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AB15581

Ki67 peptide

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(6 Publications)

Ki67 peptide is a Synthetic blocking peptide. >90% and suitable for BL.

View Alternative Names

Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67

1 Images
Western blot - Ki67 peptide (AB15581)
  • WB

Project

Western blot - Ki67 peptide (AB15581)

Observed band sizes : 345kDa, 395kDa

All lanes:

Western blot - Anti-Ki67 antibody (<a href='/en-us/products/primary-antibodies/ki67-antibody-ab15580'>ab15580</a>) at 1 µg/mL

All lanes:

Hela whole cell lysate at 20 µg

Secondary

All lanes:

Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution

false

Key facts

Purity

>90% HPLC

Tags

Tag free

Applications

BL

applications

Biologically active

No

Accession

P46013

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 6.75 Constituents: 0.87% Sodium chloride, 0.714% HEPES, 0.0584% EDTA, 0.001% Sorbitan monolaurate, ethoxylated

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "BL": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1 µg/mL", "notes":"<p></p>" } } }
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Product details

- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn't dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Sequence info

[{"sequence":null,"proteinLength":null,"predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Synthetic","expressionSystem":null,"accessionNumber":"P46013","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

The protein expressed by the MKI67 gene is required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly and associates with the surface of the mitotic chromosome, specifically the perichromosomal layer, covering a significant portion of the chromosome surface (PubMed : 27362226). It prevents chromosomes from collapsing into a single chromatin mass by creating a steric and electrostatic charge barrier due to its high net electrical charge, acting as a surfactant that disperses chromosomes and enables their independent motility (PubMed : 27362226). The protein binds DNA, with a preference for supercoiled and AT-rich DNA (PubMed : 10878551), and may play a role in chromatin organization, though it is unclear if it directly influences chromatin organization or if this is an indirect result of its function in maintaining dispersed mitotic chromosomes (Probable, PubMed : 24867636). This supplementary information is collated from multiple sources and compiled automatically.

Post-translational modifications

Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.

Subcellular localisation

Nucleus

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Product protocols

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Target data

The protein expressed by the MKI67 gene is required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly and associates with the surface of the mitotic chromosome, specifically the perichromosomal layer, covering a significant portion of the chromosome surface (PubMed : 27362226). It prevents chromosomes from collapsing into a single chromatin mass by creating a steric and electrostatic charge barrier due to its high net electrical charge, acting as a surfactant that disperses chromosomes and enables their independent motility (PubMed : 27362226). The protein binds DNA, with a preference for supercoiled and AT-rich DNA (PubMed : 10878551), and may play a role in chromatin organization, though it is unclear if it directly influences chromatin organization or if this is an indirect result of its function in maintaining dispersed mitotic chromosomes (Probable, PubMed : 24867636). This supplementary information is collated from multiple sources and compiled automatically.
See full target information MKI67
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Materials today. Bio 32:101851 PubMed40487169

2025

Integrating gene demethylation and immune modulation: PD-1 nanovesicles as a dual-action therapy for NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Zhang,Yuan Liang,Shiyang Deng,Jiang Du

Neoplasma 70:272-286 PubMed37226932

2023

NUCKS1 promotes the progression of colorectal cancer via activating PI3K/AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liao-Liao Zhu,Jing-Jie Shi,Yong-Dong Guo,Cheng Yang,Rong-Lin Wang,Shan-Shan Li,Dong-Xue Gan,Pei-Xiang Ma,Jun-Qiang Li,Hai-Chuan Su

Science advances 6:eaay6324 PubMed31998844

2020

Microglia response following acute demyelination is heterogeneous and limits infiltrating macrophage dispersion.

Applications

Unspecified application

Species

Unspecified reactive species

Jason R Plemel,Jo Anne Stratton,Nathan J Michaels,Khalil S Rawji,Eric Zhang,Sarthak Sinha,Charbel S Baaklini,Yifei Dong,Madelene Ho,Kevin Thorburn,Timothy N Friedman,Sana Jawad,Claudia Silva,Andrew V Caprariello,Vahid Hoghooghi,Julie Yue,Arzina Jaffer,Kelly Lee,Bradley J Kerr,Raj Midha,Peter K Stys,Jeff Biernaskie,V Wee Yong

The Journal of comparative neurology 527:1654-1674 PubMed30552685

2018

Cortical interlaminar astrocytes across the therian mammal radiation.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen Falcone,Marisol Wolf-Ochoa,Sarwat Amina,Tiffany Hong,Gelareh Vakilzadeh,William D Hopkins,Patrick R Hof,Chet C Sherwood,Paul R Manger,Stephen C Noctor,Verónica Martínez-Cerdeño

Journal of vascular surgery 67:933-944.e3 PubMed29477204

2018

MicroRNA-155 promotes neointimal hyperplasia through smooth muscle-like cell-derived RANTES in arteriovenous fistulas.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Wang,Peng Deng,Yuan Sun,Ping Ye,Anchen Zhang,Chuangyan Wu,Zhang Yue,Zhaolei Chen,Jiahong Xia

Clinical cancer research : an official journal of the American Association for Cancer Research 16:212-25 PubMed20008843

2009

Quantified tumor t1 is a generic early-response imaging biomarker for chemotherapy reflecting cell viability.

Applications

BL

Species

Unspecified reactive species

Paul M J McSheehy,Claudia Weidensteiner,Catherine Cannet,Stephane Ferretti,Didier Laurent,Stephan Ruetz,Michael Stumm,Peter R Allegrini
View all publications
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