3 (mono methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 4:

HeLa nuclear lysate at 2.5 µg with Human Histone H3 (di methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Lane 5:

HeLa nuclear lysate at 2.5 µg with Human Histone H3 (tri methyl K27) peptide (ab1782)

Lane 6:

HeLa nuclear lysate at 2.5 µg with Human Histone H3 (unmodified ) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab2623'>ab2623</a>)

Lane 7:

HeLa nuclear lysate at 2.5 µg with Human Histone H3 (mono methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 8:

HeLa nuclear lysate at 2.5 µg with Human Histone H3 (mono methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

false

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)
  • WB

Project

Western blot - Human Histone H3 (tri methyl K27) peptide (AB1782)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine, and sodium azide (ab12209).

All lanes:

Western blot - Anti-Histone H3 (tri methyl K4) antibody [mAbcam12209] - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-tri-methyl-k4-antibody-mabcam12209-chip-grade-ab12209'>ab12209</a>) at 2 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg

Lane 2:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (unmodified) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 3:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k4-peptide-ab1340'>ab1340</a>)

Lane 4:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 5:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k4-peptide-ab1342'>ab1342</a>)

Lane 6:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 7:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 8:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k9-peptide-ab1773'>ab1773</a>)

Lane 9:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k27-peptide-ab1780'>ab1780</a>)

Lane 10:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (di methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Lane 11:

Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K27) peptide (ab1782)

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

false

Exposure time: 16min

Dot Blot - Human Histone H3 (tri methyl K27) peptide (AB1782)
  • Dot

AbReview21279****

Dot Blot - Human Histone H3 (tri methyl K27) peptide (AB1782)

ab1782 (at 0.01 and 0.1 μg) used in dot blot to test for cross-reactivity of an anti-H3K27me3 polyclonal antibody (1/2500). A HRP-conjugated mouse anti-rabbit polyclonal (1/10000) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Key facts

Purity

>70% HPLC

Tags

Tag free

Applications

BL, Dot

applications

Biologically active

No

Accession

P68431

Animal free

No

Carrier free

No

Species

Human

style
left-column

Complementary Products

Sample Prep & Detection Kits

AB113476

Histone Extraction Kit

Functional Studies - Histone Extraction Kit (AB113476)

  • 4
  • 6
Primary Antibodies

AB8898

Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (AB8898)

  • 5
  • 92
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "BL": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn't dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
style
left-column

Sequence info

[{"sequence":null,"proteinLength":null,"predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Synthetic","expressionSystem":null,"accessionNumber":"P68431","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
-20°C
False
style
left-column
style
left-column

Specifications

Form

Lyophilized

Additional notes

70 - 90% by HPLC

style
left-column

General info

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Sequence similarities

Belongs to the histone H3 family.

Post-translational modifications

Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.. Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.. Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.. Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin. Monomethylation at Lys-57 (H3K56me1) by EHMT2/G9A in G1 phase promotes interaction with PCNA and is required for DNA replication.. Phosphorylated at Thr-4 (H3T3ph) by VRK1 (PubMed:31527692). Phosphorylated at Thr-4 (H3T3ph) by HASPIN during prophase and dephosphorylated during anaphase (PubMed:15681610, PubMed:16185088). Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MAP3K20 isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 or isoform M2 of PKM (PKM2) is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Thr-12 (H3T11ph) by chromatin-associated CHEK1 regulates the transcription of cell cycle regulatory genes by modulating acetylation of Lys-10 (H3K9ac). Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.. Monoubiquitinated at Lys-15 (H3K14ub), Lys-19 (H3K18ub) and Lys-24 (H3K23ub) by UHRF1 and HLTF at hemimethylated DNA sites following DNA replication, promoting recruitment of DNMT1 DNA methyltransferase and replication-coupled DNA methylation maintenance (PubMed:27595565, PubMed:29053958). Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation (PubMed:16678110). This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins (PubMed:16678110).. Lysine deamination at Lys-5 (H3K4all) to form allysine is mediated by LOXL2. Allysine formation by LOXL2 only takes place on H3K4me3 and results in gene repression.. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.. Butyrylation of histones marks active promoters and competes with histone acetylation. It is present during late spermatogenesis.. Succinylation at Lys-80 (H3K79succ) by KAT2A takes place with a maximum frequency around the transcription start sites of genes (PubMed:29211711). It gives a specific tag for epigenetic transcription activation (PubMed:29211711). Desuccinylation at Lys-123 (H3K122succ) by SIRT7 in response to DNA damage promotes chromatin condensation and double-strand breaks (DSBs) repair (PubMed:27436229).. Serine ADP-ribosylation by PARP1 or PARP2 constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed:30257210, PubMed:34874266). Serine ADP-ribosylation at Ser-11 (H3S10ADPr) promotes recruitment of CHD1L (PubMed:34874266). H3S10ADPr is mutually exclusive with phosphorylation at Ser-11 (H3S10ph) and impairs acetylation at Lys-10 (H3K9ac) (PubMed:30257210).. Serotonylated by TGM2 at Gln-6 (H3Q5ser) during serotonergic neuron differentiation (PubMed:30867594). H3Q5ser is associated with trimethylation of Lys-5 (H3K4me3) and enhances general transcription factor IID (TFIID) complex-binding to H3K4me3, thereby facilitating transcription (PubMed:30867594).. Dopaminylated by TGM2 at Gln-6 (H3Q5dop) in ventral tegmental area (VTA) neurons (PubMed:32273471). H3Q5dop mediates neurotransmission-independent role of nuclear dopamine by regulating relapse-related transcriptional plasticity in the reward system (By similarity).. Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H3C1 tri methyl K27
style
left-column

Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Epigenetics & chromatin 17:3 PubMed38336688

2024

A low-input high resolution sequential chromatin immunoprecipitation method captures genome-wide dynamics of bivalent chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Janith A Seneviratne,William W H Ho,Eleanor Glancy,Melanie A Eckersley-Maslin

eLife 9: PubMed32804637

2020

nuclear RNAi factor SET-32 deposits the transgenerational histone modification, H3K23me3.

Applications

Unspecified application

Species

Unspecified reactive species

Lianna Schwartz-Orbach,Chenzhen Zhang,Simone Sidoli,Richa Amin,Diljeet Kaur,Anna Zhebrun,Julie Ni,Sam G Gu

Nature communications 7:12514 PubMed27530917

2016

reChIP-seq reveals widespread bivalency of H3K4me3 and H3K27me3 in CD4(+) memory T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Kinkley,Johannes Helmuth,Julia K Polansky,Ilona Dunkel,Gilles Gasparoni,Sebastian Fröhler,Wei Chen,Jörn Walter,Alf Hamann,Ho-Ryun Chung

PloS one 10:e0143595 PubMed26630262

2015

Characterization of Proliferating Neural Progenitors after Spinal Cord Injury in Adult Zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Subhra Prakash Hui,Tapas Chandra Nag,Sukla Ghosh

BMC medical genomics 8:74 PubMed26553048

2015

Histone methylation levels correlate with TGFBIp and extracellular matrix gene expression in normal and granular corneal dystrophy type 2 corneal fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Sun Maeng,Ga-Hyun Lee,Seung-Il Choi,Kyu Seo Kim,Eung Kweon Kim

Cell death & disease 5:e1554 PubMed25476902

2014

Decreased H3K27 and H3K4 trimethylation on mortal chromosomes in distributed stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Y H Huh,J L Sherley

PloS one 9:e112899 PubMed25397898

2014

Pdsg1 and Pdsg2, novel proteins involved in developmental genome remodelling in Paramecium.

Applications

Unspecified application

Species

Unspecified reactive species

Miroslav Arambasic,Pamela Y Sandoval,Cristina Hoehener,Aditi Singh,Estienne C Swart,Mariusz Nowacki

Epigenetics 9:222-35 PubMed24169366

2013

In situ histone landscape of nephrogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan McLaughlin,Fenglin Wang,Zubaida Saifudeen,Samir S El-Dahr

Developmental biology 355:227-38 PubMed21549110

2011

Lineage specific trimethylation of H3 on lysine 4 during C. elegans early embryogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Siyao Wang,Kate Fisher,Gino B Poulin

The Journal of biological chemistry 281:8888-97 PubMed16373353

2005

Dynamic changes in histone H3 lysine 9 methylations: identification of a mitosis-specific function for dynamic methylation in chromosome congression and segregation.

Applications

Unspecified application

Species

Unspecified reactive species

Kirk J McManus,Vincent L Biron,Ryan Heit,D Alan Underhill,Michael J Hendzel
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com