Human IgE protein (Azide free)
4
(2 Reviews)
|
(12 Publications)
Human IgE protein (Azide free) is a Human Full Length IgE protein with >90% purity, < 25 EU/mg endotoxin level and suitable for western blot, ELISA, IHC, ICC and SDS-PAGE. The predicted molecular weight of ab65866 protein is 185 kDa.
- Save time and ensure accurate results - use ab65866 as an IgE isotype control
- Optimal bioactivity, stability and reproducibility
- No contamination from other immunoglobulin isotypes
- Available in different sizes to fit your experimental needs
View Alternative Names
Immunoglobulin epsilon heavy chain, Immunoglobulin epsilon heavy chain ND
- SDS-PAGE
Supplier Data
SDS-PAGE - Human IgE protein (Azide free) (AB65866)
SDS-PAGE analysis of :
Lane 1 : Molecular weight markers.
Lane 2 : ab65866 at molecular weight 185 kDa.
Lane 3 : IgE purified from serum from a myeloma patient.
- sELISA
Unknown
Sandwich ELISA - Human IgE protein (Azide free) (AB65866)
Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Ensure the validity of your result using our Immunoglobulin E (IgE) protein ab65866 as a control.
IgE protein ab65866 can be used as a positive control in SDS-PAGE and western blot assays.
Analyze your ELISA data using the IgE ab65866 protein to generate and plot the standard curve.
Check out our ELISA protocol for more information here.
This product is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.
This antigen is quite unique, because it is the only available IgE product, which originates from a human monoclonal cell line. Our IgE product shows superior advantage when compared to the traditionally purified polyclonal IgE from myeloma patients. A comparison which is highly superior on the following key parameters:
- Low batch-to-batch variation
- Higher degree of purity (>90%)
- A more uniform product (monoclonal and not polyclonal)
Low endotoxin levels below < 25 EU/mg allow use of IgE protein in cellular immunology and in cell proliferation studies.
Ensure the validity of your result using our Immunoglobulin E (IgE) protein ab65866 as an isotype control. ab65866 can be used as a positive control in SDS-PAGE and western blot assays.
Analyze your ELISA data using the IgE ab65866 protein to generate and plot the standard curve.
Check out our ELISA protocol for more information here https://www.abcam.com/en-us/technical-resources/protocols/indirect-and-direct-elisa.
This product is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.
This antigen is quite unique, because it is the only available IgE product, which originates from a human monoclonal cell line.
Our IgE product shows superior advantage when compared to the traditionally purified polyclonal IgE from myeloma patients.
A comparison which is highly superior on the following key parameters:
- Low batch-to-batch variation
- Higher degree of purity (>90%)
- A more uniform product (monoclonal and not polyclonal)
Low endotoxin levels below < 25 EU/mg allow use of IgE protein in cellular immunology and in cell proliferation studies.
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The IgE protein functions as a mediator in allergic reactions. It does not operate as part of a complex but directly interacts with allergens and the IgE-specific receptors on immune cells. IgE's role is important in defense against parasitic infections where it performs a protective function by promoting eosinophil activation and degranulation. This action helps the body to expel the parasites. In the absence of parasitic infection IgE's function translates into its role in allergic responses where it becomes responsible for the symptoms associated with allergic diseases.
Pathways
IgE is an important player in the immune response pathway. The protein interacts with allergy-related pathways such as the T helper 2 (Th2) pathway. IgE's role in this pathway involves cooperation with cytokines notably IL-4 and IL-13 which are produced by Th2 cells and promote IgE synthesis in B cells. Also IgE is linked to the FcεRI receptor signaling pathway that initiates the release of histamines and other mediators when IgE binds to an allergen leading to an allergic response.
Specifications
Form
Liquid
Additional notes
Human IgE full length protein was purified by Protein L chromatography. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from Foetal Bovine Serum.
General info
Function
Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (12)
Recent publications for all applications. Explore the full list and refine your search
BMC biotechnology 24:72 PubMed39367362
2024
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The Journal of allergy and clinical immunology 152:711-724.e14 PubMed37100120
2023
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International journal of molecular medicine 46:1367-1376 PubMed32945368
2020
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Journal of immunotoxicology 17:110-121 PubMed32525431
2020
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Nucleic acids research 48:e63 PubMed32383757
2020
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Langmuir : the ACS journal of surfaces and colloids 34:10321-10332 PubMed30089209
2018
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PloS one 13:e0191987 PubMed29389948
2018
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Journal of immunological methods 455:41-54 PubMed29397157
2018
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Biochemical and biophysical research communications 483:669-673 PubMed27988336
2016
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JAMA dermatology 153:30-38 PubMed27829102
2016
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Product promise
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