Human Histone H3 (tri methyl K27) peptide is a Synthetic blocking peptide. >=70% purity and suitable for Dot, BL.
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- eLife 9:2020nuclear RNAi factor SET-32 deposits the transgenerational histone modification, H3K23me3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLianna Schwartz-Orbach et. al.PubMed 32804637
- Nature communications 7:125142016reChIP-seq reveals widespread bivalency of H3K4me3 and H3K27me3 in CD4(+) memory T cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Kinkley et. al.PubMed 27530917
- PloS one 10:e01435952015Characterization of Proliferating Neural Progenitors after Spinal Cord Injury in Adult Zebrafish.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSubhra Prakash Hui,Tapas Chandra Nag,Sukla GhoshPubMed 26630262
- BMC medical genomics 8:742015Histone methylation levels correlate with TGFBIp and extracellular matrix gene expression in normal and granular corneal dystrophy type 2 corneal fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong-Sun Maeng et. al.PubMed 26553048
- Cell death & disease 5:e15542014Decreased H3K27 and H3K4 trimethylation on mortal chromosomes in distributed stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesY H Huh et. al.PubMed 25476902
- PloS one 9:e1128992014Pdsg1 and Pdsg2, novel proteins involved in developmental genome remodelling in Paramecium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiroslav Arambasic et. al.PubMed 25397898
- Epigenetics 9:222-352013In situ histone landscape of nephrogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNathan McLaughlin,Fenglin Wang,Zubaida Saifudeen,Samir S El-DahrPubMed 24169366
- Developmental biology 355:227-382011Lineage specific trimethylation of H3 on lysine 4 during C. elegans early embryogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiyao Wang et. al.PubMed 21549110
- The Journal of biological chemistry 281:8888-972005Dynamic changes in histone H3 lysine 9 methylations: identification of a mitosis-specific function for dynamic methylation in chromosome congression and segregation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKirk J McManus et. al.PubMed 16373353
- Proceedings of the National Academy of Sciences of 101:17450-52004Multiple spatially distinct types of facultative heterochromatin on the human inactive X chromosome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrian P Chadwick et. al.PubMed 15574503
Key facts
- Purity
- >=70% HPLC
- Tags
- Tag free
- Applications
- Dot, BL
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Application BL | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K27me3
Recommended products
Human Histone H3 (tri methyl K27) peptide is a Synthetic blocking peptide. >=70% purity and suitable for Dot, BL.
Key facts
- Purity
- >=70% HPLC
- Tags
- Tag free
- Applications
- Dot, BL
- Biologically active
- No
- Accession
- P68431-1
- Animal free
- No
- Species
- Human
- Concentration
Specifications
- Form
- Lyophilized
- Additional notes
70 - 90% by HPLC
General info
- Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
- Sequence similarities
Belongs to the histone H3 family.
- Post-translational modifications
Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- -20°C
Notes
- If the peptide doesn't dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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15 product images
This image is courtesy of an anonymous customer reviewDot Blot - Human Histone H3 (tri methyl K27) peptide (ab1782)
ab1782 (at 0.01 and 0.1 μg) used in dot blot to test for cross-reactivity of an anti-H3K27me3 polyclonal antibody (1/2500). A HRP-conjugated mouse anti-rabbit polyclonal (1/10000) was used as the secondary antibody.
Western blot - Human Histone H3 (tri methyl K27) peptide (ab1782)
ab31388 specifically recognises mono-methyl K27 (lane 1). ab31388 is successfully blocked using the immunizing peptide (lane 3 Human Histone H3 (mono methyl K27) peptide ab1780), but not the corresponding unmodified peptides (lane 2 Human Histone H3 (unmodified ) peptide ab2903, lane 6 Human Histone H3 (unmodified ) peptide ab2623) nor di-methyl K27 (lane 4 Human Histone H3 (di methyl K27) peptide ab1781), tri-methyl K27 (lane 5 ab1782), mono-methyl K9 (lane 7 Human Histone H3 (mono methyl K9) peptide ab1771), mono-methyl K4 (lane 8 Human Histone H3 (mono methyl K4) peptide ab1340) peptides.
