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Human IgE protein (Azide free) is a Human Full Length protein, expressed in Native, with >90% purity, < 25 EU/mg endotoxin level and suitable for IHC - Wmt, ELISA, WB, ICC, SDS-PAGE.

Purity

>90% SDS-PAGE

Endotoxin level

< 25 EU/mg

Expression system

Native

Tags

Tag free

Applications

IHC - Wmt, ELISA, WB, ICC, SDS-PAGE

Biologically active

Yes

Reactivity data

Application
IHC - Wmt
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ICC
Reactivity
Reacts
Dilution info
-
Notes

-

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

Observed band of ~185kDa on SDS-PAGE

Images

Target data

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Human IgE protein (Azide free) is a Human Full Length protein, expressed in Native, with >90% purity, < 25 EU/mg endotoxin level and suitable for IHC - Wmt, ELISA, WB, ICC, SDS-PAGE.

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 25 EU/mg

Expression system

Native

Applications

IHC - Wmt, ELISA, WB, ICC, SDS-PAGE

Biological activity

1 mg of ab65866 is 380,000± 50,000 IU/ml.

Accession
P24001-4
Protein length

Full Length

Animal free

No

Carrier free

Yes

Species

Human

Concentration
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Storage buffer

pH: 7.4
Constituents: 0.88% Sodium chloride, 0.00017% PBS

Specifications

Form

Liquid

Additional notes

Human IgE full length protein was purified by Protein L chromatography. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from Foetal Bovine Serum.

General info

Function

Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

This product is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.

This antigen is quite unique, because it is the only available IgE product, which originates from a human monoclonal cell line. Our IgE product shows superior advantage when compared to the traditionally purified polyclonal IgE from myeloma patients. A comparison which is highly superior on the following key parameters:.

- Low batch-to-batch variation

- Higher degree of purity (>90%)

- A more uniform product (monoclonal and not polyclonal)

Low endotoxin levels below < 25 EU/mg allow use of IgE protein in cellular immunology and in cell proliferation studies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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