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Human non-muscle Myosin IIA peptide is a Synthetic blocking peptide. >70% purity and suitable for BL.
Alternative names=Myosin-9, Myosin heavy chain 9, Non-muscle myosin heavy chain A, Non-muscle myosin heavy chain IIa, NMMHC-A, NMMHC II-a, NMMHC-IIA, MYH9
- Purity
>70% HPLC
- Tags
Tag free
- Applications
BL
- Biologically active
No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application BL | Reactivity Reacts | Dilution info - | Notes - |
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Human non-muscle Myosin IIA peptide is a Synthetic blocking peptide. >70% purity and suitable for BL.
Alternative names=Myosin-9, Myosin heavy chain 9, Non-muscle myosin heavy chain A, Non-muscle myosin heavy chain IIa, NMMHC-A, NMMHC II-a, NMMHC-IIA, MYH9
Key facts
- Purity
>70% HPLC
- Tags
Tag free
- Applications
BL
- Biologically active
No
- Accession
- P35579-1
- Animal free
No
- Nature
Synthetic
- Species
Human
- Concentration
- Storage buffer
Constituents: 0.87% Sodium chloride, 0.714% HEPES, 0.0584% EDTA, 0.001% Tween
Specifications
- Form
Lyophilized
- Additional notes
70 - 90% by HPLC
General info
- Function
Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Required for cortical actin clearance prior to oocyte exocytosis (By similarity). Promotes cell motility in conjunction with S100A4 (PubMed:16707441). During cell spreading, plays an important role in cytoskeleton reorganization, focal contact formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10 (PubMed:20052411).
- Sequence similarities
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.
- Post-translational modifications
ISGylated.
- Subcellular localisation
Cytoskeleton
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn't dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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