The bands at 50 kDa are attributed to non-specific binding of the antibody.All lanes: Anti-Histone H3 (mono methyl K27) antibody (ab31388) at 1 µg/mL
All lanes: HeLa nuclear lysate at 2.5 µg
Western blot - Human Histone H3 (tri methyl K27) peptide (ab1782)
The peptide competition assay shows that this antibody is very specific for Histone H3 di-methylated at K27. It does not recognise Histone H3 mono
, di
, or tri methylated at K4
, Histone H3 mono
, di
, or tri methylated at K9
, or Histone H3 mono
, or tri methylated at K27.
All lanes: Western blot - Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (Anti-Histone H3 (di methyl K27) antibody - ChIP Grade ab24684) at 1 µg/mL
Lanes 1, 3, 4, 5, 6, 7, 8, 9, 10 and 11: Calf thymus histone lysate
Lane 2: Calf thymus histone lysate with Human Histone H3 peptide (ab17163)
SecondaryAll lanes: Goat polyclonal to Rabbit IgG H&L (HRP) at 1/5000 dilution
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 5s
Western blot - Human Histone H3 (tri methyl K27) peptide (ab1782)
Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade ab8896 specifically recognises mono-methyl K9 (lane 1) in calf thymus lysate at 17kDa. Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade ab8896 is successfully blocked using the immunizing peptide (lane 2 Human Histone H3 (mono methyl K9) peptide ab1771), but not the di-methyl K9 (lane 3 Human Histone H3 (di methyl K9) peptide ab1772), the tri-methyl K9 (lane 4 Human Histone H3 (tri methyl K9) peptide ab1773), the mono-methyl K27 (lane 5 Human Histone H3 (mono methyl K27) peptide ab1780), the di-methyl K27 (lane 6 Human Histone H3 (di methyl K27) peptide ab1781), the tri-methyl K27 (lane 7 ab1782), the mono-methyl K4 (lane 8 Human Histone H3 (mono methyl K4) peptide ab1340) nor the corresponding unmodified peptides (lane 9 Human Histone H3 (unmodified) peptide ab7228, lane 10 Human Histone H3 (unmodified ) peptide ab2623). This implies that Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade ab8896 is specific to mono-methyl K9.
All lanes: Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade ab8896) at 1 µg/mL
All lanes: Calf Thymus Histone Preparation at 0.5 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Western blot - Human Histone H3 (tri methyl K27) peptide (ab1782)
Lane 8 shows that Rabbit polyclonal to Histone H3 (tri methyl K9) is blocked by the addition of the immunizing peptide (Human Histone H3 (tri methyl K9) peptide ab1773). Cross-reactivity with Histone H3 peptide - tri methyl K27 (ab1782) is also shown in Lane 11.
All lanes: Western blot - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade ab8898) at 1 µg/mL
All lanes: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Western blot - Human Histone H3 (tri methyl K27) peptide (ab1782)
All lanes: Western blot - Anti-Histone H3 (di methyl K36) antibody - ChIP Grade (Anti-Histone H3 (di methyl K36) antibody - ChIP Grade ab9049) at 1 µg/mL
Lanes 1, 4, 5, 6, 7, 8, 9, 10 and 11: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
Lane 2: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Histone H3 peptide (ab41430)
Lane 3: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (mono methyl K36) peptide (ab1783)
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Western blot - Human Histone H3 (tri methyl K27) peptide (ab1782)
Rabbit polyclonal to Histone H3 K9 Methyl K9 (1/1000)
Peptides at 1 ug/ml
1XTBS, 5%BSA, 0.5% Tween
This antibody shows significantly greater reactivity with mono methyl K9. This can be seen in lane 7, as the addition of Human Histone H3 (mono methyl K9) peptide ab1771 (mono methyl K9) completely blocks the activity of Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade ab9045. Weaker cross-reactivity is seen against mono methyl K27. This is shown in lane 3, as the addition of Human Histone H3 (mono methyl K27) peptide ab1780 only partially blocks the activity of Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade ab9045.
