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AB326317

Marburg-Musoke virus-like particles (MMARV VLP)

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Marburg-Musoke virus-like particles (MMARV VLP) is a Marburg virus - Musoke, Kenya, 1980 protein, expressed in Sf9 cells, with >75%, suitable for WB.

View Alternative Names

Matrix protein VP40, Marburg VP40, Membrane-associated protein VP40, mVP40

1 Images
Western blot - Marburg-Musoke virus-like particles (MMARV VLP) (AB326317)
  • WB

Supplier Data

Western blot - Marburg-Musoke virus-like particles (MMARV VLP) (AB326317)

Western Blot was used to confirm the presence of MMARV-specific GP, NP and VP40 in the VLP. VLP sample was heat-denatured under reducing condition and loaded at 1 µg.

(A) GP2 containing protein bands are visualized using 0.5 µg/mL of anti-MARV GP rabbit pAb. GP is highly glycosylated and most often detected at a molecular weight higher than the theoretical size due to the altered mobility.

(B) NP was detected as a prominent band at ~90 kDa and other NP-containing fragments, using 0.5 µg/mL of anti-MARV NP rabbit pAb.

(C) VP40 was detected as a prominent band at ~35 kDa and other VP40-containing fragments, using 0.5 µg/mL of anti-MARV VP40 rabbit pAb.

All lanes:

Western blot - Marburg-Musoke virus-like particles (MMARV VLP) (ab326317) at 1 µg

Observed band size: 35 kDa,90 kDa

false

Key facts

Purity

>75% SDS-PAGE

Expression system

Sf9 cells

Tags

Tag free

Applications

WB

applications

Biologically active

No

Accession

P35260

Animal free

No

Carrier free

No

Species

Marburg virus - Musoke, Kenya, 1980

Storage buffer

Constituents: PBS, 10% Glycerol (glycerin, glycerine), 0.9% L-Arginine, 0.7% Glutamic Acid

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1 µg", "notes":"<p></p>" } } }

Product details

Virus-like Particles (VLP’s) expressing recombinant Marburg (MARV) glycoprotein (GP), nucleoprotein (NP), and matrix protein (VP40). Since these VLP’s mimic MARV (Musoke) virus but do not contain genetic material, thus are not infectious making an ideal candidate as a vaccine and a tool to enhance filovirus research.

Sequence info

[{"linker":null,"sequence":"","proteinLength":null,"predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Sf9 cells","accessionNumber":"P35260","tags":[]}]

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage duration
A few weeks
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False

Specifications

Form

Liquid

General info

Function

Plays an essential role virus particle assembly and budding (PubMed : 33673144). Promotes virus assembly and budding by interacting with host proteins of the multivesicular body pathway. The interaction with host E3 ubiquitin ligase SMURF2 facilitates virus budding (PubMed : 33673144). The interaction with the nucleocapsid and the plasma membrane may also facilitate virus budding. Specific interactions with membrane-associated GP and VP24 during the budding process may also occur (By similarity). May play a role in genome replication (By similarity).

Sequence similarities

Belongs to the filoviridae matrix protein VP40 family.

Subcellular localisation

Host late endosome membrane

Product protocols

Target data

Plays an essential role virus particle assembly and budding (PubMed : 33673144). Promotes virus assembly and budding by interacting with host proteins of the multivesicular body pathway. The interaction with host E3 ubiquitin ligase SMURF2 facilitates virus budding (PubMed : 33673144). The interaction with the nucleocapsid and the plasma membrane may also facilitate virus budding. Specific interactions with membrane-associated GP and VP24 during the budding process may also occur (By similarity). May play a role in genome replication (By similarity).
See full target information VP40

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