Human Histone H4 peptide is a Synthetic blocking peptide. >90% purity and suitable for BL.
Images
Key facts
- Purity
- >90% HPLC
- Tags
- Tag free
- Applications
- BL
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application BL | Reactivity Reacts | Dilution info 1 µg/mL | Notes - |
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Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4, H4K8cr
Recommended products
Human Histone H4 peptide is a Synthetic blocking peptide. >90% purity and suitable for BL.
Key facts
- Purity
- >90% HPLC
- Tags
- Tag free
- Applications
- BL
- Biologically active
- No
- Accession
- P62805-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
Constituents: HEPES, 0.87% Sodium chloride, 0.0584% EDTA, 0.001% Sorbitan monolaurate, ethoxylated
Specifications
- Form
- Liquid
General info
- Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
- Sequence similarities
Belongs to the histone H4 family.
- Post-translational modifications
Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
- If the peptide doesn't dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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2 product images
Western blot - Human Histone H4 peptide (ab13843)
All lanes: Western blot - Anti-Histone H4 antibody - ChIP Grade (Anti-Histone H4 antibody - ChIP Grade ab10158) at 1 µg/mL
Lanes 1 and 3: Histone prep at 20 µg
Lane 2: HeLa histone lysate at 20 µg
Lane 4: HeLa Histone lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/5000 dilution
Performed under reducing conditions.
Western blot - Human Histone H4 peptide (ab13843)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (Anti-Histone H4 antibody [mAbcam 31830] - ChIP Grade ab31830).
All lanes: Western blot - Anti-Histone H4 antibody [mAbcam 31830] - ChIP Grade (Anti-Histone H4 antibody [mAbcam 31830] - ChIP Grade ab31830) at 5 µg/mL
Lanes 1 and 8: Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
Lanes 2 and 9: HeLa Histone Preparation Nuclear Lysate at 2.5 µg
Lanes 3 and 10: Histone H4 Recombinant Protein at 0.1 µg
Lanes 4 and 11: Histone H3.1 Recombinant Protein at 0.1 µg
Lanes 5 and 12: Histone H2A Recombinant Protein at 0.1 µg
Lanes 6 and 13: Histone H2B Recombinant Protein at 0.1 µg
Lanes 7 and 14: Histone H1 Recombinant Protein at 0.1 µg
SecondaryAll lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Exposure time: 2min
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