All lanes: Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade ab9045) at 1 µg/mL
Predicted band size: 15 kDa
Western blot - Human Histone H3 (tri methyl K27) peptide (ab1782)
All lanes: Western blot - Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade (Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade ab9045) at 1 µg/mL
All lanes: Calf Thymus Histone Preparation Nuclear Lysate (ab121) at 0.5 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Western blot - Human Histone H3 (tri methyl K27) peptide (ab1782)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine, and sodium azide (Anti-Histone H3 (tri methyl K4) antibody [mAbcam12209] - ChIP Grade ab12209).
All lanes: Western blot - Anti-Histone H3 (tri methyl K4) antibody [mAbcam12209] - ChIP Grade (Anti-Histone H3 (tri methyl K4) antibody [mAbcam12209] - ChIP Grade ab12209) at 2 µg/mL
All lanes: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
SecondaryAll lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 16min
Western blot - Human Histone H3 (tri methyl K27) peptide (ab1782)
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] ab6147 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] (Anti-Histone H3 (di methyl K27, tri methyl K27) antibody [mAbcam 6147] ab6147) at 1 µg/mL
All lanes: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
SecondaryAll lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 1min
Western blot - Human Histone H3 (tri methyl K27) peptide (ab1782)
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade ab6002 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade ab6002) at 1 µg/mL
Lanes 1, 3, 4, 5, 6, 7, 8, 9, 10 and 11: Calf Thymus Histone Preparation Nuclear Lysate at 0.25 µg
Lane 2: Calf Thymus Histone Preparation Nuclear Lysate at 0.25 µg with Human Histone H3 peptide (ab17163)
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 3min
Western blot - Human Histone H3 (tri methyl K27) peptide (ab1782)
All lanes: Western blot - Anti-Histone H3 (di methyl K4, tri methyl K4) antibody [mAbcam 6000] - ChIP Grade (Anti-Histone H3 (di methyl K4, tri methyl K4) antibody [mAbcam 6000] - ChIP Grade ab6000) at 1 µg/mL
All lanes: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 20min
Western blot - Human Histone H3 (tri methyl K27) peptide (ab1782)
ab31399 specifically recognises mono-methyl K27 (lane 1). ab31399 is successfully blocked using the immunizing peptide (lane 3 Human Histone H3 (mono methyl K27) peptide ab1780), but not the corresponding unmodified peptides (lane 2 Human Histone H3 (unmodified ) peptide ab2903, lane 6 Human Histone H3 (unmodified ) peptide ab2623) nor di-methyl K27 (lane 4 Human Histone H3 (di methyl K27) peptide ab1781), tri-methyl K27 (lane 5 ab1782), mono-methyl K9 (lane 7 Human Histone H3 (mono methyl K9) peptide ab1771), mono-methyl K4 (lane 8 Human Histone H3 (mono methyl K4) peptide ab1340) peptides.
All lanes: Anti-Histone H3 (mono methyl K27) antibody (ab31399) at 1 µg/mL
All lanes: HeLa nuclear lysate at 0.5 µg
Western blot - Human Histone H3 (tri methyl K27) peptide (ab1782)
Expected molecular weights: H3 = 17 kDa; H4 = 14 kDa
This image shows that the main epitopes recognized by Anti-pan methyl Lysine antibody - ChIP Grade ab7315 are the di methylated lysine residues. This can be seen in lanes 4, 7 and 10 where the activity of the antibody is quenched by the immunizing peptides (Human Histone H3 (di methyl K4) peptide ab7768, Human Histone H3 (di methyl K9) peptide ab1772, Human Histone H3 (di methyl K27) peptide ab1781).All lanes: Western blot - Anti-pan methyl Lysine antibody - ChIP Grade (Anti-pan methyl Lysine antibody - ChIP Grade ab7315) at 1 µg/mL
All lanes: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14-17 kDa
Exposure time: 20min
Western blot - Human Histone H3 (tri methyl K27) peptide (ab1782)
All lanes: Western blot - HRP Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] (HRP Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] ab62168) at 1/20000 dilution
All lanes: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa
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